Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,701,562:1 |
+C |
28.9% |
coding (494/1593 nt) |
malX → |
fused maltose and glucose‑specific PTS enzyme IIBC components |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,701,562 | 1 | . | C | 28.9%
| 50.2
/ 49.8
| 38 | coding (494/1593 nt) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base . (5/22); new base C (4/7); total (9/29) |
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 |
GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATT > W3110S.gb/1701494‑1701623
|
gTCGATCGATACCGGGTTCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cc < 1:375731/71‑1 (MQ=255)
gTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cc < 1:114868/71‑1 (MQ=255)
tcgatcgaTACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCa < 1:691533/71‑1 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:3189147/71‑4 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1866546/71‑4 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1741184/71‑4 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1774363/71‑4 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:307637/71‑4 (MQ=255)
gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCata < 1:3392466/71‑1 (MQ=255)
agatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataata > 1:1533367/2‑71 (MQ=255)
gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:2124215/71‑1 (MQ=255)
gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:2181304/71‑1 (MQ=255)
gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:207021/71‑1 (MQ=255)
tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATcc < 1:2307717/64‑1 (MQ=255)
tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:2780041/71‑1 (MQ=255)
tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:3168083/71‑1 (MQ=255)
tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:1161616/71‑1 (MQ=255)
ggtgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTgg < 1:1237063/71‑1 (MQ=255)
tgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCat < 1:608009/44‑1 (MQ=255)
tgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCTCTGGCa < 1:521789/71‑1 (MQ=255)
gATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAatat > 1:3968160/1‑49 (MQ=39)
gATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAatat > 1:3330645/1‑49 (MQ=39)
aTCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:2266413/57‑1 (MQ=255)
aTCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:416592/57‑1 (MQ=255)
attatCGTCTGGATGCTGCATGAGCTTTTCCCataat < 1:2292596/37‑1 (MQ=25)
attatCGTCTGGATGCTGCATGAGCGTTTCCCataat < 1:1475198/37‑1 (MQ=37)
ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:609882/60‑1 (MQ=255)
ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:2363559/70‑1 (MQ=255)
atCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcgg > 1:2461851/1‑66 (MQ=255)
atCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcgg > 1:1172078/1‑66 (MQ=255)
atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGa > 1:1790589/1‑47 (MQ=255)
atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAc > 1:3798595/1‑71 (MQ=255)
tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAcg > 1:3298985/1‑71 (MQ=255)
tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAcg > 1:2553804/1‑71 (MQ=255)
tgcATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:141194/45‑1 (MQ=255)
tgcATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:2717378/45‑1 (MQ=255)
cATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCa < 1:2027429/69‑1 (MQ=255)
tGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:1274208/71‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAttc < 1:209012/43‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAttc < 1:1847423/43‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCtt < 1:2720150/60‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCtt < 1:2613372/60‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:717167/70‑1 (MQ=255)
tGAGCGTTT‑CCATAATATCCGCCTGCCAGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:1356962/70‑1 (MQ=255)
gAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:2316627/69‑1 (MQ=255)
ttt‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:1829524/45‑1 (MQ=255)
|
GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATT > W3110S.gb/1701494‑1701623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
