Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 977,516 G→A 20.6% N543N (AAC→AAT)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE977,5160GA20.6% 221.9 / 41.0 149N543N (AAC→AAT) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base G (80/38);  new base A (21/10);  total (101/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 155 total aligned reads displayed.

AATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAAT  >  minE/977458‑977583
                                                          |                                                                   
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:1950478/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:1519660/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:2986757/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:2662009/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:187559/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:3242287/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:3354623/69‑3 (MQ=255)
aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTATTGAACAgac                                                           <  1:805121/69‑3 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:1984596/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:1130025/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:3348564/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:2137584/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:2042102/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:3170036/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:2654461/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:2119284/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:566525/1‑69 (MQ=255)
   gCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg                                                        >  1:207932/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGc                                                       >  1:453015/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGc                                                       >  1:1710027/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGc                                                       >  1:1476681/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGc                                                       >  1:487760/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGc                                                       >  1:3150144/1‑69 (MQ=255)
    ccAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGATGGc                                                       >  1:690704/1‑69 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2983953/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2922640/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2855824/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:3400765/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:794457/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:1267623/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2459495/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:1530517/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2334963/69‑1 (MQ=255)
      aGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt                                                     <  1:2196852/69‑1 (MQ=255)
            tatCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCa                                               <  1:1019444/69‑1 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          <  1:3305263/56‑1 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:3222082/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:2515075/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:335872/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:2516472/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:3092846/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:1026717/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:1806399/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          <  1:1349471/56‑1 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          <  1:1549215/56‑1 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          >  1:1602655/1‑56 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          <  1:1598574/56‑1 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAtt                                             >  1:1359952/1‑69 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAtt                                             >  1:3068581/1‑69 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAtt                                             >  1:28339/1‑69 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAt                                              >  1:410544/1‑68 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAt                                              >  1:2161474/1‑68 (MQ=255)
              tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAt                                              >  1:1090692/1‑68 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa                                                                >  1:1471307/1‑41 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGc                                                >  1:1173871/1‑57 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCa                                               >  1:2015705/1‑58 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCa                                               >  1:2319653/1‑58 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGc                                    >  1:348859/1‑69 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGc                                    >  1:1078988/1‑69 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGc                                    >  1:2914876/1‑69 (MQ=255)
                       gcggcgCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCCGGCATTACGCATTTTCTGTTGc                                    >  1:1577119/1‑69 (MQ=255)
                               aaGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCt                                                          <  1:2757077/39‑1 (MQ=255)
                                     ttCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:598469/69‑1 (MQ=255)
                                     ttCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:2072575/69‑1 (MQ=255)
                                     ttCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:3305782/69‑1 (MQ=255)
                                     ttCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:1152535/69‑1 (MQ=255)
                                     ttCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:444930/69‑1 (MQ=255)
                                       cGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAgg                         >  1:1587677/1‑64 (MQ=255)
                                       cGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAgg                         >  1:1251125/1‑64 (MQ=255)
                                       cGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAgg                         >  1:3237462/1‑64 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTGAGGCATtgc                   >  1:1060269/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:2295253/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:1101073/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:1336297/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:1255391/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:3201526/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:524421/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:596261/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:2340616/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:1533286/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtgc                   >  1:2750454/1‑69 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtg                    >  1:1760342/1‑68 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTCGTCAGGCATtg                    >  1:1756670/1‑68 (MQ=255)
                                        gCTCATCTCATACCCTTTATTGAACAGACTGGCATTACGCATTTTCTGTTGAAGTTAGTCAGGCATtgc                   >  1:1664437/1‑69 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCATTTCGTCAGGCATTGCTGCCCCGctct        >  1:2476270/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:2383925/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:771326/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:753034/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:394760/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:2690110/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:2577732/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:1769127/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctct        >  1:1070059/1‑68 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctc         >  1:824416/1‑67 (MQ=255)
                                                    cccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGctat        >  1:1101133/1‑66 (MQ=255)
                                                     ccTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTGGTCAGGCATTGCTGCCCCGCTCTg       >  1:1961856/1‑68 (MQ=255)
                                                         tgttgAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAAt  >  1:2585276/1‑69 (MQ=255)
                                                         tgttgAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAAt  >  1:65144/1‑69 (MQ=255)
                                                         tgttgAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAAt  >  1:1544772/1‑69 (MQ=255)
                                                          gttgAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCAt                      <  1:3172283/48‑1 (MQ=255)
                                                          |                                                                   
AATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTTTCTGTTGCAGTTCGTCAGGCATTGCTGCCCCGCTCTGATAAT  >  minE/977458‑977583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A