Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,423,180 |
A→G |
31.2% |
V96V (GTT→GTC) |
rffA ← |
TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,423,180 | 0 | A | G | 31.2%
| 100.1
/ 58.0
| 115 | V96V (GTT→GTC) | rffA | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Reads supporting (aligned to +/- strand): ref base A (19/60); new base G (14/22); total (33/82) |
| Fisher's exact test for biased strand distribution p-value = 1.22e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 138 total aligned reads displayed.
TCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATC > minE/2423115‑2423246
|
tCGGTGATCGCCGCTTCTATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2037309/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:485679/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:3274193/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2191329/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:3154617/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2434957/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1289215/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2764571/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:53998/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2905107/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1866077/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:190561/68‑1 (MQ=255)
tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:110037/68‑1 (MQ=255)
tCGGGGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1920828/68‑1 (MQ=255)
cGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTACGGGCGAACATCCACAAAGAc < 1:1056429/67‑1 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:3155446/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:2943531/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:1669744/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:2344830/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:111385/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:236292/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:745179/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:475280/1‑68 (MQ=255)
aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAtttt > 1:2216038/1‑68 (MQ=255)
cgccgcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACGAAAACGATTTTTgc > 1:3004382/1‑69 (MQ=255)
cgccgcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTgc > 1:1390094/1‑69 (MQ=255)
gcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCa < 1:1558812/69‑1 (MQ=255)
aTCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACgca < 1:1645098/67‑1 (MQ=255)
aTCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACgca < 1:1230736/67‑1 (MQ=255)
tttCGTCTATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1282096/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2523802/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:264349/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2794493/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2264360/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2458031/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:3080815/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2820695/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1870891/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1789903/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1749461/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1576930/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1532289/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:3340795/69‑1 (MQ=255)
tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1085662/69‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2920749/68‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:891804/68‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAATGc < 1:1305593/69‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:2362752/69‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:545416/69‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:968028/69‑1 (MQ=255)
ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:1639231/69‑1 (MQ=255)
tcgtcgATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:1179264/68‑1 (MQ=255)
gATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1680524/36‑1 (MQ=255)
aTGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2144308/35‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1487431/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2373493/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2261204/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:362586/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1112546/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2128332/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1490891/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1170720/56‑1 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:544029/1‑69 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:3221971/1‑69 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:1694425/1‑69 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:717246/1‑69 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:2720320/1‑69 (MQ=255)
cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:2456518/1‑69 (MQ=255)
gtgtCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGgaga > 1:3052311/1‑67 (MQ=255)
cGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2943744/64‑1 (MQ=255)
cGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2063147/64‑1 (MQ=255)
ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:450771/68‑1 (MQ=255)
ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:2828139/68‑1 (MQ=255)
ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:893653/68‑1 (MQ=255)
gCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:434659/67‑1 (MQ=255)
cATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2516211/56‑1 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTg > 1:987502/1‑40 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1449194/1‑69 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:74441/1‑69 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1129222/1‑69 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1798801/1‑69 (MQ=255)
cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1209665/1‑69 (MQ=255)
caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:3427535/68‑1 (MQ=255)
caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:232948/68‑1 (MQ=255)
caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:447913/68‑1 (MQ=255)
caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:2891119/68‑1 (MQ=255)
ataaaCGATTTTTGCGCCACGAAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:2083252/65‑1 (MQ=255)
aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:3263417/69‑1 (MQ=255)
aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:1370307/69‑1 (MQ=255)
aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:1985187/69‑1 (MQ=255)
aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:2292792/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1230708/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:662782/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:666800/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:394027/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:354024/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1751545/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:806606/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1772359/69‑1 (MQ=255)
aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:2253164/69‑1 (MQ=255)
|
TCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATC > minE/2423115‑2423246
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
