Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F52 I0 R1 129 152.3 7640533 93.0% 7105695 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,150 C→T 17.5% intergenic (‑201/+28) ibsC ← / ← serA toxic membrane protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1500CT17.5% 434.5 / 67.1 181intergenic (‑201/+28)ibsC/serAtoxic membrane protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (70/79);  new base T (14/18);  total (84/97)
Fisher's exact test for biased strand distribution p-value = 8.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 189 total aligned reads displayed.

ATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTC  >  NC_000913/3057051‑3057249
                                                                                                    |                                                                                                   
aTCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGAcc                                                                                                     <  1:3053822/100‑1 (MQ=255)
  cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTg                                                                                                   >  2:1299934/1‑100 (MQ=255)
   tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGc                                                                                                  >  1:495755/1‑100 (MQ=255)
    gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                                 <  2:3184292/100‑1 (MQ=255)
      cTTTGATTTATTTTCTGAAGCAGACACGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                               <  1:3591435/100‑1 (MQ=255)
          gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGGGCAACGCGGCAACGGTCTGGAGAAGGGAT‑AAATCAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                           <  1:650437/100‑1 (MQ=255)
          gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                           <  2:3686323/100‑1 (MQ=255)
          gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                           >  2:3449122/1‑100 (MQ=255)
          gATTCATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                           <  2:2454340/100‑1 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                                          <  1:1136624/100‑4 (MQ=38)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                          >  1:2656940/1‑100 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                          >  1:1958492/1‑100 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                          >  1:1990632/1‑100 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                          >  2:500907/1‑100 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGCCAGTGACCTGCCCGTTGa                                                                                           >  1:2555775/1‑99 (MQ=255)
           atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACCGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                          >  1:460521/1‑100 (MQ=255)
            tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGtttttt                                                                                         <  2:2338706/100‑5 (MQ=38)
            tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGtttttt                                                                                         <  2:207077/100‑5 (MQ=38)
            tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                                         <  1:2322926/100‑1 (MQ=255)
             ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGttttttt                                                                                        <  1:3357659/100‑6 (MQ=37)
             ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGttttttt                                                                                        <  2:3033289/100‑6 (MQ=37)
             ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                        <  2:3743054/100‑1 (MQ=255)
             ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                        >  2:3327782/1‑100 (MQ=255)
             ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                        <  1:2497608/100‑1 (MQ=255)
              tatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtttt                                                                                       >  2:979734/1‑100 (MQ=255)
               atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                      >  1:3653643/1‑100 (MQ=255)
               atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                      <  2:1037093/100‑1 (MQ=255)
               atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                      >  2:250991/1‑100 (MQ=255)
                 tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatcc                                                                                    <  2:2931118/100‑10 (MQ=25)
                 tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatcc                                                                                    <  2:2810305/100‑10 (MQ=25)
                 tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                    <  2:1349422/100‑1 (MQ=255)
                 tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                    <  1:2773475/100‑1 (MQ=255)
                 tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTAGAAAAAGAAA‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                    <  1:3359/100‑1 (MQ=255)
                   tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagc                                                                                   <  1:630289/99‑2 (MQ=255)
                   tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                                  >  2:2762288/1‑100 (MQ=255)
                   tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                                  >  2:729750/1‑100 (MQ=255)
                   tCTGGAGCAGACTCGAAAAAAAGAAAACGCAACGCAGCAACGGTGTGGAGAAGTGAT‑AAAAAAACGAGCAGGTCACTGACTTGCCCGtttttttat                                                                                      <  1:3730505/96‑8 (MQ=16)
                   tCTGGAGCAGACCCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAAAAAAAAA‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCaga                                                                                   <  1:1243211/99‑1 (MQ=255)
                      ggAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                               >  2:2054441/1‑100 (MQ=255)
                       gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                              >  1:1083150/1‑100 (MQ=255)
                       gAGCAAACTGGCAAAGGAGAATGCGAAACGCGGCAACGGTGTGGAGATGGGAT‑AATTTATCGGGCAGTTGACTGACTTGCCCGtttttttatc                                                                                     <  1:3696938/93‑9 (MQ=12)
                         gCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                            <  1:538782/100‑1 (MQ=255)
                            gACTCGCGAAGTAGAATGCGCAACGCGGTAACGGTGGGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                            <  2:3806029/97‑1 (MQ=255)
                              cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                       <  2:3525285/100‑1 (MQ=255)
                                 tcAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGCCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                                    <  1:2107883/99‑1 (MQ=255)
                                 gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                                    <  2:671419/100‑1 (MQ=255)
                                 gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                                    >  2:413588/1‑100 (MQ=255)
                                  cAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTaa                                                                   >  2:2107893/1‑99 (MQ=255)
                                   aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                   >  2:1106125/1‑100 (MQ=255)
                                   aaaGTAGAATGAGCAACGCGGCAACGGTGTGGAAAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                  <  1:980679/100‑1 (MQ=255)
                                     aGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                                <  1:2166190/100‑1 (MQ=255)
                                      gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                               >  2:2991454/1‑100 (MQ=255)
                                      gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                               <  2:1192306/100‑1 (MQ=255)
                                      gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                               <  2:473668/100‑1 (MQ=255)
                                      gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                                >  1:3144679/1‑99 (MQ=255)
                                      gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTACTAcagca                                                               <  2:1996837/100‑1 (MQ=255)
                                        aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagcag                                                              >  2:2203589/1‑99 (MQ=255)
                                        aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGa                                                             >  1:637316/1‑100 (MQ=255)
                                            tGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                         >  2:1768442/1‑100 (MQ=255)
                                            tGCGCAACGCGGCAACCGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                         <  2:460431/100‑1 (MQ=255)
                                              cgcAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                                       <  2:1397438/100‑1 (MQ=255)
                                                     cgGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTCACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                                <  1:2447939/100‑1 (MQ=255)
                                                        cAACGGTGTGGAGAAGGGATAAAAAAAACGGGAAATCCGGTGCCTTGCCGGTTGATTTCCGGGGAAGGGGAATAGTTACAGCAAACggcgcggattg                                                 >  2:1576339/1‑88 (MQ=255)
                                                        cAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                             >  2:2410089/1‑100 (MQ=255)
                                                         aaCGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                            <  1:511548/100‑1 (MQ=255)
                                                                  nagaAGGGAT‑AAAAAAACGGGCTAGTCAGTGCCCTGCCCGTTGCTTTTCAGAGNAAGGGAACTAGTACGGCAGACGGGCGCGCATGGTccc                                            >  1:1579576/2‑91 (MQ=255)
                                                                  gagaAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                   <  2:1374187/100‑1 (MQ=255)
                                                                  gagaAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                   <  2:363768/100‑1 (MQ=255)
                                                                   agaAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGTGCGAATGGTACCCGGAATAGCt                                  >  1:3460803/1‑100 (MQ=255)
                                                                   agaAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCTCGAATGGTACCCGGAATAGCt                                  >  1:2056021/1‑100 (MQ=255)
                                                                     aaGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCttt                                <  1:39903/100‑1 (MQ=255)
                                                                     aaGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCttt                                <  1:1846009/100‑1 (MQ=255)
                                                                      aGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTc                               <  1:1331725/100‑1 (MQ=255)
                                                                        ggAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                             <  2:1990642/100‑1 (MQ=255)
                                                                        ggAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                             >  1:685626/1‑100 (MQ=255)
                                                                         gAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                            >  2:1788390/1‑100 (MQ=255)
                                                                          aT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg                           <  1:789719/100‑1 (MQ=255)
                                                                           t‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg                           >  1:793655/1‑99 (MQ=255)
                                                                           t‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                          <  2:3151194/100‑1 (MQ=255)
                                                                             aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                            <  1:1087057/97‑1 (MQ=255)
                                                                             aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                         <  2:1958502/100‑1 (MQ=255)
                                                                             aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                         >  2:1631337/1‑100 (MQ=255)
                                                                              aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                        <  2:571197/100‑1 (MQ=255)
                                                                              aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                        <  2:1069670/100‑1 (MQ=255)
                                                                                tcaaCGGGCAGGTAAATGACTTGCCCGTTGATTTTAAGAGAAGAGGAATTAATACAGCAGACGATCTCGAATGGTACTCGGAATAGCTTTAAtttc                          >  1:2233676/3‑94 (MQ=255)
                                                                                ncaaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                      >  2:1890160/3‑100 (MQ=38)
                                                                                aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                      <  2:2718148/100‑1 (MQ=255)
                                                                                 caaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                     >  1:1462500/2‑100 (MQ=38)
                                                                                   aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTCCAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                   >  1:3282607/1‑100 (MQ=38)
                                                                                   aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                   >  2:2872442/1‑100 (MQ=39)
                                                                                   aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAACCGGGATAGCGTTCATTGCCTGAAgc                   >  2:720988/1‑100 (MQ=38)
                                                                                   aCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                   <  1:2985608/100‑1 (MQ=255)
                                                                                    cGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                   >  1:1661945/1‑99 (MQ=255)
                                                                                      ggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                >  1:1887357/1‑100 (MQ=255)
                                                                                             cAGTGACCTGCCCGTTGATTTTCATAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCgg         <  2:3802794/100‑1 (MQ=255)
                                                                                              aGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGc        <  2:2625149/100‑1 (MQ=255)
                                                                                               gTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCa       <  1:83019/100‑1 (MQ=255)
                                                                                                 gACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc     <  1:729742/100‑1 (MQ=255)
                                                                                                 gACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc     <  1:2891608/100‑1 (MQ=255)
                                                                                                   ccTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGt   <  1:3803898/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
ATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTC  >  NC_000913/3057051‑3057249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A