Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,156,251 A→G 38.8% G199G (GGT→GGC)  obgE ← GTPase involved in cell partioning and DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,156,2510AG38.8% 39.8 / 57.2 86G199G (GGT→GGC) obgEGTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base A (16/36);  new base G (5/28);  total (22/64)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

CTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAT  >  minE/2156190‑2156311
                                                             |                                                            
cTTCAATCAGTCCTGGAATATCGGCAACAACTAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:907634/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:989352/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2635984/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1021031/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2547155/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:274520/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2856289/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2899295/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2993030/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2327630/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:231191/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:3094189/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:2210344/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:3155699/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:3345559/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1792839/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:865809/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1384228/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:676571/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1453395/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:630354/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:819694/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1615736/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:872768/69‑3 (MQ=255)
cTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1705367/69‑3 (MQ=255)
 ttCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:3273671/68‑3 (MQ=255)
 ttCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:1889374/68‑3 (MQ=255)
   cAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACtt                                                     >  1:2142843/1‑68 (MQ=255)
   cAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACtt                                                     >  1:356865/1‑68 (MQ=255)
   cAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACtt                                                     >  1:2265770/1‑68 (MQ=255)
   cAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACtt                                                     >  1:2602573/1‑68 (MQ=255)
       cAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATACGCACCACGCCCAggc                                                       <  1:155456/62‑3 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:305077/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:911468/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:2727922/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:303559/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:3133292/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:611404/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:545682/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:3374726/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:3379263/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:1244361/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:26053/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:1785942/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:1387395/59‑1 (MQ=255)
        aGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:1337753/59‑1 (MQ=255)
         gTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAg                                                         <  1:917025/58‑1 (MQ=255)
           ccTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCaga                                            >  1:1639483/1‑69 (MQ=255)
           ccTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCaga                                            >  1:816121/1‑69 (MQ=255)
           ccTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCaga                                            >  1:958964/1‑69 (MQ=255)
           ccTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCaga                                            >  1:25460/1‑69 (MQ=255)
                    tCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACCCCCAGACTTGGCACCAGAGTGGTaac                                    >  1:2602486/1‑67 (MQ=255)
                    tCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTaaa                                    >  1:2464763/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:663575/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:1693489/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:48575/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:418331/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:417807/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:3432368/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:2384159/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:1936310/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACgg                                 <  1:2237736/69‑1 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:1975082/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:2835908/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:691022/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:403732/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:2122576/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACg                                  >  1:1492238/1‑68 (MQ=255)
                      ggCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACAAGAGTGGTAAAc                                   >  1:2841793/1‑67 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:799166/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:1277059/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:568277/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:420399/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:1917043/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:3248516/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:3181769/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:2204772/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:2218965/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:3031280/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:2432815/68‑1 (MQ=255)
                                                   ttCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgcc     <  1:1066648/68‑1 (MQ=255)
                                                     cgcaCCACGCCCAGGCTTGGTACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt  >  1:1738991/4‑69 (MQ=255)
                                                     cgcaCCACGCCCAGGCTTGGTACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt  >  1:1328421/4‑69 (MQ=255)
                                                     cgcaCCACGCCCAGGCTTGGTACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt  >  1:1361819/4‑69 (MQ=255)
                                                     cgcaCCACGCCCAGGCTTGGTACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt  >  1:231164/4‑69 (MQ=255)
                                                     cgcaCCACGCCCAGGCTTGGTACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt  >  1:2652193/4‑69 (MQ=255)
                                                             |                                                            
CTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAT  >  minE/2156190‑2156311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A