Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,890,961 |
G→A |
100% |
P208S (CCG→TCG) |
gudP ← |
predicted D‑glucarate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,890,961 | 0 | G | A | 100.0%
| 107.3
/ NA
| 31 | P208S (CCG→TCG) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/0); total (31/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCT > minE/1890895‑1891003
|
gCTGATCCATATTGATCAGCGCTCCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACGCCCgg > 1:185327/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1952818/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:98740/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:978410/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:92552/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:921660/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:727900/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:658990/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:619029/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:565211/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:211466/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:2056068/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:2015622/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1028767/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1920164/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1488217/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1358461/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1152860/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg > 1:1430621/1‑67 (MQ=255)
ttATTTACCCCCTAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1129824/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc > 1:213701/1‑55 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc > 1:573309/1‑55 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:781056/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1148932/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1076512/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:654812/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:649467/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1200430/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1034413/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:983626/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:321293/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:261940/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1031258/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:2132532/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:2154570/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:13318/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:2069716/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1349906/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1972278/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1959235/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1415108/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1922141/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1921760/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1891213/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1442150/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1774260/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1594435/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1518851/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:1489162/1‑56 (MQ=255)
ttATTTACCCCCGAATGTTGATTTGGATCGTGGATGACTTTCAACCAGATAAAGCt > 1:1238926/1‑56 (MQ=255)
|
GCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCT > minE/1890895‑1891003
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
