Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F52 I0 R1	129	152.3	7640533	93.0%	7105695	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	252,589	G→C	17.3%	A97P (GCA→CCA)	yafO →	mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	252,589	0	G	C	17.3%	350.7 / 61.8	134	A97P (GCA→CCA)	yafO	mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base G (57/53); new base C (11/12); total (69/65)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 143 total aligned reads displayed.

AAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252492‑252688

   tGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                >  1:1499181/1‑100 (MQ=255)

    gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCTTTTGGTATATTGTCAGGGGGCGTTTGCTGAGCAAGCATGGt                                                                                               <  1:2076389/100‑1 (MQ=255)

    gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATgct                                                                                               <  2:2562550/100‑3 (MQ=255)

      gaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTg                                                                                             >  1:3813669/1‑100 (MQ=255)

       aataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGc                                                                                            <  1:2791306/100‑1 (MQ=255)

        ataatCCATTTCCGCCACAGTTGCGTCAATTCAGCAGAACGAATGACGAAGCGCATTTAGTATATTGTCAGGGGGCGTTTGATGAGCAACCAtgcttgct                                                                                           <  1:317980/100‑1 (MQ=255)

          aatCCATGTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCa                                                                                         <  1:1529786/100‑1 (MQ=255)

           atCCATTTCCGCCACAGTTGCGCTAATTCAGCAGAACGAATGACGAAGCTCATTTGGTATATTGTCAGGGGGCGTTTGATGAGAAAGTATGGTTGCTcct                                                                                        >  1:664941/1‑98 (MQ=255)

           atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                        >  2:903319/1‑100 (MQ=255)

           atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                        >  1:667802/1‑100 (MQ=255)

           atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAt                                                                                        <  2:1872853/100‑1 (MQ=255)

            tcctcttCCGGCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:3443497/95‑2 (MQ=255)

            tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:629361/100‑2 (MQ=255)

            tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:3116413/100‑2 (MQ=255)

            tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:2202992/100‑2 (MQ=255)

             ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                      <  2:790876/100‑1 (MQ=255)

             ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtca                                                                                      <  1:1694948/100‑3 (MQ=255)

             ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtca                                                                                      <  2:261357/100‑3 (MQ=255)

              cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     >  2:1846934/1‑100 (MQ=255)

              cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     <  2:951681/100‑1 (MQ=255)

              cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     <  1:1332978/100‑1 (MQ=255)

              cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     <  1:1777838/100‑1 (MQ=255)

               aTTTCCGCCACGGTTGCGCGAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTGTGATGAGCAACCATGCTTGCTCAtca                                                                                      <  1:1111526/98‑3 (MQ=255)

               aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                    <  1:1008037/100‑1 (MQ=255)

                tttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCa                                                                                   <  2:676435/100‑1 (MQ=255)

                 ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAt                                                                                  >  2:3498877/1‑100 (MQ=255)

                 ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAt                                                                                  >  1:2046308/1‑100 (MQ=255)

                 ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAt                                                                                  <  1:2839864/100‑1 (MQ=255)

                 ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAt                                                                                  >  1:422314/1‑100 (MQ=255)

                 ttCCGCCACAGGTACGCCAATTAAGCAGAACGAATGACGAATCGCATTTGGTATATGGTCAGGGGGCGTTTGATCATCAACCATGCTTGCTCAtc                                                                                       <  1:322176/95‑2 (MQ=255)

                   ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                <  1:3220461/100‑1 (MQ=255)

                    cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               >  2:1035703/1‑100 (MQ=255)

                    cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               >  1:1481663/1‑100 (MQ=255)

                    cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATGTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               <  2:1407448/100‑1 (MQ=255)

                     gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCGATtgtg                                                                              >  1:1340614/1‑100 (MQ=255)

                     gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                              >  2:1477266/1‑100 (MQ=255)

                     gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                              >  1:1280639/1‑100 (MQ=255)

                          aGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAcc                                                                         <  2:3205860/100‑1 (MQ=255)

                          aGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAcc                                                                         <  1:532928/100‑1 (MQ=255)

                          aGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAcc                                                                         <  1:111039/100‑1 (MQ=255)

                          aGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAcc                                                                         <  1:3068833/100‑1 (MQ=255)

                           gTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCt                                                                        >  2:2254179/1‑100 (MQ=255)

                           gTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCt                                                                        >  2:3012416/1‑100 (MQ=255)

                              gcgcCAATTGAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                     <  1:2422499/100‑1 (MQ=255)

                                 ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  >  2:2650620/1‑100 (MQ=255)

                                   aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                <  2:247614/100‑1 (MQ=255)

                                   aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                >  2:1329980/1‑100 (MQ=255)

                                         gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                          <  2:2343870/100‑1 (MQ=255)

                                         gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                          <  2:2857965/100‑1 (MQ=255)

                                         gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                          <  2:3098582/100‑1 (MQ=255)

                                          cagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTg                                                         <  2:3018382/100‑1 (MQ=255)

                                          cagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTg                                                         >  2:1498666/1‑100 (MQ=255)

                                           agAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTgg                                                        <  1:10377/100‑1 (MQ=255)

                                            gAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGc                                                       >  1:364418/1‑100 (MQ=255)

                                                gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCga                                                   >  2:2118551/1‑100 (MQ=255)

                                                gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCga                                                   >  1:2951009/1‑100 (MQ=255)

                                                  aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                 >  1:211840/1‑100 (MQ=255)

                                                  aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                 <  2:1734706/100‑1 (MQ=255)

                                                       gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                            <  2:2951023/100‑1 (MQ=255)

                                                       gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                            <  2:1850608/100‑1 (MQ=255)

                                                        aaGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaa                                           >  2:1900197/1‑100 (MQ=255)

                                                         aagggaTTTGGGATATTGTTAGGGGGCGTTTGAAGAGCCAGCATGGTTGCTCATTGCCATTTTGAAACCTGAACCTCCAAAACTGGCTCGAGATaaaa                                            <  1:1509993/93‑3 (MQ=255)

                                                          gcgcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                          <  2:27981/99‑1 (MQ=255)

                                                            gcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaa                                       >  1:1232561/1‑100 (MQ=255)

                                                             cATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTGTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaaa                                      >  1:571249/1‑100 (MQ=255)

                                                             cATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaaa                                      >  2:1117229/1‑100 (MQ=255)

                                                                  ggTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCAt                                 <  1:2840117/100‑1 (MQ=255)

                                                                  ggTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCAt                                 <  2:215387/100‑1 (MQ=255)

                                                                     atatTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATaaa                              >  2:3522880/1‑100 (MQ=255)

                                                                          gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                         >  2:2779369/1‑100 (MQ=255)

                                                                          gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                         <  2:1256238/100‑1 (MQ=255)

                                                                          gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTAAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                         <  1:3516506/100‑1 (MQ=255)

                                                                           tCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                        <  1:3717852/100‑1 (MQ=255)

                                                                             aGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaa                      <  2:2336684/100‑1 (MQ=255)

                                                                             aGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaa                      >  1:720237/1‑100 (MQ=255)

                                                                                 ggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                  <  2:1615100/100‑1 (MQ=255)

                                                                                 ggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                  >  2:739582/1‑100 (MQ=255)

                                                                                   cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                >  2:217064/1‑100 (MQ=255)

                                                                                   cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                <  1:105119/100‑1 (MQ=255)

                                                                                     tttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              <  1:2523537/100‑1 (MQ=255)

                                                                                     ttggatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              <  1:2269131/97‑1 (MQ=255)

                                                                                     ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              >  1:1442658/5‑100 (MQ=39)

                                                                                      ttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  1:3102252/1‑100 (MQ=255)

                                                                                      gcaatgaGCAACCATGCTTGCTTATCGCCATTCTGGACATTGAACCGCATAAACTGGCTCGAGATAACAACCCAATGAATAAAATTAGGAAAATGGCAGa             >  1:177422/4‑100 (MQ=255)

                                                                                      gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAAGGTGAACCTCATAAACTGGCTCGAGATAACAACCCAATGCATAAAATTGGGCAAATGGCAGa             >  1:2359120/4‑100 (MQ=255)

                                                                                      gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  1:3726203/4‑100 (MQ=255)

                                                                                      gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  2:3220373/4‑100 (MQ=255)

                                                                                       tgatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  2:264605/1‑100 (MQ=255)

                                                                                       tgatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  1:3689274/1‑100 (MQ=255)

                                                                                       caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  1:2553049/3‑100 (MQ=255)

                                                                                       caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  2:195469/3‑100 (MQ=255)

                                                                                          tgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCg         >  2:796700/1‑100 (MQ=255)

                                                                                           gaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGt        >  2:3053621/1‑100 (MQ=255)

                                                                                           gaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGt        >  1:3743933/1‑100 (MQ=255)

                                                                                              caagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTc     >  1:2541774/1‑100 (MQ=255)

                                                                                              caagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTc     <  2:211844/100‑1 (MQ=255)

                                                                                              caagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTc     <  1:802219/100‑1 (MQ=255)

                                                                                               aagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCg    <  2:2446578/100‑1 (MQ=255)

                                                                                                agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc   >  2:3813151/1‑100 (MQ=255)

                                                                                                 gcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCa  <  1:2555053/100‑1 (MQ=255)

AAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252492‑252688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A