Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F64 I0 R1 19 139.0 6703000 96.6% 6475098 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,202 T→G 100% L99R (CTC→CGC)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,2020TG99.2% 453.5 / ‑3.8 128L99R (CTC→CGC) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (73/54);  total (74/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 128 total aligned reads displayed.

GGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGC  >  NC_000913/756106‑756298
                                                                                                |                                                                                                
  cATATGTACGACAGCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGGAAAGCCGGGCCGGAAGCGATTCTGGAACGCGAac                                                                                             <  2:253047/100‑1 (MQ=255)
  cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAac                                                                                             <  2:829623/100‑1 (MQ=255)
   atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAaca                                                                                            >  2:1446895/1‑100 (MQ=255)
   atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAaca                                                                                            <  1:2307236/100‑1 (MQ=255)
   atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAaca                                                                                            >  1:2119879/1‑100 (MQ=255)
    tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAacac                                                                                           >  1:2512249/1‑100 (MQ=255)
    tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAacac                                                                                           >  2:84915/1‑100 (MQ=255)
     atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAAcaca                                                                                          >  1:2246215/1‑100 (MQ=255)
      tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAAcaca                                                                                          >  2:610811/1‑99 (MQ=255)
      tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACAt                                                                                         >  2:3322298/1‑100 (MQ=255)
         ncGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGCTTGAATATATGTGTAAAAAAGGGCCGGAAGCGATTCTGGCACGCGAACACAttcg                                                                                      >  1:2975373/2‑97 (MQ=255)
          cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGc                                                                                     >  2:355407/1‑100 (MQ=255)
          cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGc                                                                                     >  1:1474405/1‑100 (MQ=255)
           gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc                                                                                    <  1:1074377/100‑1 (MQ=255)
           gACACCGGGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc                                                                                    <  1:1291545/100‑1 (MQ=255)
             ctccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTg                                                                                  <  1:98250/98‑1 (MQ=255)
             cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTg                                                                                  >  2:951024/1‑100 (MQ=255)
             cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTg                                                                                  >  2:2664823/1‑100 (MQ=255)
              acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGc                                                                                 <  2:1504554/100‑1 (MQ=255)
               ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTgag                                                                                >  2:98251/1‑98 (MQ=255)
               ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGcc                                                                                >  1:1302021/1‑100 (MQ=255)
               ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGcc                                                                                >  1:1189332/1‑100 (MQ=255)
                cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGGTTGAATATATGTGTAACACCGGGCCGGAAGCGTTTCTGGAACGCGAACACATGGGCCTGCCg                                                                               >  2:1477986/1‑100 (MQ=255)
                cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCg                                                                               >  1:194605/1‑100 (MQ=255)
                cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCg                                                                               <  1:1175669/100‑1 (MQ=255)
                 gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGt                                                                              >  1:41306/1‑100 (MQ=255)
                   gAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtc                                                                            >  1:430501/1‑100 (MQ=255)
                     aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtctc                                                                          <  1:1980851/100‑1 (MQ=255)
                      aGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtctcn                                                                         <  2:1735661/100‑2 (MQ=255)
                        ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTcgcg                                                                       <  1:1305669/100‑1 (MQ=255)
                        ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCATGACGTTATcga                                                                        >  1:3110529/1‑98 (MQ=255)
                         gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGt                                                                      >  2:2637062/1‑100 (MQ=255)
                          tCGAACTATATCGGTGACCAGGACGTGATTGGATATATGTGTAAAACCGGGCCGGAAGCGATGCTGGAACGCGAACACATGGGCCTGTCGTTCTcgcg                                                                       <  1:2904681/98‑1 (MQ=255)
                            ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGtctc                                                                   <  1:994085/100‑1 (MQ=255)
                               cTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                                                <  1:1820205/100‑1 (MQ=255)
                                tataTCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatg                                                               <  2:195165/100‑1 (MQ=255)
                                 atatCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatga                                                              >  2:2366746/1‑100 (MQ=255)
                                   atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                                            <  1:3252304/100‑1 (MQ=255)
                                   atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                                            <  2:3205050/100‑1 (MQ=255)
                                       gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                                        <  2:1262711/100‑1 (MQ=255)
                                       gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                                        <  2:210572/100‑1 (MQ=255)
                                         gACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                      <  2:262310/100‑1 (MQ=255)
                                             aGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                                                  >  1:381684/1‑100 (MQ=255)
                                              ggACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                                                 >  1:1975965/1‑100 (MQ=255)
                                              ggACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAAAACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                                                 >  1:1814816/1‑100 (MQ=255)
                                               gACGGGATTGAATATATGTGTAAAACCGGGCCGGACGCTATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                                                <  1:664422/100‑1 (MQ=255)
                                                aCGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCa                                               >  2:2686446/1‑100 (MQ=255)
                                                 ggcgATTGAATATATGTGTAAAACCGGGCCTGAGGGGATTCTGGATCGCGAACACATGGGCCTGCCGTTCTTGCGTCTCGATGATGGTCGtatcta                                                  <  1:3539/95‑1 (MQ=255)
                                                 cgcgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                                              >  2:2579786/1‑100 (MQ=255)
                                                 cgcgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                                              >  1:1585989/1‑100 (MQ=255)
                                                  gcgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                             >  2:1017874/1‑100 (MQ=255)
                                                   cgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                            >  2:3236160/1‑100 (MQ=255)
                                                   cgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                            >  2:980000/1‑100 (MQ=255)
                                                       tGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCg                                        <  1:1912100/100‑1 (MQ=255)
                                                           tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg                                    >  1:1170101/1‑100 (MQ=255)
                                                           tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg                                    >  1:2329825/1‑100 (MQ=255)
                                                           tatatCTGTAAAACTGTGCCGGAAGCGATTCTGTGACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt                                      <  1:887258/98‑1 (MQ=255)
                                                             tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                  <  2:133025/100‑1 (MQ=255)
                                                             tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                  <  2:689388/100‑1 (MQ=255)
                                                             tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTCGGc                                  >  1:3066259/1‑100 (MQ=255)
                                                              atGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg                                 <  1:972365/100‑1 (MQ=255)
                                                              atGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg                                 <  2:1602003/100‑1 (MQ=255)
                                                               tgtgTAAAACCGGGCCGGAAGCGAATCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCATCTCAATGATGGTCGTATCTATCAACGTCCGTTTAGCg                                 >  1:1269362/1‑99 (MQ=255)
                                                                  gtAAAACCGGGCCGGAATCGATTCTGGAACGCGGACACAGGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                              <  1:2942960/99‑1 (MQ=255)
                                                                  gtAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa                             >  1:1357572/1‑100 (MQ=255)
                                                                  gtAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa                             >  1:1367332/1‑100 (MQ=255)
                                                                   tAAAACCGGGCCGGAAGCGATTCTGCAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg                            >  2:1293356/1‑100 (MQ=255)
                                                                    aaaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt                           >  2:492152/1‑100 (MQ=255)
                                                                    aaaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt                           >  2:1800578/1‑100 (MQ=255)
                                                                     aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                          >  1:3058629/1‑100 (MQ=255)
                                                                     aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                          >  1:1719589/1‑100 (MQ=255)
                                                                      aaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCg                         <  2:1302025/100‑1 (MQ=255)
                                                                        ccgggccgggAGCCATTCTGGTACGCGAACACTTGATCCTGCCATTCAACCGTCGCGATGATGGTCGTCTCTATCAACGTCCGCTTGGCAGTTAGt                           >  1:2743308/1‑96 (MQ=255)
                                                                        ccGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa                       >  2:2320078/1‑100 (MQ=255)
                                                                           ggCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                    >  1:2369712/1‑100 (MQ=255)
                                                                             ccGGAAGGGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACt                  <  1:432534/100‑1 (MQ=255)
                                                                             ccGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAAcn                  <  2:1365339/100‑2 (MQ=255)
                                                                             ccGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACt                  <  2:2192021/100‑1 (MQ=255)
                                                                               ggAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc                >  1:1587858/1‑100 (MQ=255)
                                                                               ggAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc                >  2:3103094/1‑100 (MQ=255)
                                                                                gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg               <  1:812043/100‑1 (MQ=255)
                                                                                gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg               <  2:2526948/100‑1 (MQ=255)
                                                                                 aaGCGATCCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcgg              <  2:2351393/100‑1 (MQ=255)
                                                                                  aGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc             >  1:3074522/1‑100 (MQ=255)
                                                                                  aGCGATTCGGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc             <  1:2592030/100‑1 (MQ=255)
                                                                                   gCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg            >  2:2502934/1‑100 (MQ=255)
                                                                                    cGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcgg           >  2:2241909/1‑100 (MQ=255)
                                                                                     gATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc          >  1:3192786/1‑100 (MQ=255)
                                                                                     gATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc          >  1:1497561/1‑100 (MQ=255)
                                                                                      aTTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcg         >  1:2118926/1‑100 (MQ=255)
                                                                                       ttCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTTTCAACGTCCGCTTGGCGGTCAGTCGAAATACTTCggcggcta        <  2:2258509/100‑3 (MQ=255)
                                                                                       ttCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcgn        <  2:869194/100‑2 (MQ=255)
                                                                                        tCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAg       <  2:436313/100‑1 (MQ=255)
                                                                                         cTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc      <  2:402236/100‑1 (MQ=255)
                                                                                         cTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc      >  1:210083/1‑100 (MQ=255)
                                                                                         cTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc      >  1:2216943/1‑100 (MQ=255)
                                                                                           ggAACGCGAACATATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCa     <  1:1396758/99‑1 (MQ=255)
                                                                                           ggAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAg    >  2:1615097/1‑100 (MQ=255)
                                                                                           ggAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAg    >  2:241053/1‑100 (MQ=255)
                                                                                             aaCGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAggc  >  2:791147/1‑100 (MQ=255)
                                                                                                |                                                                                                
GGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGC  >  NC_000913/756106‑756298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A