Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,400,571 |
G→A |
14.6% |
L224L (CTG→TTG) |
fnr ← |
DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,400,571 | 0 | G | A | 14.6%
| 106.7
/ 10.1
| 41 | L224L (CTG→TTG) | fnr | DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base A (2/4); total (19/22) |
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
CGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA > W3110S.gb/1400503‑1400638
|
cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:981172/69‑1 (MQ=255)
cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:764486/69‑1 (MQ=255)
cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:667937/69‑1 (MQ=255)
cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:1017491/69‑1 (MQ=255)
cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:2681092/69‑1 (MQ=255)
aTGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACAt < 1:3425226/69‑1 (MQ=255)
tGACCAGCAAGCTGGGCCAGCTCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATg > 1:1322865/1‑69 (MQ=255)
tGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATg > 1:1973793/1‑69 (MQ=255)
gCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCtt < 1:2997922/69‑1 (MQ=255)
gggCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa < 1:3351645/69‑1 (MQ=255)
ccAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc < 1:902/69‑1 (MQ=255)
cAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCg < 1:2137654/69‑1 (MQ=255)
gcgtCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGAccc < 1:502737/66‑1 (MQ=255)
gcATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGAccc < 1:1122667/69‑1 (MQ=255)
cATCGTTATTTTCGATTGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa < 1:2305002/60‑1 (MQ=255)
tCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa > 1:2735165/1‑58 (MQ=255)
cGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca < 1:1019764/69‑1 (MQ=255)
cGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca < 1:82637/69‑1 (MQ=255)
gTTATTTTCGATGGTGATGTATTTACCTTTGATTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagcag > 1:195803/1‑69 (MQ=255)
gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc > 1:784977/1‑59 (MQ=255)
gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc < 1:3349043/59‑1 (MQ=255)
gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc > 1:2895715/1‑59 (MQ=255)
gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTCGAAGc > 1:2924672/1‑59 (MQ=255)
aTTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:2194248/1‑69 (MQ=255)
aTTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:3717395/1‑69 (MQ=255)
tCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCATCAGACGGCt < 1:2097478/68‑1 (MQ=255)
gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:1505165/1‑57 (MQ=255)
gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTg > 1:350076/1‑61 (MQ=255)
gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTg > 1:1182696/1‑61 (MQ=255)
aTGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCt < 1:2875293/69‑1 (MQ=255)
tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:1490445/49‑1 (MQ=255)
tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:1083683/49‑1 (MQ=255)
tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:2986731/1‑69 (MQ=255)
tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:2185550/1‑69 (MQ=255)
aTTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca > 1:48284/1‑48 (MQ=255)
aTTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:2634674/48‑1 (MQ=255)
tttACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:1783347/1‑67 (MQ=255)
tttACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:683864/69‑1 (MQ=255)
tACCTTTGACTGCCAGCATTCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:2291304/67‑1 (MQ=255)
tACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:96618/67‑1 (MQ=255)
tttGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGc < 1:1772259/68‑1 (MQ=255)
aCTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCa < 1:3010540/67‑1 (MQ=255)
tGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTATACCGTCAGGCCCAGAt < 1:73185/68‑1 (MQ=255)
gCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATa > 1:2612230/1‑68 (MQ=255)
cAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGtt > 1:1290542/1‑69 (MQ=255)
aGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTa > 1:2330617/1‑69 (MQ=255)
|
CGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA > W3110S.gb/1400503‑1400638
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
