Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
523,681 |
A→T |
41.3% |
R61S (AGA→AGT) |
moaA → |
molybdopterin biosynthesis protein A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 523,681 | 0 | A | T | 41.3%
| 21.2
/ 41.9
| 46 | R61S (AGA→AGT) | moaA | molybdopterin biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base A (12/15); new base T (9/10); total (21/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCG > minE/523627‑523742
|
tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGTCACCGaaaa < 1:3179033/69‑1 (MQ=255)
tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2304756/69‑1 (MQ=255)
tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:3081576/69‑1 (MQ=255)
tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:811758/69‑1 (MQ=255)
tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:3273726/69‑1 (MQ=255)
aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2721583/68‑1 (MQ=255)
aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2226454/68‑1 (MQ=255)
aaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:1302126/67‑1 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:948202/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:2000120/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1922552/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:398531/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:694937/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1638441/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1500313/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1498949/1‑68 (MQ=255)
ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1408562/1‑68 (MQ=255)
gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAATAGTGCGCCTGACaggag > 1:1759962/1‑67 (MQ=255)
gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAATAGTGCGCCTGACaggag > 1:1763868/1‑67 (MQ=255)
gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAg > 1:2744425/1‑52 (MQ=255)
gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACaggag > 1:746323/1‑67 (MQ=255)
gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACaggag > 1:2121168/1‑67 (MQ=255)
aaaTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGc > 1:2415635/1‑69 (MQ=255)
aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGTGGAGAGcc < 1:1074566/68‑1 (MQ=255)
aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:2623213/68‑1 (MQ=255)
aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:1990118/68‑1 (MQ=255)
aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:2244711/68‑1 (MQ=255)
cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:2755459/1‑68 (MQ=255)
cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:1666902/1‑68 (MQ=255)
cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:1676520/1‑68 (MQ=255)
cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:3342189/1‑68 (MQ=255)
cGCCGGGTTACGCGAGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:2177634/1‑68 (MQ=255)
cGCCGGATTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:7102/1‑68 (MQ=255)
ccGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCtt < 1:1697714/68‑1 (MQ=255)
ccGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGACGTCtt < 1:1748/68‑1 (MQ=255)
cGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCtt < 1:2122027/67‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTAcct < 1:47675/69‑2 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:481750/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:637208/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:686398/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:3387233/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:1798732/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:3198594/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:781625/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:2936322/69‑1 (MQ=255)
tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:1047594/69‑1 (MQ=255)
|
TAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCG > minE/523627‑523742
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
