Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F113 I1 R1 18 155.0 7745081 93.4% 7233905 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,338,928 C→A L107F (TTG→TTT)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,9280CA98.5% 447.9 / ‑3.0 130L107F (TTG→TTT) mlaEABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (58/70);  total (60/70)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 136 total aligned reads displayed.

CGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGA  >  NC_000913/3338832‑3339024
                                                                                                |                                                                                                
 ggATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaaca                                                                                              >  1:2522014/1‑100 (MQ=255)
  gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                             <  1:1946257/100‑1 (MQ=255)
  gATCCACCCCCATCATCTGCAGACTGGAGAGTGTCTCGGGAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                             <  2:3577215/100‑1 (MQ=255)
    tCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcg                                                                                           <  2:245969/100‑1 (MQ=255)
     ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                          <  1:1103838/100‑1 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  1:3182212/1‑100 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  1:303442/1‑100 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  1:2647633/1‑100 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  2:662782/1‑100 (MQ=255)
       aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                        >  2:3395539/1‑100 (MQ=255)
       aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                        <  1:1685729/100‑1 (MQ=255)
        ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                       >  2:2053893/1‑100 (MQ=255)
         cGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCa                                                                                      >  2:2088901/1‑100 (MQ=255)
         cGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCa                                                                                      >  1:1070145/1‑100 (MQ=255)
          gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                     >  1:38919/1‑100 (MQ=255)
           ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTGGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                    >  2:1805917/1‑100 (MQ=255)
               cgtctcCATACTGGAGAGTTGCTCTGTAGCGCGAATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccac                                                                                 <  1:3708553/97‑1 (MQ=255)
               catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                                <  1:2296667/100‑1 (MQ=255)
               catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                                <  2:303440/100‑1 (MQ=255)
                ctctccagtatGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCg                                                                               >  1:437013/11‑100 (MQ=255)
                 tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                              <  1:651659/100‑1 (MQ=255)
                 tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGAGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                              >  1:2913279/1‑100 (MQ=255)
                   tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                            >  2:425662/1‑100 (MQ=255)
                    ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                           >  2:2168507/1‑100 (MQ=255)
                     atctcTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAAGCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                          <  2:2120197/96‑1 (MQ=255)
                         cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAgat                                                                      >  1:2120205/1‑98 (MQ=255)
                         cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                      <  1:1031731/100‑1 (MQ=255)
                         cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                      >  2:72923/1‑100 (MQ=255)
                           ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                    <  1:3011759/100‑1 (MQ=255)
                           ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                    >  1:2546234/1‑100 (MQ=255)
                           ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                    <  1:2267721/100‑1 (MQ=255)
                              gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                 <  1:2187438/100‑1 (MQ=255)
                                gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGTGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                               >  2:2244558/1‑100 (MQ=255)
                                gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                               >  2:339406/1‑100 (MQ=255)
                                gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                               >  1:1193922/1‑100 (MQ=255)
                                   gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                            >  2:3515624/1‑100 (MQ=255)
                                   gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                            >  1:3674037/1‑100 (MQ=255)
                                    ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                           <  1:1913606/100‑1 (MQ=255)
                                    ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                           <  2:567573/100‑1 (MQ=255)
                                        gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                       <  2:2913264/100‑1 (MQ=255)
                                        gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                       <  2:573641/100‑1 (MQ=255)
                                          aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                     <  1:3145618/100‑1 (MQ=255)
                                          aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                     <  1:3074324/100‑1 (MQ=255)
                                          aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                     >  2:2871052/1‑100 (MQ=255)
                                           gcgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACg                                                    >  2:2259271/1‑100 (MQ=255)
                                            cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGc                                                   <  1:2943927/100‑1 (MQ=255)
                                             gcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcc                                                  <  1:2267668/100‑1 (MQ=255)
                                               gcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccac                                                <  2:819167/100‑1 (MQ=255)
                                                cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                               <  1:1355136/100‑1 (MQ=255)
                                                   cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                            <  2:3449253/100‑1 (MQ=255)
                                                   cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                            >  1:1203978/1‑100 (MQ=255)
                                                    aGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                           <  2:963576/100‑1 (MQ=255)
                                                     ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                          >  2:1510770/1‑100 (MQ=255)
                                                     ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                          <  2:2486945/100‑1 (MQ=255)
                                                       ccGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                        >  2:3712353/1‑100 (MQ=255)
                                                       ccGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                        >  2:3130330/1‑100 (MQ=255)
                                                         gATTTCTGCGGTTAGCGCCGAACCAGCCCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAcc                                       <  1:2493110/99‑1 (MQ=255)
                                                         gATTTCTGCGCTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGGTCTCGCAGTAGCGATAACGCCACCAGCATAccc                                      <  2:2538981/100‑1 (MQ=255)
                                                          aTTTCTGCGGTTAGCGCCGAACAAACAAAAACAAAAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                     <  2:1853794/100‑1 (MQ=255)
                                                           tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCan                                    <  1:3348833/100‑2 (MQ=255)
                                                           tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAg                                    <  2:2962684/100‑1 (MQ=255)
                                                              cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                 <  2:2996410/100‑1 (MQ=255)
                                                                gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                               <  1:2088908/100‑1 (MQ=255)
                                                                   ggtAGCGCCGAACGAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                            <  2:884405/98‑1 (MQ=255)
                                                                   gTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                            >  2:1686448/1‑100 (MQ=255)
                                                                     tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          <  2:1972386/100‑1 (MQ=255)
                                                                     tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          >  2:2442667/1‑100 (MQ=255)
                                                                      aGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          <  2:3106925/99‑1 (MQ=255)
                                                                      aGCGCCGAACAAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCCCGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTc                           >  2:715578/1‑98 (MQ=255)
                                                                       gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                        <  2:3641529/100‑1 (MQ=255)
                                                                       gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                        >  1:2493445/1‑100 (MQ=255)
                                                                        cgcCGAACCAGCACGCCCGGCAAGAAACAACGCGGCAACCCCCCGCCACAGTTCACTCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          <  2:2024668/97‑1 (MQ=255)
                                                                         gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtagatcgg                       >  2:2895400/1‑92 (MQ=255)
                                                                         gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                      >  1:3748923/1‑100 (MQ=255)
                                                                          ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                     >  1:1205626/1‑100 (MQ=255)
                                                                          ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                     <  2:1263465/100‑1 (MQ=255)
                                                                          ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                     <  2:1422002/100‑1 (MQ=255)
                                                                            gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                   <  1:1964768/100‑1 (MQ=255)
                                                                            gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                   <  1:3712370/100‑1 (MQ=255)
                                                                             attcAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                  >  2:2800017/4‑100 (MQ=255)
                                                                              aCCAGCACGCCCGTCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                  <  2:38919/99‑1 (MQ=255)
                                                                              aCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATaa                 >  2:1939635/1‑100 (MQ=255)
                                                                                cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt               >  2:470900/1‑100 (MQ=255)
                                                                                 aGCACGCCCGGCAAACAACAACGAGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg              >  1:3484185/1‑100 (MQ=255)
                                                                                 aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg              >  2:314465/1‑100 (MQ=255)
                                                                                 aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg              >  2:2573882/1‑100 (MQ=255)
                                                                                  gCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg             <  1:840980/100‑1 (MQ=255)
                                                                                  gCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg             <  2:1205624/100‑1 (MQ=255)
                                                                                    aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc           <  2:3416200/100‑1 (MQ=255)
                                                                                    aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc           <  2:3860055/100‑1 (MQ=255)
                                                                                    aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc           <  2:437012/100‑1 (MQ=255)
                                                                                    aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc           <  2:1070142/100‑1 (MQ=255)
                                                                                     cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa          <  1:878040/100‑1 (MQ=255)
                                                                                     cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa          >  2:2942983/1‑100 (MQ=255)
                                                                                       cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGa        <  2:3643422/100‑1 (MQ=255)
                                                                                        ccGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa       >  2:2950710/1‑100 (MQ=255)
                                                                                        ccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa       >  1:3659890/1‑100 (MQ=255)
                                                                                          ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc     >  1:1430292/1‑100 (MQ=255)
                                                                                            cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg   >  2:2559931/1‑100 (MQ=255)
                                                                                             aaaaaaCAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGa  >  2:2725726/1‑100 (MQ=255)
                                                                                                |                                                                                                
CGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGA  >  NC_000913/3338832‑3339024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A