Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,866,115:1 |
+C |
100% |
coding (68/198 nt) |
yjeT → |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,866,115 | 1 | . | C | 100.0%
| 24.2
/ NA
| 8 | Y23S (TAC→TCC) | yjeT | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/0); total (8/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AATTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑CCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGC > minE/2866051‑2866159
|
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1942205/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:938276/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:733538/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:502887/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:449694/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:424427/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:381458/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2024326/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1968055/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1945870/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1720929/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:16668/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1563699/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:150795/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1500425/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:142580/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1165511/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1403711/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:140152/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1365934/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:123350/67‑1 (MQ=255)
aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1275662/67‑1 (MQ=255)
aatTCGACAATCTCGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1224322/67‑1 (MQ=255)
atTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1999861/66‑1 (MQ=255)
atTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2008213/66‑1 (MQ=255)
tggcGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2037251/51‑1 (MQ=255)
ccTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1644756/41‑1 (MQ=255)
ttttGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1576152/36‑1 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATTATCTCTGCGATGACCAATTTGc > 1:1054104/1‑66 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCg > 1:218556/1‑53 (MQ=39)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATTACCAATTTg > 1:1159331/1‑65 (MQ=38)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:395074/1‑66 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:1299058/1‑66 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:330627/1‑66 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:651068/1‑66 (MQ=255)
aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:675354/1‑66 (MQ=255)
|
AATTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑CCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGC > minE/2866051‑2866159
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
