Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,053,087 |
T→A |
29.7% |
Y137F (TAT→TTT) |
pepP ← |
proline aminopeptidase P II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,053,087 | 0 | T | A | 29.7%
| 70.4
/ 35.9
| 47 | Y137F (TAT→TTT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base T (14/19); new base A (8/6); total (22/25) |
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTGAT > W3110S.gb/3053020‑3053157
|
tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:1885360/71‑1 (MQ=255)
cggtgAGATTTTGCCGCGTACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca < 1:1948994/71‑1 (MQ=255)
cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:3094693/1‑71 (MQ=255)
cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:1548510/1‑71 (MQ=255)
cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:1622733/1‑71 (MQ=255)
cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:3752664/1‑71 (MQ=255)
tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata > 1:1355928/1‑58 (MQ=255)
tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGccc > 1:475115/1‑71 (MQ=255)
tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGccc > 1:1926650/1‑71 (MQ=255)
ttGACGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTAGCCCt > 1:2512147/1‑71 (MQ=255)
cgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:3565007/53‑1 (MQ=255)
ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGt > 1:3418881/1‑70 (MQ=255)
ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTa < 1:3147513/71‑1 (MQ=255)
ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTa < 1:3234897/71‑1 (MQ=255)
tttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg < 1:2259841/62‑1 (MQ=255)
tACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTaaa > 1:2698254/1‑69 (MQ=255)
aGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:455364/71‑1 (MQ=255)
aGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTg > 1:2047341/1‑53 (MQ=255)
tttttCCAGCGCACTGTTCACGATTACATCAgcat < 1:320915/35‑1 (MQ=255)
tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:1629379/70‑1 (MQ=255)
tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:354897/70‑1 (MQ=255)
tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:3841753/70‑1 (MQ=255)
tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:502776/70‑1 (MQ=255)
tttatCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:2558724/70‑1 (MQ=255)
tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc > 1:1394703/1‑67 (MQ=255)
tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc > 1:2810377/1‑67 (MQ=255)
tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAgg > 1:3085803/1‑71 (MQ=255)
tttCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAgg > 1:3329839/1‑71 (MQ=255)
aGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt < 1:3500674/71‑1 (MQ=255)
aGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt < 1:350992/71‑1 (MQ=255)
gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:2759713/57‑1 (MQ=255)
gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:3857668/57‑1 (MQ=255)
gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:2079452/57‑1 (MQ=255)
cgcACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt > 1:255326/1‑69 (MQ=255)
gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag < 1:3785778/71‑1 (MQ=255)
gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag < 1:959456/71‑1 (MQ=255)
aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta < 1:3523494/71‑1 (MQ=255)
aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta < 1:2075116/71‑1 (MQ=255)
cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTaa < 1:2510110/48‑1 (MQ=255)
cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:3788474/56‑1 (MQ=255)
tGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:1979979/55‑1 (MQ=255)
gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGtt > 1:2145686/1‑71 (MQ=255)
gTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCa > 1:2363465/1‑56 (MQ=255)
gTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCa > 1:2598623/1‑56 (MQ=255)
cACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:1813313/1‑37 (MQ=255)
cACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:3744824/1‑37 (MQ=255)
aCGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATa < 1:3171134/71‑1 (MQ=255)
cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa < 1:335302/71‑1 (MQ=255)
aTTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag < 1:2624329/62‑1 (MQ=255)
aTTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag < 1:1726709/62‑1 (MQ=255)
tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:3378213/1‑56 (MQ=255)
tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:2245411/1‑56 (MQ=255)
tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:1247852/1‑56 (MQ=255)
tACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGtt > 1:3078735/1‑62 (MQ=255)
tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt > 1:1477368/1‑70 (MQ=255)
tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt > 1:260719/1‑70 (MQ=255)
catatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt < 1:55340/70‑1 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATc < 1:3071427/35‑1 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATc < 1:419773/35‑1 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCGAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:87917/64‑1 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:2458983/1‑71 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:2902148/1‑71 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:664345/1‑71 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:3060571/1‑71 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:2225784/64‑1 (MQ=255)
tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:1626926/64‑1 (MQ=255)
|
TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTGAT > W3110S.gb/3053020‑3053157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
