Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,066,835 |
C→G |
42.2% |
V219L (GTT→CTT) |
ydhB ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,066,835 | 0 | C | G | 42.2%
| 36.7
/ 84.4
| 83 | V219L (GTT→CTT) | ydhB | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (42/6); new base G (21/14); total (63/20) |
| Fisher's exact test for biased strand distribution p-value = 8.25e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 |
ATCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCGCGC > minE/1066787‑1066899
|
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTAt > 1:242289/1‑63 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1161682/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:978293/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:731875/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:343399/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:3303098/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2970478/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2384896/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2326170/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:23041/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2133012/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1933542/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1895111/1‑69 (MQ=255)
aTCCTGCCGAGATACAGGTTACCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:433271/1‑69 (MQ=255)
ccGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:3021249/69‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1082630/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:2998814/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1265069/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:124510/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:152107/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:607244/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:642831/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:691434/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:2069861/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:803423/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1906275/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1927246/67‑1 (MQ=255)
gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:87336/67‑1 (MQ=255)
agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:1979081/69‑1 (MQ=255)
agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:2418623/69‑1 (MQ=255)
agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:3069031/69‑1 (MQ=255)
cAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACg > 1:3346171/1‑68 (MQ=255)
gatgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAAc < 1:9658/48‑1 (MQ=255)
atgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCg < 1:2198207/69‑1 (MQ=255)
atgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCg < 1:1397697/69‑1 (MQ=255)
gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:177743/1‑36 (MQ=255)
gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:849466/1‑36 (MQ=255)
gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:3408426/1‑36 (MQ=255)
gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGtt > 1:2350869/1‑69 (MQ=255)
gatTACCAGTCAGGCACGACAACTCTTTTTTGGtt > 1:2468504/1‑35 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1601676/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:954551/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:137553/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:943445/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:419793/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:449595/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:586422/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:808310/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:804311/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2963494/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2905055/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2822710/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1592164/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1560874/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1896302/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2324995/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:14817/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2338369/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2348122/1‑67 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcg > 1:2936496/1‑66 (MQ=255)
ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGAGTTcgc > 1:2446048/1‑67 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:161350/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:3279803/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:943532/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:564726/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:1752870/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:3119311/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:2162053/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:738034/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTCAGGTAGCGTTCGCGAGgtg > 1:2587455/1‑69 (MQ=255)
tCAGGCACGACAACTCTTTTTTGGTTAGCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:1759356/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1184706/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1551653/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1553907/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:3131646/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2222861/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:810312/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2248189/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1675509/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:464417/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1641772/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1452828/1‑69 (MQ=255)
aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2368649/1‑69 (MQ=255)
|
ATCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCGCGC > minE/1066787‑1066899
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
