Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	14	141.9	7085095	93.3%	6610393	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,928	C→A	L107F (TTG→TTT)	mlaE ←	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,928	0	C	A	99.2%	463.3 / ‑3.5	130	coding (321/783 nt)	mlaE	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (65/64); minor base . (1/0); total (66/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 138 total aligned reads displayed.

GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAG  >  NC_000913/3338831‑3339023

gCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                               <  2:523352/100‑1 (MQ=255)

 cGGTTCCACCGCCATCATCTCCGTTCTGGAGAGGTGCTCTGTAGCGCGCATCAGGCCGATGTCTGCGGTTAGCGCCGATCCAGCACGCCCGGCAaaaaac                                                                                              <  1:146144/100‑1 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              <  1:281259/100‑1 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              <  1:3045622/100‑1 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              >  1:3286646/1‑100 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              >  2:1693289/1‑100 (MQ=255)

      ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                         <  1:2614969/100‑1 (MQ=255)

       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                        >  1:1337840/1‑100 (MQ=255)

        aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                       <  2:891999/100‑1 (MQ=255)

         ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                      <  2:982834/100‑1 (MQ=255)

         ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                      >  2:3234186/1‑100 (MQ=255)

           gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                    >  2:2044470/1‑100 (MQ=255)

            ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                   <  1:771404/100‑1 (MQ=255)

             catcatCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCACGAAAAAAAACAACGCGGCAacc                                                                                  <  1:3095550/100‑1 (MQ=255)

              atcatcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca                                                                                 <  2:1814016/100‑1 (MQ=255)

                 atcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCg                                                                              <  2:1348326/100‑1 (MQ=255)

                  tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                             >  1:1079685/1‑100 (MQ=255)

                   ctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                            >  1:1492862/1‑100 (MQ=255)

                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           >  1:710656/1‑100 (MQ=255)

                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           >  2:2198462/1‑100 (MQ=255)

                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           >  1:1696793/1‑100 (MQ=255)

                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           >  2:561138/1‑100 (MQ=255)

                     ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                          >  2:1194955/1‑100 (MQ=255)

                     ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           <  2:2806226/99‑1 (MQ=255)

                      cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                         <  1:2275549/100‑1 (MQ=255)

                      cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                         >  2:2604210/1‑100 (MQ=255)

                       aTACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCa                                                                        <  1:881534/100‑1 (MQ=255)

                         aCTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGt                                                                      <  1:1085287/100‑1 (MQ=255)

                          cTGGAGAGTTGCTCTGTAGCGCGCATCAAGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                     >  2:1638619/1‑100 (MQ=255)

                            ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                   >  2:1814708/1‑100 (MQ=255)

                             gagaGTTGCTCTGTAGCGCGCATCAAGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCAc                                                                  <  1:1638613/100‑1 (MQ=255)

                              agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                 <  2:654702/100‑1 (MQ=255)

                               gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                <  1:2538009/100‑1 (MQ=255)

                               gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                <  1:3386579/100‑1 (MQ=255)

                               gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGACCCAGTTCACGc                                                                <  2:2315182/100‑1 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACgaga                                                              >  2:583887/1‑97 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  2:1871887/1‑100 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  1:3452097/1‑100 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  1:2433732/1‑100 (MQ=255)

                                  ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGt                                                             <  1:751094/100‑1 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  2:3095616/1‑100 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  2:662392/1‑100 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  1:1991100/1‑100 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  2:419300/1‑100 (MQ=255)

                                      tctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCg                                                         <  2:868401/100‑1 (MQ=255)

                                      tctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCg                                                         <  2:678572/100‑1 (MQ=255)

                                        tGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGAt                                                       >  2:3132785/1‑100 (MQ=255)

                                           aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                    <  1:561136/100‑1 (MQ=255)

                                              gcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcc                                                 <  2:555885/100‑1 (MQ=255)

                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCGACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                              >  2:711657/1‑100 (MQ=255)

                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                              <  1:317907/100‑1 (MQ=255)

                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                              <  1:164071/100‑1 (MQ=255)

                                                  aTCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacca                                             <  2:2844700/100‑1 (MQ=255)

                                                    cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                           >  1:2100510/1‑100 (MQ=255)

                                                    cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                           <  1:2118661/100‑1 (MQ=255)

                                                     aGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                          <  2:1925650/100‑1 (MQ=255)

                                                       gCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAA‑AACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                       >  1:1794345/1‑100 (MQ=255)

                                                        ccGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                       <  1:1871876/100‑1 (MQ=255)

                                                           aTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                    <  1:2983950/100‑1 (MQ=255)

                                                            tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAg                                   >  2:1338846/1‑100 (MQ=255)

                                                              tCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGAAGTAGCGATAACGCCACCAGCATACCCAGa                                  >  2:2952677/1‑99 (MQ=255)

                                                               cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                >  2:3366987/1‑100 (MQ=255)

                                                               cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                <  1:1029185/100‑1 (MQ=255)

                                                                tGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTg                               <  1:1959850/100‑1 (MQ=255)

                                                                 gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTg                               <  2:2257598/99‑1 (MQ=255)

                                                                  cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTg                               >  1:2151755/1‑98 (MQ=255)

                                                                  cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGt                             >  2:2832396/1‑100 (MQ=255)

                                                                   gtttAGCGCCGAAACAGCACGCCCGGCAAAAGACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                            >  2:2377920/3‑100 (MQ=255)

                                                                      tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                         <  2:2866318/100‑1 (MQ=255)

                                                                        gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCGGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                       <  2:1696799/100‑1 (MQ=255)

                                                                         cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                      <  1:389452/100‑1 (MQ=255)

                                                                         cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                      >  1:598219/1‑100 (MQ=255)

                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                     <  2:32233/100‑1 (MQ=255)

                                                                           ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                    >  2:1200210/1‑100 (MQ=255)

                                                                           ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                    <  1:89507/100‑1 (MQ=255)

                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                 <  2:2433750/100‑1 (MQ=255)

                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                 <  1:2373137/100‑1 (MQ=255)

                                                                               aCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATaa                >  1:1884005/1‑100 (MQ=255)

                                                                                ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg               >  1:2710165/1‑100 (MQ=255)

                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt              <  1:622362/100‑1 (MQ=255)

                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt              <  2:14131/100‑1 (MQ=255)

                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt              >  2:2786624/1‑100 (MQ=255)

                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg             >  2:2615284/1‑100 (MQ=255)

                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg             <  1:1198675/100‑1 (MQ=255)

                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTCTCCGCACTATAAGTg             >  1:2295482/1‑100 (MQ=255)

                                                                                    cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGt           <  2:1436705/100‑1 (MQ=255)

                                                                                    cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGt           >  2:728684/1‑100 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  2:965789/1‑100 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  2:929795/1‑100 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  1:2686691/1‑100 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  1:2601129/1‑100 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  1:2456391/1‑100 (MQ=255)

                                                                                         ccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa      >  1:3050246/1‑100 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  2:1492868/100‑1 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  1:2924069/100‑1 (MQ=255)

                                                                                            gCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCa   >  2:233816/1‑100 (MQ=255)

                                                                                             cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg  <  1:1194951/100‑1 (MQ=255)

                                                                                             cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg  >  1:3163997/1‑100 (MQ=255)

                                                                                             cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg  >  1:3233112/1‑100 (MQ=255)

                                                                                             cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg  >  2:116503/1‑100 (MQ=255)

GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAG  >  NC_000913/3338831‑3339023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A