Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,053,053 |
G→A |
22.7% |
R91C (CGT→TGT) |
ydhF ← |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,053,053 | 0 | G | A | 22.7%
| 205.7
/ 48.2
| 149 | R91C (CGT→TGT) | ydhF | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (84/31); new base A (5/29); total (89/60) |
| Fisher's exact test for biased strand distribution p-value = 2.14e-09 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
Only 100 of 149 total aligned reads displayed.
GCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGT > minE/1052997‑1053117
|
ctctTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTcgc > 1:452983/1‑68 (MQ=255)
ttAATGATGTGATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATa > 1:1713200/1‑69 (MQ=255)
ttAATGATGTGATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATa > 1:760989/1‑69 (MQ=255)
aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATa > 1:1595667/1‑67 (MQ=255)
aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc > 1:1873947/1‑68 (MQ=255)
aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc > 1:717263/1‑68 (MQ=255)
aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc > 1:387119/1‑68 (MQ=255)
tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc < 1:2078292/66‑1 (MQ=255)
tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc < 1:2570627/66‑1 (MQ=255)
tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc < 1:2397752/66‑1 (MQ=255)
tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc < 1:449774/66‑1 (MQ=255)
gatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc < 1:1103229/65‑1 (MQ=255)
aagtgATCGCGGTCAGTGATGTAATGCCCAATGACGTTTTCTTCACGCGCGGtcgtcg > 1:1021025/3‑58 (MQ=39)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATAGCGCAtt < 1:435203/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt < 1:774895/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt < 1:931198/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt < 1:195949/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt < 1:309659/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTAACACGCGGTCGTCGCGATACCGCAtt < 1:2378460/68‑1 (MQ=255)
gtgATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCAtt < 1:2144479/68‑1 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGa > 1:2331694/1‑56 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:476786/1‑69 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:428947/1‑69 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:796704/1‑69 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:2710832/1‑69 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:1754388/1‑69 (MQ=255)
aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt > 1:3271501/1‑69 (MQ=255)
cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg > 1:2902793/1‑68 (MQ=255)
cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg > 1:1741117/1‑68 (MQ=255)
cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg > 1:3224290/1‑68 (MQ=255)
cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTGACTg > 1:3348294/1‑68 (MQ=255)
aGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGa > 1:2440893/1‑47 (MQ=255)
aGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGAt > 1:3422616/1‑48 (MQ=255)
tGATGTAATGGCCTATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGAAGATTTcc < 1:636031/68‑1 (MQ=255)
tGATGTAATGGCCAATGGCGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:1420764/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:308302/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:3035417/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:3224021/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2652178/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2574854/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2482506/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2460304/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:1822734/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2022424/68‑1 (MQ=255)
tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:2028035/68‑1 (MQ=255)
aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc > 1:1716253/1‑69 (MQ=255)
aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc > 1:341679/1‑69 (MQ=255)
aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc > 1:511908/1‑69 (MQ=255)
tGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:1062668/65‑1 (MQ=255)
tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCTCGATACCGCATTTACTGACGATTTcc < 1:292422/63‑1 (MQ=255)
tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:1640393/63‑1 (MQ=255)
tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:313364/63‑1 (MQ=255)
tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:3016962/63‑1 (MQ=255)
tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:1209053/63‑1 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa > 1:1392881/1‑62 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa > 1:1736128/1‑62 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa > 1:1465863/1‑62 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa > 1:2598755/1‑62 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt > 1:2717919/1‑68 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt > 1:249215/1‑68 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt > 1:2648245/1‑68 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt > 1:2889223/1‑68 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:3004346/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:384814/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:276905/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:459052/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:3378075/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:2839443/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:2578678/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:219359/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:707604/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:2660021/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc > 1:2943611/1‑69 (MQ=255)
aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGAATTCCATCCGTTc > 1:538572/1‑69 (MQ=255)
ccAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc < 1:944115/57‑1 (MQ=255)
gACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtg > 1:1115647/1‑69 (MQ=255)
gACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtg > 1:2116472/1‑69 (MQ=255)
ttttCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggc < 1:937821/69‑2 (MQ=255)
ttttCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggc < 1:2341983/69‑2 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:1539704/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:2444563/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:1597757/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:2589091/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:2780411/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:3029492/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:2005561/1‑67 (MQ=255)
ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt > 1:3395553/1‑67 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:1854552/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:3145658/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:609320/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:1464097/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:2794468/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:1361388/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:2287385/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:2283565/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:832236/68‑1 (MQ=255)
cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc < 1:1020589/68‑1 (MQ=255)
cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt > 1:985761/1‑68 (MQ=255)
cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt > 1:1518497/1‑68 (MQ=255)
cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt > 1:1653618/1‑68 (MQ=255)
|
GCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGT > minE/1052997‑1053117
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
