Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,600,711 |
A→G |
20.5% |
S405P (TCA→CCA) |
ydeV ← |
predicted sugar kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,600,711 | 0 | A | G | 20.5%
| 78.0
/ 17.3
| 39 | S405P (TCA→CCA) | ydeV | predicted sugar kinase |
| Reads supporting (aligned to +/- strand): ref base A (14/17); new base G (4/4); total (18/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
CCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGA > W3110S.gb/1600641‑1600780
|
ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:1075266/1‑71 (MQ=255)
ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:964143/1‑71 (MQ=255)
ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:3001596/1‑71 (MQ=255)
cTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACa < 1:1218422/71‑1 (MQ=255)
aaaGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcg < 1:3284446/71‑1 (MQ=255)
aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt > 1:1169776/1‑71 (MQ=255)
aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt > 1:3952452/1‑71 (MQ=255)
aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATA‑AATCGCCGCAtttt > 1:265528/1‑71 (MQ=255)
aTGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttct < 1:2437056/71‑1 (MQ=255)
tGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGGTACa < 1:2301241/55‑1 (MQ=255)
gAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttcttc > 1:3147052/1‑71 (MQ=255)
tGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt < 1:4084180/60‑1 (MQ=255)
tatTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACg > 1:190292/1‑70 (MQ=255)
ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:1399200/50‑1 (MQ=255)
ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:2722049/50‑1 (MQ=255)
ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:4026531/50‑1 (MQ=255)
cGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAAc < 1:3081216/70‑1 (MQ=255)
aGCAATTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCtt > 1:199982/1‑71 (MQ=255)
aGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACaa > 1:3237690/1‑63 (MQ=255)
aGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCCTCCAGCGCACGGAACaa > 1:1470589/1‑63 (MQ=255)
aaTTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGt < 1:2432964/71‑1 (MQ=255)
tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGt > 1:1657332/1‑69 (MQ=255)
tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:3952494/71‑1 (MQ=255)
tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:1637608/71‑1 (MQ=255)
tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:2513975/71‑1 (MQ=255)
tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:2717988/71‑1 (MQ=255)
tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:3084192/1‑71 (MQ=255)
tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:1637417/1‑71 (MQ=255)
tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:1522866/1‑71 (MQ=255)
ctgcAAGTTACACGCTTGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa < 1:1925082/69‑1 (MQ=255)
cAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACAttt < 1:1410306/69‑1 (MQ=255)
cAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTa < 1:3298074/70‑1 (MQ=255)
aGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATcc < 1:356018/71‑1 (MQ=255)
gTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAAc < 1:626026/46‑1 (MQ=255)
tACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCggg > 1:50604/1‑71 (MQ=255)
acacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGt > 1:3113335/1‑71 (MQ=255)
cacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTc < 1:2554408/71‑1 (MQ=255)
cacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTc < 1:500572/71‑1 (MQ=255)
gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:2208368/1‑46 (MQ=255)
gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:419731/1‑46 (MQ=255)
gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:658108/1‑46 (MQ=255)
gATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGa > 1:2226293/1‑71 (MQ=255)
aTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATgg < 1:4045216/69‑1 (MQ=255)
|
CCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGA > W3110S.gb/1600641‑1600780
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
