Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F112 I0 R1 51 112.5 5500062 95.1% 5230558 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,248,180 A→C 62.4% R136S (AGA→AGC)  yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,248,1800AC62.4% 104.8 / 98.1 98R136S (AGA→AGC) yqjAgeneral envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (13/24);  new base C (26/35);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGATCCTG  >  NC_000913/3248083‑3248276
                                                                                                 |                                                                                                
ctcATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCac                                                                                                <  1:1563894/100‑1 (MQ=255)
  cATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTTTTTTTTTTTTTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAacac                                                                                              >  2:951339/1‑100 (MQ=255)
    tttACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACActg                                                                                            <  1:1196324/100‑1 (MQ=255)
     ttACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACAct                                                                                             <  2:1529861/98‑1 (MQ=255)
       aCCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACActgctg                                                                                         <  1:1137081/100‑1 (MQ=255)
        cccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACtgctgc                                                                                        >  2:1157105/1‑100 (MQ=255)
        cccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACtgctgc                                                                                        <  1:422933/100‑1 (MQ=255)
        cccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACtgctgc                                                                                        >  2:2461713/1‑100 (MQ=255)
        cccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACtgctgc                                                                                        >  1:1635003/1‑100 (MQ=255)
        cccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCATTATTGCGTTTGTCAGCACACtgctgc                                                                                        >  2:594570/1‑100 (MQ=255)
         ccGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGcc                                                                                       >  2:2224948/1‑100 (MQ=255)
            cgcATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCcgac                                                                                    <  1:2646854/100‑1 (MQ=255)
               cgatctatcaACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGAt                                                                                 <  1:1435057/94‑1 (MQ=255)
               aTTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGAt                                                                                 <  1:624864/100‑1 (MQ=255)
               aTTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGAt                                                                                 <  1:1603077/100‑1 (MQ=255)
               aTTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGAt                                                                                 <  2:1634996/100‑1 (MQ=255)
                ttATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGAtt                                                                                >  2:16810/1‑100 (MQ=255)
                 tATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTg                                                                               <  2:502111/100‑1 (MQ=255)
                   tcatcaACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGc                                                                              >  2:457064/1‑99 (MQ=255)
                    catcaACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCg                                                                            >  2:165280/1‑100 (MQ=255)
                     atcaACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGAtagatcg                                                                           >  2:1435051/1‑94 (MQ=255)
                      tcaACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCggg                                                                          >  2:1191675/1‑100 (MQ=255)
                       caACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGt                                                                         >  2:313644/1‑100 (MQ=255)
                        aaCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGtt                                                                        >  2:444984/1‑100 (MQ=255)
                        aaCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGtt                                                                        <  1:1836312/100‑1 (MQ=255)
                        aaCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGtt                                                                        <  1:1564798/100‑1 (MQ=255)
                        aaCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGtt                                                                        <  2:1126255/100‑1 (MQ=255)
                         aCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTa                                                                       <  1:443283/100‑1 (MQ=255)
                         aCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTa                                                                       >  2:931956/1‑100 (MQ=255)
                         aCGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTa                                                                       <  2:345760/100‑1 (MQ=255)
                          cgcgCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTAt                                                                      <  2:2578795/100‑1 (MQ=255)
                               acacCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAggg                                                                 <  2:975447/100‑1 (MQ=255)
                                 acCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCt                                                               <  1:563416/100‑1 (MQ=255)
                                 acCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAACCAATCGCATTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGc                                                                >  2:604126/1‑99 (MQ=255)
                                   cATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGa                                                             <  2:429075/100‑1 (MQ=255)
                                   cATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGa                                                             >  1:1571136/1‑100 (MQ=255)
                                    aTCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGGCGCTTTATTGCGGTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGaa                                                            <  2:330026/100‑1 (MQ=255)
                                      cTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATa                                                          >  2:1418171/1‑100 (MQ=255)
                                        ttttCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATa                                                          >  2:1669567/1‑98 (MQ=255)
                                          ttCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgc                                                      >  2:2214024/1‑100 (MQ=255)
                                           tCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcg                                                     >  1:2730144/1‑100 (MQ=255)
                                           tCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcg                                                     >  1:2620415/1‑100 (MQ=255)
                                            cATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcgc                                                    >  2:1870879/1‑100 (MQ=255)
                                            cATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcgc                                                    >  1:458275/1‑100 (MQ=255)
                                            cATAAACACGGTTTAACGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGACGGGTTATCAGGGCTGAATAAcgcgc                                                    >  2:1870868/1‑100 (MQ=255)
                                             ttAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGTCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcgcg                                                   <  2:1474974/99‑1 (MQ=255)
                                              tAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAAcgcgcga                                                  >  1:1474980/1‑99 (MQ=255)
                                                aaCACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCtt                                                <  1:2427397/100‑1 (MQ=255)
                                                   acGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCa                                             <  2:1940971/100‑1 (MQ=255)
                                                   acGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCa                                             <  1:185484/100‑1 (MQ=255)
                                                    cGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAg                                            >  1:2532600/1‑100 (MQ=255)
                                                    cGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAg                                            <  1:462174/100‑1 (MQ=255)
                                                     ggTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGt                                           >  2:1312283/1‑100 (MQ=255)
                                                     ggTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGt                                           >  2:756546/1‑100 (MQ=255)
                                                     ggTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGt                                           >  2:624871/1‑100 (MQ=255)
                                                     ggTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGt                                           <  2:937869/100‑1 (MQ=255)
                                                        ttATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGtttt                                        <  1:1413603/100‑1 (MQ=255)
                                                        ttATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGtttt                                        <  2:532620/100‑1 (MQ=255)
                                                        ttATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCAAACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGtttt                                        <  2:227320/100‑1 (MQ=255)
                                                        ttATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGtttt                                        <  2:1859017/100‑1 (MQ=255)
                                                          aTCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTc                                      <  1:513776/100‑1 (MQ=255)
                                                          aTCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTc                                      <  2:1396998/100‑1 (MQ=255)
                                                          aTCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTc                                      <  2:603272/100‑1 (MQ=255)
                                                          aTCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTCTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCCTTCTGTTTTTc                                      <  2:1446073/100‑1 (MQ=255)
                                                           tCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGGATAACGCGCGCTTTCAGTTTTTCa                                     <  2:2050631/100‑1 (MQ=255)
                                                           tCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCa                                     <  2:1871488/100‑1 (MQ=255)
                                                           tCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCa                                     <  1:20837/100‑1 (MQ=255)
                                                           tCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCa                                     <  2:1035566/100‑1 (MQ=255)
                                                           tCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCa                                     <  1:1698136/100‑1 (MQ=255)
                                                               cgcTGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTg                                 <  1:945350/100‑1 (MQ=255)
                                                               cgcTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTg                                 <  2:1825049/100‑1 (MQ=255)
                                                                  tGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGAt                              >  1:1578125/1‑100 (MQ=255)
                                                                  tGTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGAt                              <  1:165280/100‑1 (MQ=255)
                                                                   gTTAATTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGTGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATg                             <  1:123835/100‑1 (MQ=255)
                                                                   gTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATg                             <  1:952874/100‑1 (MQ=255)
                                                                      aaTTGGTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGc                          <  1:2325107/100‑1 (MQ=255)
                                                                          ggTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTCAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTc                      >  2:2051345/1‑100 (MQ=255)
                                                                           gTCGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTct                     >  2:2008197/1‑100 (MQ=255)
                                                                           gTCGCTTTATTGCGGTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTc                      >  2:1662721/1‑99 (MQ=255)
                                                                             cGCTTTATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTctgc                   <  2:2532582/100‑1 (MQ=255)
                                                                             cGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTctgc                   >  2:1219226/1‑100 (MQ=255)
                                                                             cGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTctgc                   <  1:554879/100‑1 (MQ=255)
                                                                                 ttATTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCtgtg               <  1:787146/100‑1 (MQ=255)
                                                                                 ttATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCtgtg               <  1:1187803/100‑1 (MQ=255)
                                                                                  tATTGCGTTTGTCAGCATACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTgg              <  1:1781219/100‑1 (MQ=255)
                                                                                  tATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTgg              <  2:1220378/100‑1 (MQ=255)
                                                                                  tATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTgg              <  2:2008571/100‑1 (MQ=255)
                                                                                   aTTGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTggg             <  1:1545174/100‑1 (MQ=255)
                                                                                    ttGCGTTTGTCAGCACAGTGCTGCCGACGATTGCAGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGt            >  2:183913/1‑100 (MQ=255)
                                                                                     tGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTa           >  2:1389425/1‑100 (MQ=255)
                                                                                     tGCGTTTGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTa           >  2:894612/1‑100 (MQ=255)
                                                                                        gTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTg        <  2:2124999/100‑1 (MQ=255)
                                                                                         tttGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGa       >  2:2683005/1‑100 (MQ=255)
                                                                                          ttGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGAt      <  2:2594359/100‑1 (MQ=255)
                                                                                           tGTCAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGATc     <  1:964622/100‑1 (MQ=255)
                                                                                            ctCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGa       <  1:1639884/96‑1 (MQ=255)
                                                                                              cAGCACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGATCCTg  >  2:1856293/1‑100 (MQ=255)
                                                                                              cAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGATCCTg  <  2:182722/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTTTATCGGCGCTGTTAATTGGTCGCTTTATTGCGTTTGTCAGAACACTGCTGCCGACGATTGCCGGGTTATCAGGGCTGAATAACGCGCGCTTTCAGTTTTTCAACTGGATGAGCGGTCTGCTGTGGGTATTGATCCTG  >  NC_000913/3248083‑3248276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A