Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
697,873 |
C→T |
28.1% |
R186R (CGC→CGT) |
appA → |
phosphoanhydride phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 697,873 | 0 | C | T | 28.1%
| 83.7
/ 31.2
| 78 | R186R (CGC→CGT) | appA | phosphoanhydride phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (42/14); new base T (15/7); total (57/21) |
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAG > minE/697808‑697933
|
gcgATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTcgcg < 1:1860416/67‑1 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGCCTTTACCGGGCATCGGCAAACGGCGTTTCGTGAGCTGGaa > 1:2617760/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGTGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1154129/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1009985/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3277844/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3248346/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1825873/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1738859/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3038045/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:2451728/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:552905/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:562904/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1562131/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:27981/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1477080/1‑69 (MQ=255)
cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:2728747/1‑69 (MQ=255)
agggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTg > 1:1562458/1‑69 (MQ=255)
agggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTg > 1:2737619/1‑69 (MQ=255)
gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:1635601/69‑1 (MQ=255)
gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2612187/69‑1 (MQ=255)
gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2546310/69‑1 (MQ=255)
gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:1525348/69‑1 (MQ=255)
gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:277904/69‑1 (MQ=255)
ggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2168122/68‑1 (MQ=255)
ggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2758656/68‑1 (MQ=255)
caggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTa > 1:1367855/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:1833067/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2015499/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2393372/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:182909/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:306764/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2248442/1‑69 (MQ=255)
aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:598148/1‑69 (MQ=255)
gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:1687096/1‑69 (MQ=255)
gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:229482/1‑69 (MQ=255)
gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:2268021/1‑69 (MQ=255)
gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:930564/1‑69 (MQ=255)
gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:2027414/1‑69 (MQ=255)
cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:2238390/1‑69 (MQ=255)
cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:2990173/1‑69 (MQ=255)
cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:664666/1‑69 (MQ=255)
cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:802059/1‑69 (MQ=255)
ggaaaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1954671/4‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:3026471/1‑65 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:2841262/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:545352/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3029739/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:462693/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3305688/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:864610/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3157124/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:108482/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1542124/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:161187/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1650674/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:16882/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1804185/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1876549/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1521531/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1486436/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1351451/1‑66 (MQ=255)
ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:224419/1‑66 (MQ=255)
aaaCGGCGTTTCTCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2462250/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2088903/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2173262/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2217432/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:389924/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:390542/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:394520/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:1681848/69‑1 (MQ=255)
aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:892061/69‑1 (MQ=255)
aaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:959743/68‑1 (MQ=255)
cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAAt > 1:2028109/1‑42 (MQ=255)
cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc > 1:3211205/1‑69 (MQ=255)
cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc > 1:1293687/1‑69 (MQ=255)
gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2157583/69‑1 (MQ=255)
gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2173438/69‑1 (MQ=255)
gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2650501/69‑1 (MQ=255)
tttCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2711023/61‑1 (MQ=255)
|
GCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAG > minE/697808‑697933
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
