Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
352,300 |
C→T |
56.0% |
T124I (ACT→ATT) |
prpE → |
predicted propionyl‑CoA synthetase with ATPase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 352,300 | 0 | C | T | 56.0%
| 12.7
/ 65.4
| 50 | T124I (ACT→ATT) | prpE | predicted propionyl‑CoA synthetase with ATPase domain |
| Reads supporting (aligned to +/- strand): ref base C (18/4); new base T (17/11); total (35/15) |
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 |
TCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCG > W3110S.gb/352233‑352368
|
tCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTActct > 1:1497344/1‑71 (MQ=255)
tCATTGGGTGTGCAGCGCGGCGATCGGGTGATGGTGTATATGCCGATGATTGCCGAAGCGCATATTActct > 1:2533500/1‑71 (MQ=255)
gcgcGGCGATCGGGTGCTGGTGTATATGTCGATGATT‑CCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg < 1:1338403/71‑1 (MQ=255)
cgcgGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg < 1:700955/71‑1 (MQ=255)
gcggcgATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg > 1:53844/1‑70 (MQ=255)
tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCTCGCGCATTGgcg < 1:2857612/71‑1 (MQ=255)
tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGgcg < 1:450090/71‑1 (MQ=255)
tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGgcg < 1:1929319/71‑1 (MQ=255)
tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCATGCGCGCGCATTGgcg < 1:3078492/71‑1 (MQ=255)
cGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGgcgc > 1:2603523/1‑71 (MQ=255)
tGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt < 1:3126381/71‑1 (MQ=255)
tGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt < 1:787111/71‑1 (MQ=255)
cTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTgg > 1:2741759/1‑46 (MQ=255)
ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTctg > 1:77728/1‑40 (MQ=255)
ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTgcgcgcgc > 1:1152591/1‑55 (MQ=255)
ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACt > 1:2783054/1‑71 (MQ=255)
tataTGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCa < 1:2009968/52‑1 (MQ=255)
tatGCCGATGATTGCCTAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:165788/1‑71 (MQ=255)
tatGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:3078191/1‑71 (MQ=255)
tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTgg > 1:789884/1‑38 (MQ=255)
tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAt > 1:3533329/1‑51 (MQ=255)
tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:3025676/1‑71 (MQ=255)
tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGTGCGCGCATTGGCGCTATTCACTCGgtggtg < 1:1775729/71‑1 (MQ=255)
tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAttt > 1:3427659/1‑50 (MQ=255)
tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:1499472/1‑71 (MQ=255)
tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:2436255/1‑71 (MQ=255)
tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:1595744/1‑71 (MQ=255)
gCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:417989/1‑71 (MQ=255)
gCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:857098/1‑71 (MQ=255)
ccGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGtt > 1:903459/1‑71 (MQ=255)
ccGATGATTGCCGAAGCGCATATTACTCTGCCGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGtt > 1:309222/1‑71 (MQ=255)
gatTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTa > 1:1833650/1‑50 (MQ=255)
gatTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTa > 1:34763/1‑50 (MQ=255)
ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1661925/71‑1 (MQ=255)
ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1321888/71‑1 (MQ=255)
ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1925626/71‑1 (MQ=255)
tGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:2240960/1‑51 (MQ=255)
gCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:259484/1‑50 (MQ=255)
gCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:3658911/1‑50 (MQ=255)
gCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTtgg > 1:735636/1‑65 (MQ=255)
gCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTtgg > 1:1131432/1‑65 (MQ=255)
gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:1982132/1‑70 (MQ=255)
gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:373659/1‑70 (MQ=255)
gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:3751076/1‑70 (MQ=255)
gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:2234443/1‑70 (MQ=255)
gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:1778622/1‑70 (MQ=255)
cATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg < 1:1113305/49‑1 (MQ=255)
attattCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTAGGTGGTGTTTGGTGGATTTGCCTCGCACAg > 1:3294268/1‑71 (MQ=255)
ttATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:3655808/1‑48 (MQ=255)
ttATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:2476058/1‑48 (MQ=255)
ttACTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt > 1:2323556/1‑35 (MQ=255)
ttACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTCGCCTCGCACAg < 1:1463528/70‑1 (MQ=255)
tACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCg > 1:134388/1‑71 (MQ=255)
|
TCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCG > W3110S.gb/352233‑352368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
