Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F64 I1 R1
|
17 |
68.2 |
3417408 |
93.4% |
3191859 |
100.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
756,202 |
T→G |
L99R (CTC→CGC) |
sdhA → |
succinate dehydrogenase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 756,202 | 0 | T | G | 98.2%
| 207.3
/ ‑4.4
| 56 | L99R (CTC→CGC) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (22/33); total (22/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
TGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC > NC_000913/756105‑756294
|
gatcttatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAcgcg < 2:1495943/95‑1 (MQ=255)
gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCCAGGACGCGATTGAATATATGTGTCAAACCGGGCCGGAAGCGATTCTGGAACGCGa > 2:1000727/1‑99 (MQ=255)
cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAac < 2:418086/100‑1 (MQ=255)
cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAac < 1:418086/100‑1 (MQ=255)
gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:2184633/100‑1 (MQ=255)
gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 1:316115/100‑1 (MQ=255)
gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:316115/100‑1 (MQ=255)
gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:891356/100‑1 (MQ=255)
aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATggg < 2:2210815/100‑1 (MQ=255)
gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc > 2:402670/1‑100 (MQ=255)
gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc > 1:402670/1‑100 (MQ=255)
cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTg > 2:2267104/1‑100 (MQ=255)
acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGc < 1:573258/100‑1 (MQ=255)
acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGc < 2:573258/100‑1 (MQ=255)
aaaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtct > 2:282188/1‑100 (MQ=255)
aaaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtct > 1:282188/1‑100 (MQ=255)
ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTcgcg < 2:1449600/100‑1 (MQ=255)
gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGtctc < 2:851182/99‑1 (MQ=255)
gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCg > 1:589949/1‑100 (MQ=255)
gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCg > 2:589949/1‑100 (MQ=255)
aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCga < 2:618562/100‑1 (MQ=255)
aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCga < 1:618562/100‑1 (MQ=255)
tCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg > 2:1876405/1‑100 (MQ=255)
gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:716617/100‑1 (MQ=255)
gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 1:716617/100‑1 (MQ=255)
gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:1759590/100‑1 (MQ=255)
ccAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGGTGGTCGtatc > 2:672949/1‑100 (MQ=255)
ccAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGGTGGTCGtatc > 1:672949/1‑100 (MQ=255)
cAGGACGCGATTGAATATATGTGTAAATCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct < 1:626981/100‑1 (MQ=255)
cAGGACGCGATTGAATATATGTGTAAATCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct < 2:626981/100‑1 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc > 2:1711737/1‑100 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 2:371182/100‑1 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 1:371182/100‑1 (MQ=255)
gAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt > 2:1936368/1‑100 (MQ=255)
aaTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt > 2:700271/1‑100 (MQ=255)
aaTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt > 1:700271/1‑100 (MQ=255)
atatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt < 2:164335/100‑1 (MQ=255)
atatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt < 1:164335/100‑1 (MQ=255)
tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg > 2:722936/1‑100 (MQ=255)
tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg > 1:722936/1‑100 (MQ=255)
tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg > 2:2096337/1‑100 (MQ=255)
aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 2:344570/100‑1 (MQ=255)
aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 2:15667/100‑1 (MQ=255)
aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 1:344570/100‑1 (MQ=255)
aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 1:15667/100‑1 (MQ=255)
ccGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa < 2:1864476/100‑1 (MQ=255)
ccGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACt < 2:931741/100‑1 (MQ=255)
gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 2:316234/1‑100 (MQ=255)
gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 2:1748832/1‑100 (MQ=255)
gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg < 2:1640721/100‑1 (MQ=255)
gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 1:316234/1‑100 (MQ=255)
aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcgg < 2:2043160/100‑1 (MQ=255)
aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTcg < 2:284139/99‑1 (MQ=255)
aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTcg < 1:284139/99‑1 (MQ=255)
aGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc < 2:1200161/100‑1 (MQ=255)
gATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc > 2:1429179/1‑100 (MQ=255)
ttGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc < 2:2485525/99‑1 (MQ=255)
|
TGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC > NC_000913/756105‑756294
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
