Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F48 I1 R1
|
17 |
101.2 |
5204606 |
90.8% |
4725782 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
843,749 |
C→A |
D578Y (GAT→TAT) |
ybiO ← |
mechanosensitive channel protein, intermediate conductance |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 843,749 | 0 | C | A | 97.7%
| 295.2
/ ‑2.2
| 85 | D578Y (GAT→TAT) | ybiO | mechanosensitive channel protein, intermediate conductance |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (42/41); total (43/42) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
GAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAAT > NC_000913/843653‑843843
|
gAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCGAATATcc < 2:1365483/100‑1 (MQ=255)
atgatgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAg < 1:2574215/100‑1 (MQ=255)
atgatgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAg > 1:2207941/1‑100 (MQ=255)
gatgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAgtg > 2:1013523/1‑100 (MQ=255)
tgATACGCCCCGGTATCCTGTCGTACGCCCACGCAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATAACCAGTGTTCa > 1:1706304/1‑100 (MQ=255)
gATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCAt > 1:193661/1‑100 (MQ=255)
gATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCAt < 1:1946836/100‑1 (MQ=255)
gATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCAt > 1:2234569/1‑100 (MQ=255)
tACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGc > 2:124006/1‑100 (MQ=255)
gCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGt > 2:1130341/1‑100 (MQ=255)
ccccGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGtt > 1:845126/1‑100 (MQ=255)
ccGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGtttt < 2:1096500/100‑1 (MQ=255)
cGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTc < 2:250576/100‑1 (MQ=255)
ggTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCa > 2:2536044/1‑100 (MQ=255)
aTCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAAc < 2:834314/100‑1 (MQ=255)
aTCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAAc < 1:1179562/100‑1 (MQ=255)
tCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACt < 2:1293381/100‑1 (MQ=255)
ccTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTg < 2:2501348/100‑1 (MQ=255)
tGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaa > 2:631691/1‑100 (MQ=255)
gTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaat > 2:2129091/1‑100 (MQ=255)
tCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaata < 2:1858640/100‑1 (MQ=255)
tCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaata < 1:631690/100‑1 (MQ=255)
tCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaata < 1:1130338/100‑1 (MQ=255)
aCGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaataaata > 1:993685/1‑100 (MQ=255)
cGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAc > 1:1849104/1‑100 (MQ=255)
gCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAcc < 1:2005308/100‑1 (MQ=255)
cccACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCCAACTGAATAAATAccc > 1:1522248/1‑100 (MQ=255)
ccACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAcccc < 1:2434983/100‑1 (MQ=255)
ccgatctgactCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCgg < 1:1258345/90‑1 (MQ=255)
aCGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCgg < 2:2220472/100‑1 (MQ=255)
cgatctgAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGt < 2:2311424/94‑1 (MQ=255)
cGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGt < 1:226670/100‑1 (MQ=255)
cGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGt > 1:1861504/1‑100 (MQ=255)
ggAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAACTATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTg < 2:2038433/100‑1 (MQ=255)
ggAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTg < 2:1821025/100‑1 (MQ=255)
gCGAATCGACATCCGTTCCACTGTGCCGGTTAACGGGCCGATAGTCTCCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGat < 1:1753456/99‑1 (MQ=255)
cGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataa > 1:337746/1‑100 (MQ=255)
cGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataa > 1:680070/1‑100 (MQ=255)
gAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGtagatcg > 1:2311407/1‑94 (MQ=255)
gAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGGTCATGCCGATTTCAAACTGAATAAATACCCCGGAGatcg > 2:1258349/1‑97 (MQ=255)
aaTCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataata > 1:308543/1‑100 (MQ=255)
cGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCt < 1:124006/100‑1 (MQ=255)
gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt > 2:2520800/1‑100 (MQ=255)
gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt > 1:1069706/1‑100 (MQ=255)
aCATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCttt < 1:1459071/100‑1 (MQ=255)
cATCCGTTCCACTGTGCCGGTCAACGGCCCGCTAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAACCTGAATAAATACCCCGGTGAAAATGTCTTTc > 2:2337664/1‑100 (MQ=255)
cATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTc < 1:534223/100‑1 (MQ=255)
aTCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCa < 1:339670/100‑1 (MQ=255)
tCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCttt < 2:1379137/97‑1 (MQ=255)
tCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCAc > 1:37146/1‑100 (MQ=255)
tCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAATGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATATTTc > 2:502639/1‑98 (MQ=255)
ccGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCAcc > 2:1005581/1‑100 (MQ=255)
gTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAg > 1:775294/1‑100 (MQ=255)
tCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCg < 2:2186485/100‑1 (MQ=255)
cACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGAGTTCATACCGTTTTCAAACTGAATAAATACACCGGTGATAATAACTTTCACAAGCGt > 1:671180/1‑99 (MQ=255)
atctccGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGc < 2:868157/96‑1 (MQ=255)
gtgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCg < 2:2441554/100‑1 (MQ=255)
tgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGa < 2:2511096/100‑1 (MQ=255)
gCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaa > 2:864821/1‑100 (MQ=255)
ccGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGcagat > 1:868154/1‑96 (MQ=255)
ccGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAc < 2:2234583/100‑1 (MQ=255)
ccGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAc < 1:2038575/100‑1 (MQ=255)
ggTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCa < 2:2207954/100‑1 (MQ=255)
catcgtccccATAGTCACCAAATATCCAGTGTTCATCCCGTCTTCAAACTGAATAAAGACCCCGTTGATAACATCTTTCACCAGCGTTTGCGAAc < 1:2218995/89‑1 (MQ=255)
cAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAAc > 1:51709/1‑100 (MQ=255)
taCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACg < 1:1256932/99‑1 (MQ=255)
aaCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACg > 2:782766/1‑100 (MQ=255)
aaCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACg > 2:644190/1‑100 (MQ=255)
aaCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACg < 1:1572640/100‑1 (MQ=255)
aCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACga > 2:1256936/1‑100 (MQ=255)
cGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACgag > 1:779080/1‑100 (MQ=255)
ggCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACgag > 2:1232250/1‑99 (MQ=255)
gCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGAt < 2:993688/100‑1 (MQ=255)
cccGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATa < 1:1478489/100‑1 (MQ=255)
cGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGc > 1:1570358/1‑100 (MQ=255)
gATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGcc < 1:2417847/100‑1 (MQ=255)
gATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGcc < 1:909639/100‑1 (MQ=255)
gATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGcc < 2:1344371/100‑1 (MQ=255)
gATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGcc < 2:1964675/100‑1 (MQ=255)
gTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGAc > 2:241263/1‑100 (MQ=255)
gTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGAc > 2:872267/1‑100 (MQ=255)
cACCAAATATCCCGTGTGCATGCCGTTTAGAAACTGAATAAATCCCACGGTGATAATATCTTTCACCAGCTTTAGCGAACCAAACGAGATAGCCAGACCt > 1:2143664/1‑100 (MQ=255)
cACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCt > 1:2019471/1‑100 (MQ=255)
cACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCt > 2:1208648/1‑100 (MQ=255)
cAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAAt > 1:1996797/1‑100 (MQ=255)
|
GAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAAT > NC_000913/843653‑843843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
