Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F125 I1 R1
|
17 |
96.7 |
4814547 |
93.9% |
4520859 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,072,171 |
C→A |
V161F (GTC→TTC) |
yihT ← |
6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,072,171 | 0 | C | A | 96.2%
| 274.7
/ ‑1.1
| 78 | V161F (GTC→TTC) | yihT | 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (42/33); total (44/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCC > NC_000913/4072075‑4072266
|
tGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg < 2:872926/100‑1 (MQ=255)
tGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg < 2:1546843/100‑1 (MQ=255)
gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGc < 1:132301/100‑1 (MQ=255)
tAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCt < 2:2224505/100‑1 (MQ=255)
agagGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTc > 2:406845/1‑100 (MQ=255)
ggTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAat < 1:262848/100‑1 (MQ=255)
ggTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAat > 2:40131/1‑100 (MQ=255)
ggTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCAGACGAATGGCTCAat > 1:2364607/1‑100 (MQ=255)
cAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATTGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatgat < 1:2344764/100‑1 (MQ=255)
cgcCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCt < 2:2183076/100‑1 (MQ=255)
ccACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTa < 1:2028074/100‑1 (MQ=255)
cACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTAc < 1:1543250/100‑1 (MQ=255)
aCTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTAcc > 2:107652/1‑100 (MQ=255)
aCTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTAcc < 1:1206167/100‑1 (MQ=255)
tGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGCCGGACGAATGGCTCAATGATGCTTACCAg > 2:829186/1‑100 (MQ=255)
gTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGa > 1:1739604/1‑100 (MQ=255)
gCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCg > 1:1884339/1‑100 (MQ=255)
gCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCg < 2:2252662/100‑1 (MQ=255)
cccAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCgt > 1:2237731/1‑100 (MQ=255)
tctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGt < 2:1514859/100‑1 (MQ=255)
ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTg < 2:1178560/100‑1 (MQ=255)
gatctcggcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCGATGATGCTTACCAGACCGTGTGAGTgg < 1:182819/95‑1 (MQ=255)
ttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGc < 2:2187592/100‑1 (MQ=255)
ttGGCGGCATCGATGATCGCTTGTCCGCGATCGAATTTATCGCCACGACGCGGTGGGTGGACGACTGGCTCAATGAGGCTTACCAGTCCGTGTGAGTg < 2:2065906/98‑1 (MQ=255)
cggcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCGATGATGCTTACCAGACCGTGTGAGTggagatc > 2:182818/1‑95 (MQ=255)
ggcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGt < 2:861781/100‑1 (MQ=255)
cATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTc > 1:547315/1‑100 (MQ=255)
aTCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGAACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCg > 1:2300808/1‑100 (MQ=255)
cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt > 2:2213305/1‑100 (MQ=255)
cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt < 2:977978/100‑1 (MQ=255)
cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt < 1:10143/100‑1 (MQ=255)
cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt > 1:279275/1‑100 (MQ=255)
gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTg < 1:618411/100‑1 (MQ=255)
gatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAc > 2:1597133/1‑100 (MQ=255)
atCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAct < 1:2233740/100‑1 (MQ=255)
cTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCttt > 1:1007070/1‑100 (MQ=255)
tCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCa > 2:517031/1‑100 (MQ=255)
cgATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCatat > 1:1084550/1‑100 (MQ=255)
naTCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTAACCAGACCGTGTGAGAGGCACAGTACGTTGAACTCTTTCACCATATc > 2:1618871/2‑100 (MQ=255)
gATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATc < 2:1022956/100‑1 (MQ=255)
tCGAATTTATCGCCACGACGCGGTGGGCGGACGTATGGCTTAATGATGCTTACCAGGCCGTGTGAGTGGCACAGTTCGTTGAACTCTCTCAACATATcc < 2:1265274/99‑1 (MQ=255)
tCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCa < 1:574048/100‑1 (MQ=255)
cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAg > 1:1702006/1‑100 (MQ=255)
cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAg > 2:1999616/1‑100 (MQ=255)
cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAg > 2:1565677/1‑100 (MQ=255)
gAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGa > 1:2086089/1‑100 (MQ=255)
gAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGa < 2:1667058/100‑1 (MQ=255)
gAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGa > 2:2086606/1‑100 (MQ=255)
aTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACg > 2:1566550/1‑100 (MQ=255)
aTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACg > 2:1949913/1‑100 (MQ=255)
aTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACg < 1:829185/100‑1 (MQ=255)
cGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctg > 2:896912/1‑100 (MQ=255)
gCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctgc > 1:1651877/1‑100 (MQ=255)
gCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctgc > 1:1502747/1‑100 (MQ=255)
gCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctgc > 1:1967675/1‑100 (MQ=255)
gCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctgc > 2:1049480/1‑100 (MQ=255)
ccACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcg > 2:485329/1‑100 (MQ=255)
ccACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcg > 2:75468/1‑100 (MQ=255)
ccACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcg > 1:1353585/1‑100 (MQ=255)
ccACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcg < 1:278938/100‑1 (MQ=255)
cACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcgc > 1:1119828/1‑100 (MQ=255)
acgacgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCa < 2:547316/100‑1 (MQ=255)
cgacgCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCAt > 2:2403251/1‑100 (MQ=255)
acgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCt < 1:40131/100‑1 (MQ=255)
cgGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtca < 2:704075/100‑1 (MQ=255)
cgGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtca < 1:2339326/100‑1 (MQ=255)
cgGTGGGCGAACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtca < 2:2300806/100‑1 (MQ=255)
ggTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtcat > 2:1033029/1‑100 (MQ=255)
ggTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTAAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtcat > 2:2005164/1‑100 (MQ=255)
gTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtcatc < 2:2249107/100‑1 (MQ=255)
gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtcatca < 2:1967674/99‑1 (MQ=255)
gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCAc > 1:361114/1‑100 (MQ=255)
gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCAc > 1:149577/1‑100 (MQ=255)
gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCAc > 2:2099531/1‑100 (MQ=255)
ggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACt > 2:715581/1‑100 (MQ=255)
gCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCCTCTTCATCACTa > 1:696436/1‑100 (MQ=255)
gCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTa > 1:1759752/1‑100 (MQ=255)
ggACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACg > 1:1439112/1‑100 (MQ=255)
acgaaTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGcc < 1:1565676/100‑1 (MQ=255)
|
TGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCC > NC_000913/4072075‑4072266
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
