Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,614 |
A→G |
55.7% |
D287D (GAT→GAC) |
prfB ← |
peptide chain release factor RF‑2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,614 | 0 | A | G | 55.7%
| 20.6
/ 70.1
| 88 | D287D (GAT→GAC) | prfB | peptide chain release factor RF‑2 |
| Reads supporting (aligned to +/- strand): ref base A (16/23); new base G (32/17); total (48/40) |
| Fisher's exact test for biased strand distribution p-value = 3.14e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 |
TCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934546‑1934676
|
tCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa < 1:1186300/69‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:2505047/65‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:1984329/65‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3150433/65‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:501700/65‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3328264/65‑1 (MQ=255)
aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:2453742/65‑1 (MQ=255)
gAGTCATCAAGGACATAAGAACGTATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:143685/69‑1 (MQ=255)
gAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:1468851/69‑1 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCCGCCGATGTCGGATTTGTTATCTTcc > 1:2752585/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATTTCGGATTTATTGTCTTCCATCGCCTGttt > 1:829365/1‑69 (MQ=38)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2516551/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2356865/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:1159194/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:986743/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2012054/1‑58 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2346226/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2167385/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:247824/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3178220/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2516617/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2517886/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2775622/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:1014821/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3213361/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3241470/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:436059/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:546748/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:61427/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:989974/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:147928/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:796446/1‑68 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:600255/1‑68 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCATCCATCGCCTGttt > 1:2701666/1‑69 (MQ=255)
atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCAGATGTCGGATTTGTTATCTTcc > 1:3149678/1‑58 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:2603170/1‑49 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:3399203/1‑49 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:397533/1‑49 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:1206551/1‑49 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:178429/1‑69 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:3127816/1‑69 (MQ=255)
aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:2070329/1‑69 (MQ=255)
ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:2781954/69‑1 (MQ=255)
ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:1507202/69‑1 (MQ=255)
ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:854292/69‑1 (MQ=255)
aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2219956/1‑69 (MQ=255)
aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:3337018/1‑69 (MQ=255)
aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2691763/1‑69 (MQ=255)
aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:926240/69‑1 (MQ=255)
aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:1672037/69‑1 (MQ=255)
gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2306254/69‑1 (MQ=255)
gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2828619/69‑1 (MQ=255)
gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:1947593/69‑1 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtctc > 1:1817393/1‑37 (MQ=18)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtct > 1:1087597/1‑36 (MQ=18)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCTGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1224988/1‑69 (MQ=38)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1111457/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:3259329/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1249445/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:784018/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2868757/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:74227/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:997813/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2223796/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2293531/1‑69 (MQ=255)
cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1884385/1‑69 (MQ=255)
tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:2751105/1‑69 (MQ=255)
tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:443924/1‑69 (MQ=255)
ggATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2253456/1‑39 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATGTCCAGTTCATATAGCTTCGCtt < 1:2531440/69‑1 (MQ=38)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1871262/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2084547/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2208936/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2284600/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:629759/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1156135/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1161119/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:178065/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1240247/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1629754/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1324707/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2755036/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:3173556/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2779254/69‑1 (MQ=255)
aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:3129970/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:2472880/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:310302/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1871763/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1762128/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:855392/69‑1 (MQ=255)
tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:2117296/69‑1 (MQ=255)
tttATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1000157/68‑1 (MQ=255)
ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1487685/68‑1 (MQ=255)
|
TCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934546‑1934676
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
