Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,070,836	G→A	31.9%	V219V (GTG→GTA)	ygjG →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,070,836	0	G	A	31.9%	44.6 / 23.7	53	V219V (GTG→GTA)	ygjG	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base G (5/31); new base A (6/11); total (11/42)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGA  >  minE/2070779‑2070898

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:84539/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:2737541/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:2913807/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:1777287/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:1649182/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:319116/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:58380/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:835846/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc                                                       <  1:1188394/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATCTACCGTTTGGc                                                       <  1:3149774/67‑1 (MQ=255)

ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGCCATGTACCGTTTGGc                                                       <  1:835924/67‑1 (MQ=255)

 gCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAat                                                    <  1:2492461/69‑1 (MQ=255)

                           tATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAat                                                    <  1:3265012/43‑1 (MQ=255)

                              gCCGTTACTGCCGGGCTTCCGTCATGTGCCTTTTGGCAATATCGAAGc                                            <  1:428991/48‑1 (MQ=255)

                                    aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa                  >  1:2227414/1‑68 (MQ=255)

                                    aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa                  >  1:1424714/1‑68 (MQ=255)

                                    aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa                  >  1:129595/1‑68 (MQ=255)

                                    aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa                  >  1:1655725/1‑68 (MQ=255)

                                    aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa                  >  1:3134690/1‑68 (MQ=255)

                                          gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa             <  1:1215032/67‑1 (MQ=255)

                                          gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa             <  1:2679208/67‑1 (MQ=255)

                                          gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa             <  1:2758346/67‑1 (MQ=255)

                                          gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa             <  1:3027795/67‑1 (MQ=255)

                                             cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg                        <  1:2314750/53‑1 (MQ=255)

                                             cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg                        <  1:479324/53‑1 (MQ=255)

                                             cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg                        <  1:1060656/53‑1 (MQ=255)

                                             cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg                        <  1:214828/53‑1 (MQ=255)

                                             cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg                        <  1:1781301/53‑1 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:2145945/2‑51 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:1297477/2‑51 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:2059532/2‑51 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:2928934/2‑51 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:409526/2‑51 (MQ=255)

                                                tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa                       >  1:380957/2‑51 (MQ=255)

                                                  gTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgat    <  1:1373926/68‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1585615/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:664557/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1334454/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:529832/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1411514/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:411499/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1416802/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:3245557/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:2199247/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1595535/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:3072550/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1805537/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1999101/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:2145900/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1015234/69‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatg   <  1:3135181/68‑1 (MQ=255)

                                                   tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatg   <  1:3131352/68‑1 (MQ=255)

                                                    cATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga  <  1:1277115/68‑1 (MQ=255)

GGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGA  >  minE/2070779‑2070898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A