Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F129 I0 R1 205 159.3 7901478 93.8% 7411586 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,140 A→G 15.9% intergenic (‑191/+38) ibsC ← / ← serA toxic membrane protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1400AG15.9% 484.1 / 74.5 207intergenic (‑191/+38)ibsC/serAtoxic membrane protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (86/87);  new base G (11/22);  total (97/110)
Fisher's exact test for biased strand distribution p-value = 9.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

CAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTT  >  NC_000913/3057041‑3057236
                                                                                                   |                                                                                                
    gCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCa                                                                                              >  2:77126/1‑100 (MQ=255)
     cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                             <  1:1428436/100‑1 (MQ=255)
         gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCATCGCGGCAAGGGTGTGGAGAAGGGATTAAAAAACGGGTGAGTCAggg                                                                                           >  2:2351525/1‑96 (MQ=255)
              gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                    <  1:535258/100‑1 (MQ=255)
              gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                    >  1:3544675/1‑100 (MQ=255)
              gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                    <  1:1348332/100‑1 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                  >  2:2402555/1‑100 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                  >  2:1397777/1‑100 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                  >  1:1533564/1‑100 (MQ=255)
                 tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGt                                                                                 <  1:1402628/100‑1 (MQ=39)
                 tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                 >  2:2958307/1‑100 (MQ=255)
                  ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtt                                                                                <  2:2969625/100‑1 (MQ=39)
                  ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtt                                                                                <  1:2336505/100‑1 (MQ=39)
                  ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtt                                                                                <  1:2716995/100‑1 (MQ=39)
                  ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                <  1:3346706/100‑1 (MQ=255)
                   tGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttt                                                                               <  1:330302/100‑2 (MQ=39)
                   tGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttt                                                                               <  1:1523026/100‑2 (MQ=39)
                   ggacatatttTCGGAAGCAGACACAAAAAGTAGAATGCGCAACGCGGCAACGGTGAAAAAAAAAAAAAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                <  2:1091592/94‑1 (MQ=255)
                     atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                             <  1:1167800/100‑1 (MQ=255)
                     atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                             >  2:1979600/1‑100 (MQ=255)
                     aattatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                             >  1:2672428/3‑100 (MQ=255)
                      tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                            >  1:2604298/1‑100 (MQ=255)
                         atttTTTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGACAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                         <  2:1138013/100‑1 (MQ=255)
                         atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCACCGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                         >  2:1914773/1‑100 (MQ=255)
                         atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                         >  1:1700685/1‑100 (MQ=255)
                          ttttCTGGAGCAGGCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                        >  1:3152565/1‑100 (MQ=255)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACTGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatc                                                                        <  1:436975/100‑9 (MQ=25)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatc                                                                        <  1:3680439/100‑9 (MQ=37)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatc                                                                        <  1:302234/100‑9 (MQ=37)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                        <  1:1251204/100‑1 (MQ=255)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                        <  2:1820315/100‑1 (MQ=255)
                           tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCCGTGACCTGCCCGTTGATTTTCag                                                                       >  2:3080456/1‑100 (MQ=255)
                           tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                       <  2:394161/100‑1 (MQ=255)
                           tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                       <  2:3737535/100‑1 (MQ=255)
                             tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                     <  2:1702397/100‑1 (MQ=255)
                                 gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                 >  1:3113667/1‑100 (MQ=255)
                                  aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                >  2:38266/1‑100 (MQ=255)
                                  aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGCAGAAGGGATAAAAAAACGGGCAGGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                <  2:2462357/100‑1 (MQ=255)
                                    cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                              <  1:1836741/100‑1 (MQ=255)
                                     aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                             >  2:2512402/1‑100 (MQ=255)
                                       aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                           <  1:38266/100‑1 (MQ=255)
                                        cTCGCAAAGTAGACTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                          >  2:2446706/1‑100 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                          <  1:2979896/100‑1 (MQ=255)
                                          cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                        >  2:2716792/1‑100 (MQ=255)
                                           gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                       >  1:2015830/1‑100 (MQ=255)
                                           gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                       >  1:1656972/1‑100 (MQ=255)
                                           gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                       >  2:3921053/1‑100 (MQ=255)
                                           gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                       >  1:550899/1‑100 (MQ=255)
                                            cAAAGTGGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                      <  2:593943/100‑1 (MQ=255)
                                            cAAAGTAGAATGCGCAACGCGGGAACGGTGTTGAGAACGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                      <  2:2667519/100‑1 (MQ=255)
                                             aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                     <  1:2225047/100‑1 (MQ=255)
                                             aaaGTAAAATGAAAAACGCGGTAGCGGTGTAGAGAAAAAAAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                     <  2:677021/100‑1 (MQ=255)
                                              aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                    <  2:541920/100‑1 (MQ=255)
                                               aGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                   <  2:661578/100‑1 (MQ=255)
                                                gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                  <  1:1772246/100‑1 (MQ=255)
                                                gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                  >  2:2623200/1‑100 (MQ=255)
                                                   gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGAc                                               <  1:1748357/100‑1 (MQ=255)
                                                        cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                          >  1:2929995/1‑100 (MQ=255)
                                                        cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                          >  1:1393517/1‑100 (MQ=255)
                                                         gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcgc                                         >  1:2101592/1‑100 (MQ=255)
                                                          cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                        <  1:3706259/100‑1 (MQ=255)
                                                              gcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt                                     >  2:472307/1‑99 (MQ=255)
                                                              gcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                    <  1:3458867/100‑1 (MQ=255)
                                                               cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                   <  2:1291532/100‑1 (MQ=255)
                                                               cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                   <  1:1397786/100‑1 (MQ=255)
                                                                ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGt                                  <  1:1979610/100‑1 (MQ=255)
                                                                 gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTa                                 <  2:3583894/100‑1 (MQ=255)
                                                                  cAACGGTGGGGGGAAGAGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTa                                 <  2:1197142/99‑1 (MQ=255)
                                                                      ggTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                            <  1:713896/100‑1 (MQ=255)
                                                                        tgtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGaa                          <  1:1872549/100‑1 (MQ=255)
                                                                         gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                         >  1:886104/1‑100 (MQ=255)
                                                                         gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                         <  2:1609979/100‑1 (MQ=255)
                                                                           ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                       <  1:3192758/100‑1 (MQ=255)
                                                                           ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                       >  2:3571112/1‑100 (MQ=255)
                                                                              gaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                    <  1:238655/100‑1 (MQ=255)
                                                                                 gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTTTTTTTTTTTGAATTAGTACAGCAGACGGGCGCTAATGGTATCCGTAATAGCTTTCa                 >  2:3626811/1‑100 (MQ=255)
                                                                                   gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATATTCAGAGAAGGGGCATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt               >  2:637217/1‑100 (MQ=255)
                                                                                    aTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg              <  1:2267776/100‑1 (MQ=255)
                                                                                      aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc            >  2:3618305/1‑100 (MQ=255)
                                                                                      aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc            <  2:1574633/100‑1 (MQ=255)
                                                                                       aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt           >  2:2009557/1‑100 (MQ=255)
                                                                                       aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt           >  2:2886708/1‑100 (MQ=255)
                                                                                       aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt           >  1:1684100/1‑100 (MQ=255)
                                                                                       aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt           >  2:3855712/1‑100 (MQ=255)
                                                                                        aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg          >  1:1031953/1‑100 (MQ=255)
                                                                                         tcaaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc         >  2:1899816/3‑100 (MQ=38)
                                                                                         aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc         >  1:821609/1‑100 (MQ=255)
                                                                                         aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc         <  2:3688364/100‑1 (MQ=255)
                                                                                          caaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGCGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa        >  1:3904500/2‑100 (MQ=38)
                                                                                          aaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa        <  2:350757/100‑1 (MQ=255)
                                                                                          aaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa        <  2:3190867/100‑1 (MQ=255)
                                                                                           aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg       <  2:3924684/100‑1 (MQ=255)
                                                                                           aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg       >  1:3477284/1‑100 (MQ=255)
                                                                                            aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAttagatcggaa      >  1:3927706/1‑91 (MQ=38)
                                                                                            aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc      >  1:3257039/1‑100 (MQ=39)
                                                                                            aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc      >  1:3432942/1‑100 (MQ=39)
                                                                                            aCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc      <  2:802290/100‑1 (MQ=255)
                                                                                            aCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc      <  2:1031947/100‑1 (MQ=255)
                                                                                              gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc    >  1:2803614/1‑100 (MQ=255)
                                                                                                gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt  >  1:1066063/1‑100 (MQ=255)
                                                                                                   |                                                                                                
CAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTT  >  NC_000913/3057041‑3057236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A