Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,108,982 G→T 49.9% P251P (CCG→CCT)  kbaY → tagatose 6‑phosphate aldolase 1, kbaY subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,108,9820GT49.9% 13.8 / 116.2 104P251P (CCG→CCT) kbaYtagatose 6‑phosphate aldolase 1, kbaY subunit
Reads supporting (aligned to +/- strand):  ref base G (31/21);  new base T (6/46);  total (37/67)
Fisher's exact test for biased strand distribution p-value = 4.15e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTC  >  minE/2108920‑2109042
                                                              |                                                            
gTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGt                                                        <  1:2167033/69‑1 (MQ=255)
gTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGt                                                        <  1:1383827/69‑1 (MQ=255)
                 aaTAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1823761/69‑1 (MQ=255)
                 aaTAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1151201/69‑1 (MQ=255)
                 aaTAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatt                                        <  1:353757/68‑1 (MQ=255)
                   tAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1919577/67‑1 (MQ=255)
                   tAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1904183/67‑1 (MQ=255)
                   tAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1727389/67‑1 (MQ=255)
                   tAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:3023885/67‑1 (MQ=255)
                   tAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:1202131/67‑1 (MQ=255)
                   tAGCCTTCGCTGGAGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatta                                       <  1:2672652/67‑1 (MQ=255)
                       cTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATgcg                                 <  1:35196/69‑1 (MQ=255)
                             tgtcgcgGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           <  1:83767/66‑1 (MQ=255)
                             tGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           <  1:2431622/69‑1 (MQ=255)
                             tGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           <  1:1665532/69‑1 (MQ=255)
                                cgcgGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           <  1:3004808/66‑1 (MQ=255)
                                 gcgGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       <  1:2416833/69‑1 (MQ=255)
                                   ggTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGa                     <  1:2152313/69‑1 (MQ=255)
                                   ggTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGa                     <  1:2162440/69‑1 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTtattat                                      >  1:619676/1‑51 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGAt                    >  1:1330693/1‑69 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGAt                    >  1:2875257/1‑69 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGAt                    >  1:1112081/1‑69 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGAt                    >  1:39300/1‑69 (MQ=255)
                                    gTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGAt                    >  1:617732/1‑69 (MQ=255)
                                      tAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttat                                         >  1:2106714/1‑46 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:177024/1‑68 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:177330/1‑68 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:755077/1‑68 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:2380386/1‑68 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:2737980/1‑68 (MQ=255)
                                          gCCTGGTTTGCGGAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAta              >  1:1083387/1‑68 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                                            >  1:461614/1‑38 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                                            >  1:2382967/1‑38 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                                            >  1:1070818/1‑38 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           >  1:91330/1‑55 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:2211745/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:2288938/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:1931391/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:27346/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:1798752/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:1704447/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:1679998/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:3160527/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:1489366/1‑59 (MQ=255)
                                           ccTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATg                       >  1:875717/1‑59 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:2603678/1‑66 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:945724/1‑66 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:1022633/1‑66 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:950605/1‑66 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:225504/1‑66 (MQ=255)
                                            cTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGAt               >  1:2160108/1‑66 (MQ=255)
                                             tGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGaa            >  1:1110088/1‑68 (MQ=255)
                                             tGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGaa            >  1:2516219/1‑68 (MQ=255)
                                             tGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGaa            >  1:2576264/1‑68 (MQ=255)
                                                      gAAATTCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:1152323/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATTGATGCGATGAAAGAAGTTGTc  <  1:2521243/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:318944/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1085614/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:3310405/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:3366364/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:347525/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1554055/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:155243/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1397992/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:595298/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:136099/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1340088/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1284350/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:818087/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:82875/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:117562/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:844247/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1166103/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:16197/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:2485421/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1652534/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:2052848/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGt   <  1:1895656/68‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2355045/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:3162524/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2232965/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2417992/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:213457/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2601076/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2007662/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:1870307/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:275516/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2771724/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:557151/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:1755775/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:1402844/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:2912838/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:3429951/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:3415475/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:1636010/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:331646/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:3149285/69‑1 (MQ=255)
                                                      gAAAATCCTCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTc  <  1:3192298/69‑1 (MQ=255)
                                                           tCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg                           <  1:2996472/39‑1 (MQ=255)
                                                              |                                                            
GTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGGAATGGATGCGATGAAAGAAGTTGTC  >  minE/2108920‑2109042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A