Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,973,590 |
T→G |
54.4% |
intergenic (+6/‑15) |
radA → / → nadR |
predicted repair protein/bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,973,590 | 0 | T | G | 54.4%
| 14.3
/ 43.4
| 46 | intergenic (+6/‑15) | radA/nadR | predicted repair protein/bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (5/16); new base G (21/4); total (26/20) |
| Fisher's exact test for biased strand distribution p-value = 6.71e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
TGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGC > minE/2973536‑2973652
|
tGGCGTTAAAAAACTCTCCGACGCGCTTATCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:1020587/68‑1 (MQ=255)
tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:2453238/68‑1 (MQ=255)
tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:3183153/68‑1 (MQ=255)
tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:2208495/68‑1 (MQ=255)
tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:563167/68‑1 (MQ=255)
tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:1859884/68‑1 (MQ=255)
aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg < 1:2717479/53‑1 (MQ=255)
aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg < 1:2927027/53‑1 (MQ=255)
aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATgtcgt < 1:2936668/69‑1 (MQ=255)
ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTg < 1:102522/69‑1 (MQ=255)
ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTg < 1:431481/69‑1 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGTAGATATGTCGTCATTTGATTACCt > 1:2793820/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGAtt > 1:1933413/1‑64 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGAtt > 1:1323105/1‑64 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:490610/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:270583/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:565602/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2504698/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1123330/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1209676/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2450061/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2334491/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2313116/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1238523/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:3194645/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2074686/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1565204/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1908769/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:186220/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1619861/1‑68 (MQ=255)
cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:535226/1‑68 (MQ=255)
cgcgCTGAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:437638/1‑68 (MQ=255)
cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:568197/69‑1 (MQ=255)
cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2290776/69‑1 (MQ=255)
cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2601494/69‑1 (MQ=255)
cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:1086602/69‑1 (MQ=255)
gcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2164921/68‑1 (MQ=255)
ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTAcc > 1:1377022/1‑54 (MQ=255)
ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa > 1:2458703/1‑69 (MQ=255)
ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa > 1:1062437/1‑69 (MQ=255)
cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1002808/69‑1 (MQ=255)
cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1689937/69‑1 (MQ=255)
cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:610860/69‑1 (MQ=255)
taATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1967294/65‑1 (MQ=255)
aTGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc > 1:1929327/1‑69 (MQ=255)
aTGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc > 1:1767860/1‑69 (MQ=255)
|
TGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGC > minE/2973536‑2973652
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
