Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,291,258 |
C→T |
22.1% |
G326D (GGC→GAC) |
yejO ← |
predicted autotransporter outer membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,291,258 | 0 | C | T | 22.1%
| 179.4
/ 45.8
| 86 | G326D (GGC→GAC) | yejO | predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base T (14/5); total (44/42) |
| Fisher's exact test for biased strand distribution p-value = 3.70e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 |
ATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291191‑2291327
|
atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAg > 1:1510954/1‑71 (MQ=255)
atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg < 1:3836804/71‑1 (MQ=255)
atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg < 1:688525/71‑1 (MQ=255)
atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:1406722/1‑71 (MQ=255)
atttatttGCGGCTTCATGACCTCAAGAATGACATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:390152/1‑71 (MQ=255)
ggCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:873679/71‑1 (MQ=255)
ttCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:1961444/68‑1 (MQ=255)
ttCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:3647569/68‑1 (MQ=255)
tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgc > 1:1166176/1‑70 (MQ=255)
aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCgg > 1:425819/1‑69 (MQ=255)
aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCgg > 1:240296/1‑69 (MQ=255)
aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGc < 1:639565/70‑1 (MQ=255)
ccTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGcc < 1:2692357/70‑1 (MQ=255)
cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGcc > 1:1368258/1‑46 (MQ=255)
cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttg > 1:597159/1‑71 (MQ=255)
cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:3845873/1‑71 (MQ=255)
cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:2723746/1‑71 (MQ=255)
cAAGAATGCCATCATTCCCTCTGACAGAAACGTTAACCATTGTGcc > 1:2163773/1‑46 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3577867/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3556835/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:4054018/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttg < 1:866564/70‑1 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:1914550/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:2063970/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3119246/1‑71 (MQ=255)
aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:906101/69‑1 (MQ=255)
aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:3249729/71‑1 (MQ=255)
aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:978114/71‑1 (MQ=255)
aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:4002852/71‑1 (MQ=255)
gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:3211482/71‑1 (MQ=255)
gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:3405355/71‑1 (MQ=255)
gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:466798/71‑1 (MQ=255)
gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:2175019/71‑1 (MQ=255)
aTGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGa > 1:469088/1‑70 (MQ=255)
tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:3506122/65‑1 (MQ=255)
tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:3930963/65‑1 (MQ=255)
tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAc < 1:2578963/68‑1 (MQ=255)
tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCat < 1:2841277/71‑1 (MQ=255)
catTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAt > 1:2387504/1‑57 (MQ=255)
catTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAt > 1:3735726/1‑57 (MQ=255)
catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:2851752/64‑1 (MQ=255)
catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCata > 1:305417/1‑71 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3344535/1‑59 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:1798640/1‑71 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:62889/1‑71 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCata > 1:1233237/1‑70 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa > 1:4006200/1‑68 (MQ=255)
atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa > 1:3161638/1‑68 (MQ=255)
atTCCCTCTGACTGAAACGTTAACAATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:107768/1‑59 (MQ=255)
cccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:1623475/1‑71 (MQ=255)
cccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:1795460/1‑71 (MQ=255)
cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:494950/1‑71 (MQ=255)
cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:2807425/1‑71 (MQ=255)
cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCAATTGTTGATAACCATAtttt > 1:706179/1‑71 (MQ=255)
ccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:2508411/1‑61 (MQ=255)
ccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:3306353/1‑61 (MQ=255)
ctctGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:1560102/40‑1 (MQ=255)
ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:1871888/1‑69 (MQ=255)
ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2161234/1‑69 (MQ=255)
ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2283477/1‑69 (MQ=255)
ctgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:1375700/1‑69 (MQ=255)
ctgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:4026478/1‑69 (MQ=255)
tgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:772565/1‑68 (MQ=255)
actgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:3031240/68‑1 (MQ=255)
actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:3027385/68‑1 (MQ=255)
actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1781568/68‑1 (MQ=255)
actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1309238/68‑1 (MQ=255)
actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGAt < 1:836628/71‑1 (MQ=255)
ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg < 1:669344/71‑1 (MQ=255)
ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg < 1:190365/71‑1 (MQ=255)
tgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1704810/66‑1 (MQ=255)
aCGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCttt > 1:1442150/1‑71 (MQ=255)
cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAgaga < 1:2485672/63‑1 (MQ=255)
cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAgaga < 1:3036438/63‑1 (MQ=255)
cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGc > 1:799919/1‑67 (MQ=255)
cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt < 1:224552/71‑1 (MQ=255)
gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:2239529/71‑1 (MQ=255)
gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:93666/71‑1 (MQ=255)
gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:93096/71‑1 (MQ=255)
gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:847583/71‑1 (MQ=255)
ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:783461/38‑1 (MQ=255)
ttAACCATTGTGCCAGACCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt > 1:1383695/1‑69 (MQ=255)
aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg < 1:3031230/70‑1 (MQ=255)
ccATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAc < 1:152542/43‑1 (MQ=255)
gtgtCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:178682/1‑37 (MQ=255)
gtgtCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt > 1:3011863/1‑71 (MQ=255)
gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt > 1:2729456/1‑71 (MQ=255)
gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGACGAATACGt > 1:3922380/1‑71 (MQ=255)
tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt < 1:3316919/70‑1 (MQ=255)
tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTT‑AGGAATACGTGa < 1:4084165/71‑1 (MQ=255)
ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGAt < 1:3857783/50‑1 (MQ=255)
ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGa < 1:1874439/70‑1 (MQ=255)
ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGa < 1:2797666/70‑1 (MQ=255)
|
ATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291191‑2291327
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
