Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F112 I0 R1 51 112.5 5500062 95.1% 5230558 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 252,589 G→C 17.4% A97P (GCA→CCA)  yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913252,5890GC17.4% 318.3 / 61.1 121A97P (GCA→CCA) yafOmRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (54/46);  new base C (15/6);  total (69/52)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 133 total aligned reads displayed.

GCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252490‑252688
                                                                                                   |                                                                                                   
gCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaag                                                                                                     >  2:1339904/1‑100 (MQ=255)
cgatctGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaag                                                                                                     <  1:394037/95‑1 (MQ=255)
  aaaTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaagca                                                                                                   <  2:2667405/100‑1 (MQ=255)
   aaTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCAt                                                                                                  >  1:382476/1‑100 (MQ=255)
    aTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATg                                                                                                 >  1:2057447/1‑100 (MQ=255)
    aTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATg                                                                                                 >  1:2057431/1‑100 (MQ=255)
     tGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                >  1:2349410/1‑100 (MQ=255)
      gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaagagatcg                                                                                               >  2:394036/1‑94 (MQ=255)
          ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCt                                                                                           <  1:819537/100‑1 (MQ=255)
           taatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTc                                                                                          >  2:253188/1‑100 (MQ=255)
           taatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTc                                                                                          <  1:2664728/100‑1 (MQ=255)
            aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCa                                                                                         <  2:160784/100‑1 (MQ=255)
            aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCa                                                                                         >  1:253356/1‑100 (MQ=255)
            aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCa                                                                                         <  1:715727/100‑1 (MQ=255)
             atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                        >  2:1403979/1‑100 (MQ=255)
              tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGACGTTTGATGAGCAAGCATGGTTGCTCAtt                                                                                       >  2:1652480/1‑100 (MQ=255)
               ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACTAATTACTAAGCGCATTTGGTATATTGTCAGGGGACGTCAGATGAGCAACCATGCTTGCTCAtca                                                                                      <  2:969903/100‑3 (MQ=255)
               ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                      <  1:2165392/100‑1 (MQ=255)
               ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtca                                                                                      <  2:1542925/100‑3 (MQ=255)
                cATTTCCGCCACGGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaa                                                                                     <  2:2507144/100‑4 (MQ=255)
                 aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                    <  1:1067791/100‑1 (MQ=255)
                 aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaa                                                                                    <  1:1094051/100‑5 (MQ=39)
                      cGCCACAGTTGCGCCAATTCAGCAGCACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAtt                                                                                 >  2:2335134/1‑98 (MQ=255)
                      cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               >  2:2409662/1‑100 (MQ=255)
                       gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                              <  1:2379222/100‑1 (MQ=255)
                       gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                              >  1:817300/1‑100 (MQ=255)
                         cacaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaa                                                                            >  2:2112588/1‑100 (MQ=255)
                          acaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaaa                                                                           >  2:586308/1‑100 (MQ=255)
                           caGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTGTGAAAc                                                                          >  2:1524869/1‑100 (MQ=255)
                             gTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCt                                                                        <  1:970249/100‑1 (MQ=255)
                                gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                     <  1:70720/100‑1 (MQ=255)
                                 cgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAc                                                                    >  2:118593/1‑100 (MQ=255)
                                  gcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAcc                                                                   >  2:2735658/1‑100 (MQ=255)
                                   ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  >  1:1978512/1‑100 (MQ=255)
                                   ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  <  1:2403162/100‑1 (MQ=255)
                                   ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  >  1:501905/1‑100 (MQ=255)
                                   ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  >  1:396539/1‑100 (MQ=255)
                                     aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                >  1:819287/1‑100 (MQ=255)
                                     aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                <  2:870724/100‑1 (MQ=255)
                                      aTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCAt                                                               <  1:457711/100‑1 (MQ=255)
                                      aTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCAt                                                               <  1:634425/100‑1 (MQ=255)
                                      aTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCAt                                                               <  2:2365844/100‑1 (MQ=255)
                                       ttCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCAt                                                               <  2:654844/99‑1 (MQ=255)
                                          agcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAAc                                                           >  1:1096915/1‑100 (MQ=255)
                                           gcagAACGAATGACGAAGCGCATTTGGTATATTCTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATGGCCATTCTGAAACCTGAACCTCATAAACt                                                          >  2:1654090/1‑100 (MQ=255)
                                            cagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTg                                                         <  1:1614170/100‑1 (MQ=255)
                                             agAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTgg                                                        <  1:1900583/100‑1 (MQ=255)
                                              gAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGc                                                       >  1:774213/1‑100 (MQ=255)
                                                aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTc                                                     >  1:307425/1‑100 (MQ=255)
                                                aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTc                                                     >  1:762850/1‑100 (MQ=255)
                                                aCGAATGACGAAGCGCATTTGGTATATTGCCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTc                                                     >  2:823274/1‑100 (MQ=255)
                                                  gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCga                                                   <  2:844540/100‑1 (MQ=255)
                                                   aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                  <  2:1939507/100‑1 (MQ=255)
                                                   aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                  <  1:2723263/100‑1 (MQ=255)
                                                    aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                 >  1:960328/1‑100 (MQ=255)
                                                    aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                 <  2:953244/100‑1 (MQ=255)
                                                      gACGAAGCGCATTTGGTATATTGTCAGGGGTCGTGTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATa                                               <  2:2137410/100‑1 (MQ=255)
                                                      gACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATa                                               <  2:2080708/100‑1 (MQ=255)
                                                       aCGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaa                                              >  1:607393/1‑100 (MQ=255)
                                                        cGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                             >  1:15536/1‑100 (MQ=255)
                                                         gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                            >  2:351619/1‑100 (MQ=255)
                                                          aaGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaa                                           >  1:312516/1‑100 (MQ=255)
                                                                aTTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAAt                                     <  2:334801/100‑1 (MQ=255)
                                                                aTTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAAt                                     <  2:15536/100‑1 (MQ=255)
                                                                 tttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATg                                    <  1:1662450/100‑1 (MQ=255)
                                                                   tGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCTTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAATAACTAAATGCa                                  <  2:1444160/100‑1 (MQ=255)
                                                                    ggTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCAt                                 <  1:1249954/100‑1 (MQ=255)
                                                                        tatTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATaaaa                             <  1:302217/100‑1 (MQ=255)
                                                                           tGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTg                          <  1:1430848/100‑1 (MQ=255)
                                                                            gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAGTCTGAGACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAt                            >  2:684587/1‑97 (MQ=255)
                                                                              caaggcgCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTCAAACCTGAACCTCATAAACTGGCTCGAGATGACAACCAAATGCATAAAATTGGGa                       <  2:1178281/94‑1 (MQ=255)
                                                                              cAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGa                       >  2:2686694/1‑100 (MQ=255)
                                                                               aGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaa                      >  1:2650648/1‑100 (MQ=255)
                                                                                gggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaaa                     >  1:1034291/1‑100 (MQ=255)
                                                                                gggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaaa                     >  1:1538445/1‑100 (MQ=255)
                                                                                   gtcGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                  >  1:2009600/3‑100 (MQ=255)
                                                                                    gCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATgg                 >  1:2379710/1‑100 (MQ=255)
                                                                                     cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                <  1:118258/100‑1 (MQ=255)
                                                                                     cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                >  2:2692829/1‑100 (MQ=255)
                                                                                     atggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                >  1:572641/7‑100 (MQ=38)
                                                                                      gTTTGATGAGCAAGCATGGTTGCTGATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa               <  2:1183956/100‑1 (MQ=255)
                                                                                      gTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa               <  2:1334326/100‑1 (MQ=255)
                                                                                       tttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              <  1:2558935/100‑1 (MQ=255)
                                                                                       ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              >  2:1110216/5‑100 (MQ=39)
                                                                                       ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              >  2:1861273/5‑100 (MQ=39)
                                                                                       ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCAGAAACTGGCCCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                  >  1:2521207/5‑96 (MQ=38)
                                                                                        ttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             <  1:698109/100‑1 (MQ=255)
                                                                                        gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  1:248318/4‑100 (MQ=255)
                                                                                        gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  1:12337/4‑100 (MQ=255)
                                                                                        gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  2:967983/4‑100 (MQ=255)
                                                                                         tgatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGCTAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  1:2463268/1‑100 (MQ=255)
                                                                                         caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTTGGAAAAAGGCAGaa            >  1:446831/3‑100 (MQ=255)
                                                                                         caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  1:2481199/3‑100 (MQ=255)
                                                                                         caatgaGCAACCATGCTTGCTCATTGCCAGTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGCAAATAGGAGaa            >  2:1834577/3‑100 (MQ=255)
                                                                                          aatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACATCATAAACTGGCTTGAGATAAAAACCAAATGCATAAAATTGGGAAAATGGCAGagg           >  2:1382582/2‑98 (MQ=255)
                                                                                                 aagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCg    <  1:2335149/100‑1 (MQ=255)
                                                                                                 aagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCg    <  1:1703552/100‑1 (MQ=255)
                                                                                                  agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc   <  1:1652487/100‑1 (MQ=255)
                                                                                                  agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc   <  1:768151/100‑1 (MQ=255)
                                                                                                  agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc   >  2:1318218/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252490‑252688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A