Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F49 I0 R1 48 144.3 7113499 94.4% 6715143 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,929 T→A 28.5% D341E (GAT→GAA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,9290TA28.5% 176.7 / 92.4 116D341E (GAT→GAA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (28/55);  new base A (15/18);  total (43/73)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

Only 100 of 116 total aligned reads displayed.

CCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGA  >  NC_000913/756831‑757024
                                                                                                  |                                                                                               
ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                                                                >  2:780107/1‑100 (MQ=255)
 cacaCGCGAAACTGATACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc                                                                                               <  2:3496911/100‑1 (MQ=255)
 cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAAcc                                                                                               <  2:3078377/100‑1 (MQ=255)
  acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCg                                                                                              <  1:2036659/100‑1 (MQ=255)
    acGCGGAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGt                                                                                            <  1:1196930/100‑1 (MQ=255)
    acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                                                                            <  2:75170/100‑1 (MQ=255)
     cgcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTg                                                                                           >  2:1287048/1‑100 (MQ=255)
     cgcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTg                                                                                           <  2:3514459/100‑1 (MQ=255)
       cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCTGGTGaa                                                                                         >  2:369744/1‑100 (MQ=255)
       cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                                                                         <  1:1126580/100‑1 (MQ=255)
          aactgaacctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCTGGTGAAag                                                                                       <  1:2620437/99‑1 (MQ=255)
          aactgaaactCGATCACCTGGGTAACGAAGTTCTCGAATCCCGTCTGCCGGGCATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCCGTGAAcga                                                                                      >  1:244641/1‑100 (MQ=255)
            ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc                                                                                    >  1:1261996/1‑100 (MQ=255)
             tgaaactCGATCACCTGGGTGAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                   <  1:276341/100‑1 (MQ=255)
             tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                   <  2:1678109/100‑1 (MQ=255)
             tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGc                                                                                    <  1:2107667/99‑1 (MQ=255)
              gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                  <  2:1136059/100‑1 (MQ=255)
              gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCg                                                                                  >  1:3550228/1‑100 (MQ=255)
                aactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGAt                                                                                <  2:2311789/100‑1 (MQ=255)
                  ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGATTTCCCGTACCTTCGCTCACGTCGATTCGGTGAAAGAGCCGATTc                                                                              >  1:2720801/1‑100 (MQ=255)
                  ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGCGTATCCTGGAGCTTTCCCGTCCCTTCGCTCACGTCGATCAGGTGAAAGAGCCGATTc                                                                              >  1:1356777/1‑100 (MQ=255)
                   tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTcc                                                                             <  2:618457/100‑1 (MQ=255)
                     gATCACCTGGGTGAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTGCGCTCACGTCGATCCGAAGAAAGAGCCGATTCCgg                                                                           >  1:1080510/1‑100 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCGTGGAGATTTCCCGTACCTTCGCTCACGTCGATCCGGCGAAAGAGCCGATTCCgg                                                                           >  1:2472195/1‑100 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCTGGTGAAAGAGCCGATTCCgg                                                                           <  1:780103/100‑1 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCgg                                                                           <  1:2726065/100‑1 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCgg                                                                           <  1:3466484/100‑1 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCgg                                                                           <  2:2342272/100‑1 (MQ=255)
                     gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAGCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                                                            >  1:2672898/1‑99 (MQ=255)
                      aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                                                          <  2:2741851/100‑1 (MQ=255)
                      aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                                                          <  1:2986067/100‑1 (MQ=255)
                        cACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                                                                        >  2:1302588/1‑100 (MQ=255)
                          ccTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATc                                                                      >  2:85376/1‑100 (MQ=255)
                          ccTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATc                                                                      >  2:549128/1‑100 (MQ=255)
                           cTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc                                                                     <  2:2255101/100‑1 (MQ=255)
                             gggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCa                                                                   >  2:1261464/1‑100 (MQ=255)
                               gTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAc                                                                 <  2:3224765/100‑1 (MQ=255)
                               gTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAc                                                                 <  1:956319/100‑1 (MQ=255)
                                tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCTGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                <  1:960527/100‑1 (MQ=255)
                                tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                >  2:3190792/1‑100 (MQ=255)
                                tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                <  1:3403622/100‑1 (MQ=255)
                                tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                <  1:2722331/100‑1 (MQ=255)
                                tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                <  2:160417/100‑1 (MQ=255)
                                  aagaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg                                                              >  2:2792448/1‑100 (MQ=255)
                                     aagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCa                                                           <  2:1760135/100‑1 (MQ=255)
                                      agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                          <  2:3531819/100‑1 (MQ=255)
                                       gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATACGGTGCAAGAGCCAATTCCGGGTATCCCAACCTGTCAc                                                          >  2:1071653/1‑99 (MQ=255)
                                       gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTCTCCCAACCTGTCACt                                                         >  2:3504682/1‑100 (MQ=255)
                                       gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                         >  2:2287282/1‑100 (MQ=255)
                                       gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                         >  2:1208517/1‑100 (MQ=255)
                                       gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                         >  2:3447910/1‑100 (MQ=255)
                                        ttCTCGAATCCCGTCTGCCGGGTAGCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTa                                                        <  1:2264856/100‑1 (MQ=255)
                                         tctcGAATCCCGTCTGCCGGGTATCCCGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTAc                                                       >  1:2605528/1‑100 (MQ=255)
                                           tcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACat                                                     <  2:2631594/100‑1 (MQ=255)
                                             gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCGGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGAGTCCGGTTATCCCAACCTGTCACTACatg                                                    >  1:2129201/1‑99 (MQ=255)
                                             gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga                                                   >  1:1816276/1‑100 (MQ=255)
                                                tCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATgg                                                >  2:3346931/1‑100 (MQ=255)
                                                  ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCTGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc                                              <  2:2355636/100‑1 (MQ=255)
                                                   cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                             <  2:307669/100‑1 (MQ=255)
                                                    gTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg                                            <  1:1480603/100‑1 (MQ=255)
                                                      cTGCCGGGGATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGt                                           <  2:1928619/99‑1 (MQ=255)
                                                        gCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCAGGTTATCCCAACCTGTCACTACATGATGGGCGGTAt                                         >  2:492666/1‑99 (MQ=255)
                                                         ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                       <  1:2248035/100‑1 (MQ=255)
                                                          cGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                      <  2:1712961/100‑1 (MQ=255)
                                                           gggTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                     <  1:2792432/100‑1 (MQ=255)
                                                           gggTAGCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                     <  2:1261745/100‑1 (MQ=255)
                                                            ggTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                    <  2:1548628/100‑1 (MQ=255)
                                                             gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                   >  1:2389932/1‑100 (MQ=255)
                                                             gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                   <  2:3414879/100‑1 (MQ=255)
                                                             gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                   <  2:401747/100‑1 (MQ=255)
                                                              tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCTGTATTCCGAcc                                  <  1:1856946/100‑1 (MQ=255)
                                                               ctCCTGGGGCTTTCCCGTGTCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCa                                 <  1:3006469/99‑1 (MQ=255)
                                                                  cTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                              >  1:321958/1‑100 (MQ=255)
                                                                  cTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                              <  2:323624/100‑1 (MQ=255)
                                                                   tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGt                             <  2:2072259/100‑1 (MQ=255)
                                                                   tGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGt                             >  1:1342568/1‑100 (MQ=255)
                                                                     gAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTa                           <  1:1612500/100‑1 (MQ=255)
                                                                      aGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                          <  1:1827238/100‑1 (MQ=255)
                                                                       gCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                         >  2:185553/1‑100 (MQ=255)
                                                                         tttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCgg                       <  1:545972/100‑1 (MQ=255)
                                                                         tttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCgg                       <  1:3382224/100‑1 (MQ=255)
                                                                           tCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTc                     >  1:3187258/1‑100 (MQ=255)
                                                                              cGTACCTTCGCTCACGTCGATCTGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                  <  2:891388/100‑1 (MQ=255)
                                                                              cGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                  >  2:656875/1‑100 (MQ=255)
                                                                               gTACCTTCGCTCACGTCGATCTGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGc                 >  2:2718137/1‑100 (MQ=255)
                                                                               gTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGc                 <  1:3184031/100‑1 (MQ=255)
                                                                                tACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCa                <  2:3114816/100‑1 (MQ=255)
                                                                                  ccTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgtgcct              <  2:1464750/100‑6 (MQ=255)
                                                                                   cTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactg             <  2:2796599/100‑1 (MQ=255)
                                                                                   cTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactg             <  2:2105764/100‑1 (MQ=255)
                                                                                     tCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac           <  2:840676/100‑1 (MQ=255)
                                                                                        cTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt        >  2:2228846/1‑100 (MQ=255)
                                                                                         tCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg       <  2:1816285/100‑1 (MQ=255)
                                                                                          cACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa      >  2:1229267/1‑100 (MQ=255)
                                                                                           aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGaa     <  1:2105533/100‑1 (MQ=255)
                                                                                           aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGaa     <  2:2623724/100‑1 (MQ=255)
                                                                                            cGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAAt    <  1:1776450/100‑1 (MQ=255)
                                                                                              tCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga  <  2:1117926/100‑1 (MQ=255)
                                                                                              tCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga  <  1:1583233/100‑1 (MQ=255)
                                                                                              tCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga  <  2:880765/100‑1 (MQ=255)
                                                                                                  |                                                                                               
CCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGA  >  NC_000913/756831‑757024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A