Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F55 I0 R1 245 145.6 7293262 93.0% 6782733 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 252,589 G→C 17.5% A97P (GCA→CCA)  yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913252,5890GC17.5% 311.6 / 57.9 124A97P (GCA→CCA) yafOmRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (55/47);  new base C (9/13);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 134 total aligned reads displayed.

GCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252490‑252688
                                                                                                   |                                                                                                   
  aaaTGAGAATAATCCATTTCCGTCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATACTGTCAGGGGGCGTTTGATGagcaagca                                                                                                   <  2:2455815/100‑1 (MQ=255)
     tGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                >  1:2787586/1‑100 (MQ=255)
        gaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTg                                                                                             >  1:1516830/1‑100 (MQ=255)
        gaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTg                                                                                             >  2:3145727/1‑100 (MQ=255)
         aataatCCATTTCCGCCACAGTTGCGCAAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGc                                                                                            <  2:2063488/100‑1 (MQ=255)
          ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCCAGCATGGTTGCt                                                                                           <  1:2392637/100‑1 (MQ=255)
          ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCt                                                                                           <  2:955856/100‑1 (MQ=255)
          ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCt                                                                                           <  1:2538868/100‑1 (MQ=255)
             atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                        >  1:1446018/1‑100 (MQ=255)
              tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAtt                                                                                       >  1:3386919/1‑100 (MQ=255)
              tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:527331/100‑2 (MQ=255)
              tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                       <  1:2349235/100‑2 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     <  1:1703815/100‑1 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     >  1:529992/1‑100 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                     >  1:88661/1‑100 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaa                                                                                     <  1:3204621/100‑4 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaa                                                                                     <  1:3069751/100‑4 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGAGGAGCAAGCATGGTTGCTCATTGc                                                                                     >  2:2210729/1‑100 (MQ=255)
                cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTCTCAGCCAGCGTGTGATGAGCAAGCATGGTTGCTCATTg                                                                                      >  2:2560326/1‑99 (MQ=255)
                 aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                    >  2:225102/1‑100 (MQ=255)
                 aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                    <  1:780437/100‑1 (MQ=255)
                 aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaa                                                                                    <  1:905992/100‑5 (MQ=39)
                  tttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaac                                                                                   <  1:3519290/100‑6 (MQ=39)
                   ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTAATGAGCAACCATGCTTGCTCAtcaaacg                                                                                  <  1:1535392/100‑7 (MQ=38)
                    tCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGACGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAtt                                                                                 >  2:795823/1‑100 (MQ=255)
                     ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTTATGAGCAACCATGCTTGCTCAtcaaa                                                                                    <  2:1354287/96‑5 (MQ=39)
                     ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                >  1:1933792/1‑100 (MQ=255)
                      cGCCACAGTTGCGCGAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               <  2:1567115/100‑1 (MQ=255)
                      cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                               >  1:2329575/1‑100 (MQ=255)
                       gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                >  1:270055/1‑98 (MQ=255)
                       gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                              >  2:2366023/1‑100 (MQ=255)
                         cacaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaa                                                                            >  1:2822639/1‑100 (MQ=255)
                              ttGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTg                                                                       <  2:564652/100‑1 (MQ=255)
                               tGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGa                                                                      <  2:2055210/100‑1 (MQ=255)
                                gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                     >  1:1648933/1‑100 (MQ=255)
                                gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                     >  1:3086024/1‑100 (MQ=255)
                                gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                     >  1:2292761/1‑100 (MQ=255)
                                gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGa                                                                      <  2:2054836/99‑1 (MQ=255)
                                  gcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATAAACGAGGATGGTCTCTACTTGCCAATCGGACACCTTAAc                                                                    >  2:243159/1‑99 (MQ=255)
                                   ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                  >  2:1163102/1‑100 (MQ=255)
                                     aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                <  2:1105841/100‑1 (MQ=255)
                                     aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                >  1:1805815/1‑100 (MQ=255)
                                           gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                          >  2:1390163/1‑100 (MQ=255)
                                              gAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGc                                                       <  1:3181892/100‑1 (MQ=255)
                                               aaCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCt                                                      <  1:1271189/100‑1 (MQ=255)
                                               aaCGAATGACGAAGCGCATTTGCTATATTGTCAGGGGGCGTTTGATGTGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCt                                                      <  2:2456882/100‑1 (MQ=255)
                                                 cGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCg                                                    <  2:3484232/100‑1 (MQ=255)
                                                  gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCga                                                   >  2:2199152/1‑100 (MQ=255)
                                                   aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                  <  1:2288624/100‑1 (MQ=255)
                                                   aaTGACGAAGCGCATTTGGTATATGGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                  <  2:602591/100‑1 (MQ=255)
                                                    tctaCGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                 <  1:2024194/97‑1 (MQ=255)
                                                     tGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGAt                                                <  2:2792721/100‑1 (MQ=255)
                                                     tGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGAt                                                <  2:2627535/100‑1 (MQ=255)
                                                      gACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATa                                               <  2:1425421/100‑1 (MQ=255)
                                                      gACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATa                                               <  2:3068955/100‑1 (MQ=255)
                                                      gACGAAGCGCATTTGCTATATTGTCAGGGGACGATTGATGAGCAAGCATGCTTGCTCATTGACATTCTGAAACCTGAACCTCATAAATTGACTCGAGAt                                                >  2:307583/1‑99 (MQ=255)
                                                        cGAAGCGCATTTGGTATATTGTGAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                             >  2:1205916/1‑100 (MQ=255)
                                                        cGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                             <  1:768707/100‑1 (MQ=255)
                                                         gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                            <  1:3163259/100‑1 (MQ=255)
                                                         gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                            <  1:851934/100‑1 (MQ=255)
                                                           aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCCTTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                             <  2:2252237/97‑1 (MQ=255)
                                                           aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                          >  1:1865307/1‑100 (MQ=255)
                                                           aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGACTCGAGATaacaac                                          <  1:113229/100‑1 (MQ=255)
                                                             cgcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCa                                        >  1:2353574/1‑100 (MQ=255)
                                                              gcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaa                                       >  2:213922/1‑100 (MQ=255)
                                                               cATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaaa                                      <  2:2239871/100‑1 (MQ=255)
                                                               cATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaaa                                      <  2:2240569/100‑1 (MQ=255)
                                                                 tttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATg                                    <  2:3348607/100‑1 (MQ=255)
                                                                  ttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGc                                   >  2:3618188/1‑100 (MQ=255)
                                                                  ttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGc                                   <  2:1648925/100‑1 (MQ=255)
                                                                     ttataTTGTCAGGTGTCGTTGTATGGGCATGCATGGTTTCTCATTGCCAGGCTGTAAGTTGAACCTCATAAACGGGCTCGAGATAACAACCAAAt                                     >  2:3039690/2‑95 (MQ=255)
                                                                     gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                <  1:2159988/100‑1 (MQ=255)
                                                                     gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                >  2:2806248/1‑100 (MQ=255)
                                                                      tataTTGTCAGGGCGCGTTTGACGAGCAAGCATGGTTGCTCATTCACCTTCTGAAACCTGATCCTCATCAACTGCTTCGAGATAACACCCAAATg                                    <  2:3456096/95‑1 (MQ=255)
                                                                         atTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATCCATAAAAt                            >  2:3469482/1‑100 (MQ=255)
                                                                            gTCAGGGGGTGTTTGATGAGCAAGCAGGTGTGCTCATTGCCATTCTCAAACCTGAACATCATAAACTGTCTCGAGATAACAACCAAATGCATAAAATTgg                         <  2:376113/100‑1 (MQ=255)
                                                                            gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                         >  2:3584485/1‑100 (MQ=255)
                                                                            atctgggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                         <  2:3242442/96‑1 (MQ=255)
                                                                             tCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                        <  1:2206338/100‑1 (MQ=255)
                                                                             tCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                        <  1:12388/100‑1 (MQ=255)
                                                                                gggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                        <  1:2158048/97‑1 (MQ=255)
                                                                                gggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaaa                     >  1:588290/1‑100 (MQ=255)
                                                                                  gggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGCGATAACAACCAAATGCATAAAATTGGGTAAAt                   >  1:3438750/1‑100 (MQ=255)
                                                                                      gTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa               >  2:410332/1‑100 (MQ=255)
                                                                                       tttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              <  2:73301/100‑1 (MQ=255)
                                                                                       ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg              >  1:3472581/5‑100 (MQ=39)
                                                                                        ttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCACAAAATTTGGAAAATGGCAGa             >  2:1618404/1‑100 (MQ=255)
                                                                                        gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa             >  2:107074/4‑100 (MQ=255)
                                                                                         tgatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAAACAAATGCATAAAATTGGGAAAATGGCAGaa            >  2:2683882/1‑100 (MQ=255)
                                                                                         caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa            >  1:2770981/3‑100 (MQ=255)
                                                                                          gatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg           >  1:494517/1‑100 (MQ=255)
                                                                                          aatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg           >  2:214427/2‑100 (MQ=255)
                                                                                          aatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg           >  1:782987/2‑100 (MQ=255)
                                                                                           atgaGCAACCATGCTTGCTCATTGGCATGCTGAAACCTGAACCTCATAAACTGGCGCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGc          >  2:1209812/1‑100 (MQ=255)
                                                                                            tgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCg         >  1:355683/1‑100 (MQ=255)
                                                                                            tgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCg         >  2:880221/1‑100 (MQ=255)
                                                                                               gcaagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGttt      >  2:747147/1‑100 (MQ=255)
                                                                                                caagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTc     <  1:241042/100‑1 (MQ=255)
                                                                                                  agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCACATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGTAGAAGCGTTTCGc   >  1:2565700/1‑100 (MQ=255)
                                                                                                   gcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCa  <  2:2745063/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCA  >  NC_000913/252490‑252688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A