Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,875,612 |
G→A |
28.6% |
D126N (GAT→AAT) |
iap → |
aminopeptidase in alkaline phosphatase isozyme conversion |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,875,612 | 0 | G | A | 28.6%
| 78.6
/ 33.8
| 49 | D126N (GAT→AAT) | iap | aminopeptidase in alkaline phosphatase isozyme conversion |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base A (6/8); total (23/26) |
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTA > W3110S.gb/2875544‑2875680
|
cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:2943336/69‑1 (MQ=255)
cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:529477/69‑1 (MQ=255)
cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:476449/69‑1 (MQ=255)
cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAAt > 1:558267/1‑69 (MQ=255)
cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAAt > 1:178671/1‑69 (MQ=255)
aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGAt < 1:1518897/68‑1 (MQ=255)
gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1690623/69‑1 (MQ=255)
gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:3179284/69‑1 (MQ=255)
gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1917766/69‑1 (MQ=255)
gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:2256365/69‑1 (MQ=255)
gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:2664622/69‑1 (MQ=255)
aaGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTg > 1:1880077/1‑69 (MQ=255)
gcgcCGCAGCAGATCATCATTATGGCGCATCTTGATACTTACGCCCCGCTGAGCGa < 1:3337308/56‑1 (MQ=255)
gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa < 1:3617497/56‑1 (MQ=255)
gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa < 1:3073045/56‑1 (MQ=255)
gccgcAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGc < 1:2523575/69‑1 (MQ=255)
gccgcAGCAGATCATCATTATGGCGAATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGc < 1:3595151/69‑1 (MQ=255)
atcatcATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTcggc < 1:3155090/69‑1 (MQ=255)
tcatcaTTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggcg > 1:363657/1‑69 (MQ=255)
catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1164571/46‑1 (MQ=255)
atcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt > 1:2703660/1‑45 (MQ=255)
catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAAtctc > 1:1808850/1‑61 (MQ=255)
catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt > 1:3415699/1‑69 (MQ=255)
catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt < 1:514859/69‑1 (MQ=255)
catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt < 1:725746/69‑1 (MQ=255)
catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt > 1:2011926/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:3008096/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:2833572/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:75249/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:667176/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:1738/1‑69 (MQ=255)
atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:1635746/1‑69 (MQ=255)
aTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAc > 1:617040/1‑68 (MQ=255)
aTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAc > 1:3360291/1‑68 (MQ=255)
tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:2900013/69‑1 (MQ=255)
tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg > 1:3303735/1‑69 (MQ=255)
tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:3450820/69‑1 (MQ=255)
tCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:36648/69‑1 (MQ=255)
tGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa > 1:243102/1‑69 (MQ=255)
tGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa > 1:1219580/1‑69 (MQ=255)
tACTTACGCCCCGCTGAGCGATGCTGACGCCGATTCCAATCTCGGCGGGCTGACGTTACAAGGAATg < 1:58640/67‑1 (MQ=255)
tACGCCGCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:2643932/69‑1 (MQ=255)
tACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:366503/69‑1 (MQ=255)
tACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:868447/69‑1 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGTCTGACGTTACAAGGAATGgat > 1:1435277/1‑64 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:165721/64‑1 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:2828305/64‑1 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:3460001/1‑64 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:3670687/1‑64 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:2824026/64‑1 (MQ=255)
cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:2834733/1‑64 (MQ=255)
cGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg < 1:937004/69‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGTGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:377312/69‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:540027/53‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:1747703/53‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:2803764/53‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:1043418/69‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:3637460/69‑1 (MQ=255)
gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:327702/69‑1 (MQ=255)
|
CTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTA > W3110S.gb/2875544‑2875680
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
