Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,890,961 |
G→A |
69.4% |
P208S (CCG→TCG) |
gudP ← |
predicted D‑glucarate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,890,961 | 0 | G | A | 69.4%
| 65.2
/ 39.9
| 49 | P208S (CCG→TCG) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/10); new base A (18/16); total (23/26) |
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAC > minE/1890893‑1891020
|
ttGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGa < 1:3305279/70‑1 (MQ=255)
ttGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGa < 1:2310563/70‑1 (MQ=255)
tCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGAt < 1:3390391/70‑1 (MQ=255)
tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:766399/1‑71 (MQ=255)
tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:1252820/1‑71 (MQ=255)
tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:2031621/1‑71 (MQ=255)
tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:2060957/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc > 1:738350/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc > 1:4244216/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCt < 1:2809255/70‑1 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:1139546/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:4188039/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:3651435/1‑71 (MQ=255)
ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:190255/1‑71 (MQ=255)
aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:987301/71‑1 (MQ=255)
aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:2711960/71‑1 (MQ=255)
aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:2179619/71‑1 (MQ=255)
aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAAGGTTGATTTGGCTCGTg < 1:3732988/71‑1 (MQ=255)
gcgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:1898702/71‑1 (MQ=255)
gcgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:180146/71‑1 (MQ=255)
cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:167243/70‑1 (MQ=255)
caccCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTc < 1:416127/71‑1 (MQ=255)
accCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCa < 1:3782310/71‑1 (MQ=255)
cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTg < 1:3374103/59‑1 (MQ=255)
cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCaa > 1:4020611/1‑71 (MQ=255)
cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGtt < 1:3219993/59‑2 (MQ=255)
tACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:2783679/1‑71 (MQ=255)
tACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt < 1:469009/71‑1 (MQ=255)
aCTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACtt > 1:1868096/1‑54 (MQ=255)
ccAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc > 1:1210315/1‑66 (MQ=255)
cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatga > 1:1027094/1‑71 (MQ=255)
cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:3558120/70‑1 (MQ=255)
cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:1280107/70‑1 (MQ=255)
cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:3514757/70‑1 (MQ=255)
gCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACa < 1:3231462/71‑1 (MQ=255)
ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:3596497/1‑45 (MQ=255)
ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:370118/1‑45 (MQ=255)
ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:4206692/1‑45 (MQ=255)
tttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:1764310/40‑1 (MQ=255)
tttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:2976259/40‑1 (MQ=255)
tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATccc > 1:1777746/1‑70 (MQ=255)
tcttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc < 1:3256431/68‑1 (MQ=255)
tcttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc < 1:3079609/68‑1 (MQ=255)
cttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATccc > 1:3856114/1‑69 (MQ=255)
aTTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCa < 1:1298944/68‑1 (MQ=255)
aTTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAc > 1:3625218/1‑71 (MQ=255)
tttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCa < 1:3512657/67‑1 (MQ=255)
ttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACaa > 1:2822508/1‑60 (MQ=255)
ttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACaa > 1:1806485/1‑60 (MQ=255)
|
TTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAC > minE/1890893‑1891020
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
