Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,797,892 C→G 38.6% A426G (GCT→GGT)  dinF → DNA‑damage‑inducible SOS response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,797,8920CG38.6% 55.2 / 82.1 88A426G (GCT→GGT) dinFDNA‑damage‑inducible SOS response protein
Reads supporting (aligned to +/- strand):  ref base C (16/38);  new base G (10/24);  total (26/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

AGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTC  >  minE/2797825‑2797960
                                                                   |                                                                    
aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGc                                                                      <  1:963224/68‑1 (MQ=255)
aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGc                                                                      <  1:458323/68‑1 (MQ=255)
aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGc                                                                      <  1:837169/68‑1 (MQ=255)
aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGc                                                                      <  1:915107/68‑1 (MQ=255)
aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGc                                                                      <  1:1358204/68‑1 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgt                                                           <  1:509056/69‑2 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1917710/69‑1 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1850695/69‑1 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:168181/69‑1 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:2207858/69‑1 (MQ=255)
          tGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:3001348/69‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctggc                                                          >  1:2325066/1‑69 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctggc                                                          >  1:2622442/1‑69 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctggc                                                          >  1:2279599/1‑69 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctggc                                                          >  1:3431749/1‑69 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:877681/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:744173/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:2998737/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:654731/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1741625/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1185097/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1956121/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:1970192/68‑1 (MQ=255)
           ggCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:2941746/68‑1 (MQ=255)
            gccgccGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctgg                                                           <  1:2720876/67‑1 (MQ=255)
              cgccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGTCAtt                                                       <  1:2545229/69‑1 (MQ=255)
              cgccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                       <  1:2162442/69‑1 (MQ=255)
              cgccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                       <  1:1616501/69‑1 (MQ=255)
              cgccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                       <  1:2701696/69‑1 (MQ=255)
              cgccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                       <  1:1539774/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:71629/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:294026/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:2386896/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:3221660/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:3247489/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:612576/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:51548/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:2040665/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:2172883/69‑1 (MQ=255)
               gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCAGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTa                                                      <  1:1231328/69‑1 (MQ=255)
                             gcTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTgg                                         <  1:3240529/68‑1 (MQ=255)
                                gACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGtt                                     >  1:984234/1‑69 (MQ=255)
                                gACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGtt                                     >  1:3141442/1‑69 (MQ=255)
                                gACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGtt                                     >  1:1276736/1‑69 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:3334847/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:3401221/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:1314970/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:2658139/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:23604/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:2861480/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:811105/68‑1 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTtc                         >  1:3015923/1‑68 (MQ=255)
                                             cTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGGCTTTCTGGCGTTGCGCGGGCTTtc                         <  1:3155584/68‑1 (MQ=255)
                                                ccGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                       <  1:3129840/35‑1 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:1106989/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:3247867/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:2529834/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:672863/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:2078945/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:3357931/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:2733171/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:589346/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:2866591/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:366579/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:1507590/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:1175419/49‑2 (MQ=255)
                                                     gCTGGGGAATCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGtta                                    <  1:2968764/49‑2 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:2456207/69‑1 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:1248820/69‑1 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:7999/69‑1 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:329540/69‑1 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:2054693/69‑1 (MQ=255)
                                                       tGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTa              <  1:314127/69‑1 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTgg         >  1:1249042/1‑69 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTgg         >  1:2174713/1‑69 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTgg         >  1:1550837/1‑69 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTgg         >  1:1991644/1‑69 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTg          >  1:474547/1‑68 (MQ=255)
                                                            aaTCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCG‑GGGCTTTCTCTGGCGGCTATCTgg         >  1:3203710/1‑68 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTGTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:2918466/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTgg                                         >  1:2095960/1‑36 (MQ=37)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:103969/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:82021/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:3313232/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:3243179/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:2637548/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:2343921/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:1568149/1‑69 (MQ=255)
                                                             aTCATGGTTTGTGGCTGGCGTTAACCGTCTTTCTGGCGTTACGTGGGCTTTCTCTGGCGGCTATCTggc        >  1:1974469/1‑69 (MQ=255)
                                                               cATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTggcgg      >  1:1006000/1‑69 (MQ=255)
                                                                aTGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTggcggc     >  1:708691/1‑69 (MQ=255)
                                                                  gCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   <  1:996034/69‑1 (MQ=255)
                                                                  gCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGGTGCGCGGGCTTTCTCTGGCGGCTa              <  1:3379282/58‑1 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:233503/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:1440146/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:1263433/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:1051271/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:96650/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGt   >  1:327307/1‑68 (MQ=255)
                                                                   cTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTc  <  1:2327227/69‑1 (MQ=255)
                                                                   |                                                                    
AGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTC  >  minE/2797825‑2797960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A