Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F78 I1 R1 17 139.3 7053113 93.9% 6622873 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,598 G→A G61E (GGA→GAA)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,5980GA98.4% 432.6 / ‑2.6 126G61E (GGA→GAA) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (60/64);  total (61/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 133 total aligned reads displayed.

TGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCA  >  NC_000913/3966502‑3966693
                                                                                                |                                                                                               
tGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAAtt                                                                                              >  2:138327/1‑100 (MQ=255)
  cGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTg                                                                                            <  1:1357465/100‑1 (MQ=255)
   gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCTCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTgg                                                                                           >  2:2482353/1‑100 (MQ=255)
   gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTgg                                                                                           <  1:3439894/100‑1 (MQ=255)
   gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTgg                                                                                           <  2:1527368/100‑1 (MQ=255)
    tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGt                                                                                          <  2:3377909/100‑1 (MQ=255)
    tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGt                                                                                          >  1:3007614/1‑100 (MQ=255)
     aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGtt                                                                                         <  2:1287588/100‑1 (MQ=255)
     aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGtt                                                                                         <  1:2482337/100‑1 (MQ=255)
      aGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGttt                                                                                        <  1:260179/100‑1 (MQ=255)
      aGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGttt                                                                                        >  2:2122697/1‑100 (MQ=255)
      aGCAAGACATTATTTGTGCTATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTAGTGATTGCTTACTGGAGATATTGTAGGATGAATTTgg                                                                                           <  1:2180081/97‑1 (MQ=255)
       gCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtc                                                                                       <  2:585783/100‑1 (MQ=255)
       gCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtc                                                                                       >  2:919953/1‑100 (MQ=255)
        cAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGTGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcc                                                                                      <  1:2813426/100‑1 (MQ=255)
        cAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcc                                                                                      <  1:2935387/100‑1 (MQ=255)
         aGGACATTATTTTTTTTTTTTTTAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcct                                                                                     >  1:3352518/1‑100 (MQ=255)
         aGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcct                                                                                     <  1:679784/100‑1 (MQ=255)
          ggACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcctc                                                                                    <  2:2179012/100‑1 (MQ=255)
          ggACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcctc                                                                                    <  2:2072755/100‑1 (MQ=255)
           gACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcctcc                                                                                   <  2:1427329/100‑1 (MQ=255)
           gACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTtcctcc                                                                                   <  2:1882661/100‑1 (MQ=255)
            aCATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCg                                                                                  >  2:2619768/1‑100 (MQ=255)
               ttattTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTc                                                                               >  2:2857751/1‑100 (MQ=255)
                tattTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTcc                                                                              <  1:2709987/100‑1 (MQ=255)
                   ttttGCCATCCTGAAGCAGCACGCAAAGAATGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATCTTGCAGGATGAATTTGGTTTCCTCCGTTCCGCa                                                                           <  1:2121664/100‑1 (MQ=255)
                    tttGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAg                                                                          <  2:825592/100‑1 (MQ=255)
                    tttGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAg                                                                          <  1:2911141/100‑1 (MQ=255)
                       gCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACa                                                                       >  1:3471353/1‑100 (MQ=255)
                       gCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACa                                                                       <  2:910841/100‑1 (MQ=255)
                         cATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGc                                                                     <  2:2905064/100‑1 (MQ=255)
                          aTCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCt                                                                    <  1:2888432/100‑1 (MQ=255)
                              tGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTa                                                                >  1:1382667/1‑100 (MQ=255)
                                 agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCt                                                             <  2:3007635/100‑1 (MQ=255)
                                   cagcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCg                                                           >  1:2326177/1‑100 (MQ=255)
                                   cagcaCGCAAAGAGTGGCGAAGATATCTGTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCg                                                           <  2:342742/100‑1 (MQ=255)
                                    agcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGc                                                          >  2:2223024/1‑100 (MQ=255)
                                    agcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGc                                                          >  1:74614/1‑100 (MQ=255)
                                     gcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGcc                                                         <  1:112912/100‑1 (MQ=255)
                                      caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCg                                                        >  2:1054343/1‑100 (MQ=255)
                                       aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCgg                                                       <  2:3032028/100‑1 (MQ=255)
                                       aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCgg                                                       <  2:410425/100‑1 (MQ=255)
                                        cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGt                                                      <  2:335079/100‑1 (MQ=255)
                                        cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGt                                                      >  1:2791311/1‑100 (MQ=255)
                                          cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTTGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    <  1:1065658/100‑1 (MQ=255)
                                          cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    >  2:2047828/1‑100 (MQ=255)
                                          cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    >  1:1387175/1‑100 (MQ=255)
                                          cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    <  2:228833/100‑1 (MQ=255)
                                          cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    <  2:1472929/100‑1 (MQ=255)
                                           aaaGTGTGGCGAAGATATCTTTGGTGCGGGCGTACTGGAGATACTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTcc                                                    <  2:3084667/99‑1 (MQ=255)
                                             agagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATGTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCtga                                                 >  1:2107989/1‑100 (MQ=255)
                                               agTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCtgatg                                               <  1:2357006/100‑1 (MQ=255)
                                               agTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCtgatg                                               <  2:2761488/100‑1 (MQ=255)
                                                 tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGAc                                             <  1:296557/100‑1 (MQ=255)
                                                  ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACa                                            >  1:3024687/1‑100 (MQ=255)
                                                  ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACa                                            >  2:236487/1‑100 (MQ=255)
                                                  ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACa                                            >  2:1007116/1‑100 (MQ=255)
                                                  ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACa                                            <  1:919949/100‑1 (MQ=255)
                                                   gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACAt                                           >  1:1753663/1‑100 (MQ=255)
                                                    cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTTCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATc                                          <  2:2469380/100‑1 (MQ=255)
                                                    cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATc                                          >  2:309992/1‑100 (MQ=255)
                                                      aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTa                                        <  2:1881251/100‑1 (MQ=255)
                                                         atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGt                                     >  1:2404716/1‑100 (MQ=255)
                                                          tatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGtt                                    <  1:641381/100‑1 (MQ=255)
                                                          tatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGtt                                    >  1:1196603/1‑100 (MQ=255)
                                                            tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTc                                  >  1:2132938/1‑100 (MQ=255)
                                                             cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTcc                                 >  1:1594448/1‑100 (MQ=255)
                                                              tttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTccc                                >  1:1491180/1‑100 (MQ=255)
                                                              tttGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTccc                                <  2:1974415/100‑1 (MQ=255)
                                                              tttGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTccc                                >  1:3452690/1‑100 (MQ=255)
                                                               ttGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTcccc                               >  2:2171298/1‑100 (MQ=255)
                                                               ttGGTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTcccc                               <  2:115189/100‑1 (MQ=255)
                                                                 ggTGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGAGAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTa                             >  1:2245449/1‑100 (MQ=255)
                                                                   tGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGc                           >  1:168782/1‑100 (MQ=255)
                                                                   tGATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGc                           <  1:2222737/100‑1 (MQ=255)
                                                                    gATGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                          <  1:1054339/100‑1 (MQ=255)
                                                                     aTGGCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCa                         >  1:3352776/1‑100 (MQ=255)
                                                                       ggCGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                       >  2:2204196/1‑100 (MQ=255)
                                                                         cGTACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATc                     >  2:2824438/1‑100 (MQ=255)
                                                                           tACTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccg                   >  1:3237738/1‑100 (MQ=255)
                                                                            aCTGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgc                  >  2:3209957/1‑100 (MQ=255)
                                                                              tGGAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgccg                >  2:457202/1‑100 (MQ=255)
                                                                               ggAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGGCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt               >  1:2248427/1‑100 (MQ=255)
                                                                               ggAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt               >  2:68875/1‑100 (MQ=255)
                                                                               ggAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt               >  2:335600/1‑100 (MQ=255)
                                                                               ggAGATATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCGCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt               >  1:1953975/1‑100 (MQ=255)
                                                                                gagaTATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGtt              >  1:2559526/1‑100 (MQ=255)
                                                                                 agaTATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGttt             <  2:1594457/100‑1 (MQ=255)
                                                                                 agaTATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGttt             >  2:2837042/1‑100 (MQ=255)
                                                                                  gaTATTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTc            >  2:2949414/1‑100 (MQ=255)
                                                                                   atatTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCa           <  1:1529377/100‑1 (MQ=255)
                                                                                   atatTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCa           <  1:1529120/100‑1 (MQ=255)
                                                                                   atatTGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCa           <  1:2857731/100‑1 (MQ=255)
                                                                                       tGCAGGATGAATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCt       >  1:3095723/1‑100 (MQ=255)
                                                                                           ggatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc   >  1:3193333/1‑100 (MQ=255)
                                                                                           ggatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc   >  2:1530139/1‑100 (MQ=255)
                                                                                           ggatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc   >  2:575279/1‑100 (MQ=255)
                                                                                           ggatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc   >  2:1283946/1‑100 (MQ=255)
                                                                                           ggatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc   >  2:2768631/1‑100 (MQ=255)
                                                                                            gatgaatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCgag  >  1:2827401/1‑98 (MQ=255)
                                                                                                |                                                                                               
TGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCA  >  NC_000913/3966502‑3966693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A