Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
994,518 |
T→C |
22.9% |
V131V (GTA→GTG) |
ynfK ← |
predicted dethiobiotin synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 994,518 | 0 | T | C | 22.9%
| 112.4
/ 26.9
| 87 | V131V (GTA→GTG) | ynfK | predicted dethiobiotin synthetase |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base C (8/12); total (39/48) |
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 |
AGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGA > minE/994453‑994585
|
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2825644/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:997871/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:3407663/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2633243/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2515559/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2490808/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2483918/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:483206/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2442205/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:411305/69‑1 (MQ=255)
aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2408520/69‑1 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:209746/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:1128018/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:3213035/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2406418/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:3154263/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2980486/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:290491/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:29077/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2003214/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:682839/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:1761244/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:531521/1‑68 (MQ=255)
gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACAGGCAGTTGTTCCTGCACTACCCa > 1:1835891/1‑68 (MQ=255)
ggTTAATGCAACCTTCCTGAATACCGACACCCATCAACACCGGCAGTTGTTCCTGCACTACCCAt > 1:265712/1‑65 (MQ=255)
ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:26890/1‑66 (MQ=255)
ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:1990178/1‑66 (MQ=255)
ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:1205478/1‑66 (MQ=255)
gTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCaga < 1:1194651/69‑1 (MQ=255)
aTACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:3331623/42‑1 (MQ=255)
gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:3029391/68‑1 (MQ=255)
gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:856344/68‑1 (MQ=255)
gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:53222/68‑1 (MQ=255)
gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:420709/68‑1 (MQ=255)
gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:2299297/68‑1 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCa > 1:2568230/1‑68 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCa > 1:2066386/1‑68 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:292822/1‑69 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2309264/1‑69 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2168533/1‑69 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2061862/1‑69 (MQ=255)
aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:3110280/1‑69 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:885758/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1172492/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:430748/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3116580/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:380011/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3249810/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3400536/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2813521/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1443740/69‑1 (MQ=255)
ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2184704/69‑1 (MQ=255)
ccGGCAGTGGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1781034/69‑1 (MQ=255)
cGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2720543/68‑1 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCa > 1:1229926/1‑68 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2796535/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:969335/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2735370/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:888375/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2431597/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2211601/1‑69 (MQ=255)
gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:1322687/1‑69 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:1481723/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1218510/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1479079/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:568410/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:643021/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:693465/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1236752/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:979975/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:166063/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:2986783/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:243843/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3179276/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3324458/68‑1 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:2210646/1‑68 (MQ=255)
gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3402971/68‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1306719/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3300040/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:822771/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:852809/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:2291082/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:86436/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1804998/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3017406/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3010130/69‑1 (MQ=255)
cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1561544/69‑1 (MQ=255)
tACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:772377/68‑1 (MQ=255)
|
AGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGA > minE/994453‑994585
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
