Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
491,553 |
A→G |
41.1% |
S74P (TCG→CCG) |
ybgS ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 491,553 | 0 | A | G | 41.1%
| 24.7
/ 52.2
| 73 | S74P (TCG→CCG) | ybgS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/31); new base G (12/18); total (24/49) |
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 |
TTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACATTTT > minE/491485‑491621
|
tttGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGa > 1:2054328/1‑69 (MQ=255)
tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:3118543/1‑69 (MQ=255)
tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:2145434/1‑69 (MQ=255)
tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:2113499/1‑69 (MQ=255)
tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc > 1:3108860/1‑68 (MQ=255)
tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc > 1:1658462/1‑68 (MQ=255)
ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:2603478/69‑1 (MQ=255)
ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:734273/69‑1 (MQ=255)
ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:2109600/69‑1 (MQ=255)
ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:762395/69‑1 (MQ=255)
gtgtTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAtt > 1:1536689/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGAt > 1:2734975/1‑48 (MQ=39)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTgcc > 1:3260901/1‑61 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:2286076/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:642777/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:446788/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1942571/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1686868/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1913039/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:508155/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:3238984/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:2589075/1‑69 (MQ=255)
ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1233973/1‑69 (MQ=255)
cATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCgg > 1:2531795/1‑69 (MQ=255)
cATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCgg > 1:2833525/1‑69 (MQ=255)
tCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt < 1:2905716/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt < 1:2765522/67‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2728220/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2686655/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:1355129/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:71393/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:36467/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:895279/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2439743/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2497568/68‑1 (MQ=255)
cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:1765737/68‑1 (MQ=255)
gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAtt < 1:2646603/65‑1 (MQ=255)
gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAtt < 1:1365132/65‑1 (MQ=255)
gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:967966/68‑1 (MQ=255)
ttCCGGCATTGTTCATTGAAGAACCATCCGAATGCAGCAGTGTGCCGCCAGACCCGGt < 1:261442/58‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3142893/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3144094/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3280036/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:1011490/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:2564687/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:531736/62‑1 (MQ=255)
ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:1523151/62‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCATAACCGGTATTTACCCCGt < 1:1680010/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2988470/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3315829/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3301363/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:935190/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:931244/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1231692/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1595711/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:737853/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3090178/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1775606/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1840546/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:547563/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2804408/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:28037/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:524764/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2708141/64‑1 (MQ=255)
tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2703405/64‑1 (MQ=255)
gTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGAc > 1:2573297/1‑69 (MQ=255)
tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:3330725/52‑1 (MQ=255)
tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:995023/52‑1 (MQ=255)
tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:2512587/52‑1 (MQ=255)
tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:1270871/52‑1 (MQ=255)
tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:3310366/52‑1 (MQ=255)
gaagaaCCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTAtt > 1:2805533/1‑68 (MQ=255)
gaagaaCCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTAtt > 1:2410083/1‑68 (MQ=255)
cGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAt > 1:763677/1‑68 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:703413/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1031397/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1116987/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1204100/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1205367/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1230036/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1666977/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1682905/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2133135/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:661910/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2422985/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:54688/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2476519/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2595640/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2983911/69‑1 (MQ=255)
aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:3194690/69‑1 (MQ=255)
|
TTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACATTTT > minE/491485‑491621
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
