Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
619,771:1 |
+C |
100% |
coding (1078/1674 nt) |
rpsA → |
30S ribosomal subunit protein S1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 619,771 | 1 | . | C | 100.0%
| 187.2
/ NA
| 49 | T360P (ACC→CCC) | rpsA | 30S ribosomal subunit protein S1 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/49); total (0/49) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAA‑CCCACAACAAGGGCGACC > minE/619724‑619789
|
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:444595/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1003173/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1994917/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:2013475/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:254646/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:261131/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:270167/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:279287/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:28773/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:363197/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:416838/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:423702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1954214/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:524911/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:531991/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:566162/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:69204/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:702702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:728637/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:74689/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:802702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:81955/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:934997/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:95495/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1514688/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1062143/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1092082/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1162210/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1184989/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1258609/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:133595/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1370918/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1384716/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1386731/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1403077/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1919773/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1579235/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1625627/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1636895/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1724955/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1741487/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1754009/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1794229/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1799574/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1864611/67‑1 (MQ=255)
gggTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:327143/66‑1 (MQ=255)
gggTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1870288/66‑1 (MQ=255)
aaCCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:1132776/44‑1 (MQ=38)
gcagcaGTTCGCGGAAACCCCACAACAAGGGCGAcc < 1:851314/36‑1 (MQ=255)
|
TGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAA‑CCCACAACAAGGGCGACC > minE/619724‑619789
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
