Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,897,196 T→C 30.9% I306I (ATT→ATC)  argA → fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,897,1960TC30.9% 116.9 / 64.8 129I306I (ATT→ATC) argAfused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (18/71);  new base C (13/27);  total (31/98)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

GGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCG  >  minE/1897131‑1897260
                                                                 |                                                                
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2597848/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:1473/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:1709628/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:1732358/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:1771095/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:642859/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2149952/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2161967/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2637127/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:361383/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2694467/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:89039/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:2734680/69‑1 (MQ=255)
  tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg                                                             <  1:323133/69‑1 (MQ=255)
    tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa                                                           <  1:3351998/69‑1 (MQ=255)
    tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa                                                           <  1:1847057/69‑1 (MQ=255)
    tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa                                                           <  1:2770200/69‑1 (MQ=255)
    tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa                                                           <  1:2986510/69‑1 (MQ=255)
      ggTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTAt                                                          <  1:1216850/68‑1 (MQ=255)
      ggTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTAt                                                          <  1:2086323/68‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:1163911/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:1317320/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:2530197/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               >  1:1615385/1‑69 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:877881/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:2875934/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:843938/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:2623358/69‑1 (MQ=255)
                ttGTGATGGAAAGCGCAGATCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:1518041/69‑1 (MQ=255)
                 tgtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:1509382/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:217243/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:278774/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:443149/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:477743/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:687927/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:1065384/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:1678972/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc                                            <  1:753104/68‑1 (MQ=255)
                    gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGAAGTTGATTc                                            <  1:3421936/68‑1 (MQ=255)
                     aTGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCg                                           >  1:2119461/1‑68 (MQ=255)
                        gAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCa                                       >  1:800519/1‑69 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:2668550/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:2763032/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:1461671/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:2206488/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:862214/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:818673/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:1479170/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:3184359/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:1345687/69‑1 (MQ=255)
                               ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:1347279/69‑1 (MQ=255)
                                cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:2259412/68‑1 (MQ=255)
                                cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:91300/68‑1 (MQ=255)
                                 gAGCAGATTCGTCGCGCAACAATCATCGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc                                <  1:2484970/67‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa                                               <  1:2063987/47‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:1873488/69‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:73782/69‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:351389/69‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:1200757/69‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:1245847/69‑1 (MQ=255)
                                      gATTCGTCGCGCAACAATCAACGATATTGGCGGAATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                         <  1:3142079/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAAGGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGt                        >  1:2788910/1‑68 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       >  1:3378884/1‑69 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       >  1:558509/1‑69 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:824599/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       >  1:3179443/1‑69 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:925718/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:936442/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:1767693/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:1854208/69‑1 (MQ=255)
                                        ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                       <  1:1190648/69‑1 (MQ=255)
                                              gcgcAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTgg                 <  1:2739634/69‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:1453242/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:1466533/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:160171/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:1451846/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:3040000/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:1221564/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:1120087/60‑1 (MQ=255)
                                                     aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt                   <  1:2847593/60‑1 (MQ=255)
                                                      aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt         <  1:115095/69‑1 (MQ=255)
                                                      aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt         <  1:2681626/69‑1 (MQ=255)
                                                      aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt         <  1:1603264/69‑1 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc     >  1:275683/1‑68 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc     >  1:3372361/1‑68 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc     >  1:2463467/1‑68 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc     >  1:3315917/1‑68 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc     >  1:2938241/1‑68 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct      >  1:3368779/1‑67 (MQ=255)
                                                           cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct      >  1:1029741/1‑67 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGt                >  1:1718001/1‑55 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:1011712/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:2076207/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:675606/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:2455724/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:2773093/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:3376157/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:2790564/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:2838834/1‑69 (MQ=255)
                                                             atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg  >  1:3038475/1‑69 (MQ=255)
                                                                 |                                                                
GGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCG  >  minE/1897131‑1897260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A