Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,488,502 |
C→T |
41.3% |
L47L (CTG→TTG) |
yijO → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,488,502 | 0 | C | T | 41.3%
| 24.7
/ 53.3
| 46 | L47L (CTG→TTG) | yijO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (6/21); new base T (9/10); total (15/31) |
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 |
CCAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTCC > W3110S.gb/3488437‑3488569
|
ccAGCAGTAACGGCCCGTTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGa < 1:1595587/70‑1 (MQ=255)
ccAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGa < 1:1692941/70‑1 (MQ=255)
aCGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTCAGttt > 1:1095830/1‑71 (MQ=255)
gCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:1441547/71‑1 (MQ=255)
tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:1980146/71‑1 (MQ=255)
tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:2609139/71‑1 (MQ=255)
tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:874319/71‑1 (MQ=255)
cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCgg < 1:2216586/71‑1 (MQ=255)
aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:214912/61‑1 (MQ=255)
aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:3503994/61‑1 (MQ=255)
aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:1582518/61‑1 (MQ=255)
cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:2539096/1‑70 (MQ=255)
cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGCAAGGTGAGTTTGTTGATACCGGCGCTg > 1:1470109/1‑70 (MQ=255)
cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:863216/1‑70 (MQ=255)
gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:1533269/54‑1 (MQ=255)
gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:914299/54‑1 (MQ=255)
gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:2901331/54‑1 (MQ=255)
cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCa < 1:2951246/71‑1 (MQ=255)
cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCa < 1:3762323/71‑1 (MQ=255)
gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:1392476/1‑54 (MQ=255)
gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:2910255/1‑54 (MQ=255)
tATCAGGTCGATTTTCCACGTCTGGAAATTGTGCTGGa < 1:2324352/38‑1 (MQ=255)
tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGaa < 1:1732881/37‑1 (MQ=255)
tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:1435568/1‑71 (MQ=255)
tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGttt < 1:3717328/46‑1 (MQ=255)
tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGttt < 1:1302706/46‑1 (MQ=255)
aGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:2318188/1‑69 (MQ=255)
ggTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAg < 1:1945673/71‑1 (MQ=255)
gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:217149/67‑1 (MQ=255)
gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:3377181/67‑1 (MQ=255)
gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:3381655/67‑1 (MQ=255)
gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:2084015/39‑1 (MQ=255)
gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:3581862/39‑1 (MQ=255)
cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:1236383/1‑45 (MQ=255)
cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:3400740/1‑45 (MQ=255)
cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:2384398/1‑45 (MQ=255)
cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:14291/1‑45 (MQ=255)
cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:94165/1‑71 (MQ=255)
tCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc < 1:2046589/70‑1 (MQ=255)
ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:3308628/70‑1 (MQ=255)
ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:2567763/70‑1 (MQ=255)
ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:2140709/70‑1 (MQ=255)
aCGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa < 1:3491534/36‑1 (MQ=255)
gCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgt > 1:462962/1‑69 (MQ=255)
gCTGGAAATTGTGTCGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg > 1:1700679/1‑70 (MQ=255)
tgCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTcc < 1:1809527/70‑1 (MQ=255)
|
CCAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTCC > W3110S.gb/3488437‑3488569
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
