Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,676,470 |
T→A |
83.4% |
P73P (CCT→CCA) |
bioH → |
carboxylesterase of pimeloyl‑CoA synthesis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,676,470 | 0 | T | A | 83.4%
| 83.1
/ 9.4
| 42 | P73P (CCT→CCA) | bioH | carboxylesterase of pimeloyl‑CoA synthesis |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base A (15/20); total (17/25) |
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01 |
GGGATTTGGTGCGCTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGTT > minE/2676413‑2676529
|
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:2797456/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:944463/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:756307/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:646395/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:494817/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:380540/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:365773/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:218651/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:2038306/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:12236/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:1513076/1‑68 (MQ=255)
gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:206680/1‑68 (MQ=255)
aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:2092178/1‑69 (MQ=255)
aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:2949905/1‑69 (MQ=255)
aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:248100/1‑69 (MQ=255)
gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:3217080/69‑1 (MQ=255)
gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:808959/69‑1 (MQ=255)
gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:2145700/69‑1 (MQ=255)
gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:2362119/69‑1 (MQ=255)
gCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCtggt > 1:1237504/1‑68 (MQ=255)
gCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCtggt > 1:627401/1‑68 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGTAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:748157/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2200427/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:1162395/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:860025/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:840830/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2193695/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:700041/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:559603/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2432183/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:183190/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:322476/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2406677/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2879801/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:1045963/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2640189/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2614997/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2600790/69‑1 (MQ=255)
aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCCGGCTGGTGGCAAGCCAGATTGCGtt < 1:3068496/69‑1 (MQ=255)
cAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCATATTGCGtt < 1:1721778/68‑1 (MQ=255)
cAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:836879/68‑1 (MQ=255)
aGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAg < 1:669730/59‑1 (MQ=255)
|
GGGATTTGGTGCGCTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGTT > minE/2676413‑2676529
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
