Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,993,568 |
C→A |
25.4% |
intergenic (‑9/+25) |
ygeK ← / ← ygeL |
predicted DNA‑binding transcriptional regulator/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,993,568 | 0 | C | A | 25.4%
| 113.6
/ 19.1
| 47 | intergenic (‑9/+25) | ygeK/ygeL | predicted DNA‑binding transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/20); new base A (7/5); total (22/25) |
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 |
ATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCA > W3110S.gb/2993504‑2993633
|
ataataTGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCaaa < 1:2290656/71‑1 (MQ=255)
atatGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATtc < 1:509075/71‑1 (MQ=255)
atatGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAAtt > 1:2838512/1‑70 (MQ=255)
tGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTcc > 1:375068/1‑71 (MQ=255)
tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:916358/1‑70 (MQ=255)
tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:3885763/1‑70 (MQ=255)
tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:3350505/1‑70 (MQ=255)
tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:2370894/1‑70 (MQ=255)
tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:2258999/1‑70 (MQ=255)
atctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa > 1:1212702/1‑71 (MQ=255)
atctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAAtt > 1:970417/1‑58 (MQ=255)
tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1077825/70‑1 (MQ=255)
tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataa < 1:3110198/69‑1 (MQ=255)
tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataa < 1:1412918/69‑1 (MQ=255)
tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAaaataaa < 1:391321/70‑1 (MQ=255)
ctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:820296/67‑1 (MQ=255)
cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAAtt > 1:1338947/1‑50 (MQ=255)
cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAgg > 1:3414988/1‑71 (MQ=255)
cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAaataaata > 1:2063690/1‑65 (MQ=255)
cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAaataaata > 1:3703676/1‑65 (MQ=255)
tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:1165534/71‑1 (MQ=255)
tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:2778673/71‑1 (MQ=255)
tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:3681277/71‑1 (MQ=255)
aCCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTa < 1:1556324/69‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:2126233/51‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:2168986/62‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTTTAGATTTGt < 1:847010/71‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTTTAGATTTGt < 1:632370/71‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:2253906/71‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:3667456/71‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:3151944/71‑1 (MQ=255)
ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:637533/71‑1 (MQ=255)
ttACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:4018395/1‑71 (MQ=255)
ttACGAGTTCGGCCCCCATCATTCCACACGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:2623176/1‑71 (MQ=255)
gTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1101330/43‑1 (MQ=255)
gTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1840041/43‑1 (MQ=255)
ccATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCt < 1:2274598/71‑1 (MQ=255)
atcatTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGa > 1:1401652/1‑47 (MQ=255)
tcatTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa < 1:3783547/53‑1 (MQ=255)
catTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttct > 1:4057697/1‑71 (MQ=255)
atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:1254659/1‑71 (MQ=255)
atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:1249926/1‑71 (MQ=255)
atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:3501801/1‑71 (MQ=255)
atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:919866/1‑71 (MQ=255)
ttCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTg < 1:4027305/47‑1 (MQ=255)
ccACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:351514/1‑48 (MQ=255)
ccACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCa < 1:1371553/70‑1 (MQ=255)
|
ATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCA > W3110S.gb/2993504‑2993633
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
