Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,464,552 |
T→C |
100% |
G70G (GGT→GGC) |
atpB → |
F0 sector of membrane‑bound ATP synthase, subunit a |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,464,552 | 0 | T | C | 75.2%
| 88.9
/ 22.6
| 72 | G70G (GGT→GGC) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
| Reads supporting (aligned to +/- strand): ref base T (1/17); new base C (6/48); total (7/65) |
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAC > minE/2464489‑2464618
|
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1951186/1‑69 (MQ=255)
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:2913635/1‑69 (MQ=255)
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1051871/1‑69 (MQ=255)
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1236865/1‑69 (MQ=255)
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:912934/1‑69 (MQ=255)
ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGCCGTGcc > 1:2631172/1‑69 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:465947/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:19419/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2039268/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2251824/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2307074/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2380945/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2586080/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3308366/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2821417/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:417408/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3137957/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:689547/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:1142123/67‑1 (MQ=255)
tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:71188/67‑1 (MQ=255)
tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:3401413/68‑1 (MQ=255)
tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:1633615/68‑1 (MQ=255)
ttATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTg > 1:1884471/1‑69 (MQ=255)
agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:2626123/69‑1 (MQ=255)
agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:402823/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:1591593/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3308337/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:589068/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2742253/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3281415/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:909482/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:452975/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2898666/69‑1 (MQ=255)
gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGCAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:803635/69‑1 (MQ=255)
gtagcCAAAAAGGC‑AACAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3133564/68‑1 (MQ=255)
gaagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3192376/67‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1918205/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1157046/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:463072/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2693911/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3273290/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2135086/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1850019/69‑1 (MQ=255)
gcCAAAAAGGCGACCAGCGGTGGGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3318990/69‑1 (MQ=255)
ccAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1087085/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:377234/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:621347/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:780850/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:898740/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3360069/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:950881/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2865399/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3138731/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1075419/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1304150/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1381379/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1419551/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1525201/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1555423/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2738739/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:274505/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2746573/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2942948/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2946167/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2974260/68‑1 (MQ=255)
cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3283693/68‑1 (MQ=255)
aaaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1650167/67‑1 (MQ=255)
aaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2850663/66‑1 (MQ=255)
aaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:802090/64‑1 (MQ=255)
ggCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1286539/62‑1 (MQ=255)
ggTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAc < 1:1713093/69‑1 (MQ=255)
ggTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAc < 1:877557/69‑1 (MQ=255)
|
GGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAC > minE/2464489‑2464618
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
