Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,006,358 |
C→T |
57.7% |
G189G (GGG→GGA) |
hybB ← |
predicted hydrogenase 2 cytochrome b type component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,006,358 | 0 | C | T | 57.7%
| 24.9
/ 63.7
| 85 | G189G (GGG→GGA) | hybB | predicted hydrogenase 2 cytochrome b type component |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base T (35/14); total (51/34) |
| Fisher's exact test for biased strand distribution p-value = 1.48e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 |
CAGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACC > minE/2006292‑2006421
|
cAGCGGCAACGTTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1931734/69‑1 (MQ=255)
cAGCGGCAACATTTCATAGCTCTGTCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1664553/69‑1 (MQ=255)
aGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1130094/68‑1 (MQ=255)
aGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:3010105/68‑1 (MQ=255)
aaCATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGaa < 1:431581/69‑1 (MQ=255)
aaCATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGaa < 1:1358944/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGTTCATTAGCGACCCCATTGAAGACTgg < 1:942344/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2921011/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:229705/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2833842/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:1251173/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:941404/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:982879/69‑1 (MQ=255)
tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2571759/69‑1 (MQ=255)
cATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:1427328/68‑1 (MQ=255)
aTGCACCTTGTAGCCCGCCTAGATCATCAGCGATCCCa > 1:2794896/1‑38 (MQ=38)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGAtccc > 1:769297/1‑37 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2185736/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1787661/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1835167/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:416548/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:404664/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:401917/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:3220663/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2722577/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2585026/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1109485/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:679001/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1487700/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:689878/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1288531/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1294363/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1393433/1‑38 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:1707761/69‑1 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:2753730/69‑1 (MQ=255)
gCCCGCCTAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCcat > 1:1863398/1‑69 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:2961699/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1045145/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1345899/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1616519/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1644915/69‑1 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGgcag > 1:1777331/1‑41 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2298012/1‑68 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2952348/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:899179/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2911097/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2966846/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:80504/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:584893/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1095666/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1138461/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1734151/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1445248/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1441565/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2267352/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2208772/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1360952/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1985450/1‑69 (MQ=255)
ataatcaGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2473503/4‑68 (MQ=255)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3192870/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:157013/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:477388/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:790864/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:795274/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3349989/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:80399/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:8186/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3215600/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3074979/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2018400/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2300039/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2901254/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:287622/37‑1 (MQ=37)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc < 1:402178/69‑1 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1226388/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1193713/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1510496/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:596133/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:591350/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1836348/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1918847/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2595053/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:197059/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:328097/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2160748/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2323968/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2506644/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:258815/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAc > 1:3317296/1‑68 (MQ=255)
gCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATtaagaa < 1:1412031/61‑1 (MQ=255)
|
CAGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACC > minE/2006292‑2006421
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
