Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F55 I0 R1 245 145.6 7293262 93.0% 6782733 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,072,171 C→A 100% V161F (GTC→TTC)  yihT ← 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,072,1710CA98.3% 403.8 / ‑1.3 120V161F (GTC→TTC) yihT6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (52/66);  total (53/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 122 total aligned reads displayed.

TTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCA  >  NC_000913/4072074‑4072267
                                                                                                 |                                                                                                
ttGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATg                                                                                                <  2:3245/100‑1 (MQ=255)
 tGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg                                                                                               <  2:1102676/100‑1 (MQ=255)
 tGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg                                                                                               >  1:601426/1‑100 (MQ=255)
  gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTTTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGc                                                                                              <  2:2026560/100‑1 (MQ=255)
  gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACTACGCGGTGGGCGGACGAATGGc                                                                                              <  2:2111081/100‑1 (MQ=255)
  gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGc                                                                                              <  2:2314640/100‑1 (MQ=255)
   tAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCt                                                                                             <  1:1337666/100‑1 (MQ=255)
   tAGAGGTCAGCGCCACTGTCGCCCAGCTCTTAGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCt                                                                                             >  1:2254982/1‑100 (MQ=255)
    agagGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTc                                                                                            >  2:1870651/1‑100 (MQ=255)
    agagGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTc                                                                                            >  2:1532953/1‑100 (MQ=255)
      agGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCaa                                                                                          <  1:1706766/100‑1 (MQ=255)
       ggTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAat                                                                                         <  2:2270489/100‑1 (MQ=255)
       ggTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAat                                                                                         >  2:742194/1‑100 (MQ=255)
        gTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCCTCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatg                                                                                        <  1:1812991/100‑1 (MQ=255)
        gTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatg                                                                                        <  2:601424/100‑1 (MQ=255)
          cAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAatgat                                                                                      >  1:1275053/1‑100 (MQ=255)
           aGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatgatg                                                                                     >  2:131828/1‑100 (MQ=255)
            gcgcCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGc                                                                                    <  2:1730541/100‑1 (MQ=255)
             cgcCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCt                                                                                   <  1:3596912/100‑1 (MQ=255)
              gcCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCtt                                                                                  >  1:1348751/1‑100 (MQ=255)
                 aCTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTAcc                                                                               <  1:881551/100‑1 (MQ=255)
                   tGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAg                                                                             >  2:2746533/1‑100 (MQ=255)
                    gTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGa                                                                            <  1:3058739/100‑1 (MQ=255)
                        cccAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCgt                                                                        >  1:69161/1‑100 (MQ=255)
                         ccAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCgtg                                                                       <  2:3371632/100‑1 (MQ=255)
                           aGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGtgtg                                                                     <  1:771517/100‑1 (MQ=255)
                           aGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGtgtg                                                                     <  1:1995657/100‑1 (MQ=255)
                           aGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGtgtg                                                                     <  2:1027929/100‑1 (MQ=255)
                            gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGa                                                                    <  2:3603736/100‑1 (MQ=255)
                            gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGa                                                                    >  1:292149/1‑100 (MQ=255)
                               ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTg                                                                 <  2:521813/100‑1 (MQ=255)
                               ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTg                                                                 >  2:1625380/1‑100 (MQ=255)
                               ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTg                                                                 >  1:1950500/1‑100 (MQ=255)
                                tttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTgg                                                                >  1:1409165/1‑100 (MQ=255)
                                 ttGGCGGTATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGc                                                               <  1:2894958/100‑1 (MQ=255)
                                 ttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTggn                                                               <  2:1011675/100‑2 (MQ=255)
                                 ttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGc                                                               >  2:814528/1‑100 (MQ=255)
                                 ttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGc                                                               <  1:2024303/100‑1 (MQ=255)
                                 ttGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGc                                                               <  2:3409043/100‑1 (MQ=255)
                                    gcggcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGcaca                                                            >  1:1411565/1‑100 (MQ=255)
                                    gcggcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGcaca                                                            >  1:2276654/1‑100 (MQ=255)
                                    gcggcATCGATGATCGCTTGTTCGCGATCGAATTTATAGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGcaca                                                            >  1:3245481/1‑100 (MQ=255)
                                       gcATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGtt                                                         <  1:1625388/100‑1 (MQ=255)
                                         aTCGATGATCGCTTGTTCGCGATCGAATTTATCGCAACGACGCGGTGGGCGGGCGAATAGCTCAATGATGCTTAAGAGACCGTGTCAGTGGcacctgt                                                         >  2:2942336/1‑94 (MQ=255)
                                          tCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGt                                                      <  1:733367/100‑1 (MQ=255)
                                          tCGATGAACGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCGATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGt                                                      <  2:1950490/100‑1 (MQ=255)
                                           cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt                                                     <  1:3482851/100‑1 (MQ=255)
                                           cGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGtt                                                     <  1:1515833/100‑1 (MQ=255)
                                            gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGttn                                                    <  2:1176037/100‑2 (MQ=255)
                                            gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTg                                                    <  2:2276639/100‑1 (MQ=255)
                                            gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTg                                                    <  1:3299474/100‑1 (MQ=255)
                                            gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGGGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTg                                                    <  2:712679/100‑1 (MQ=255)
                                             atgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGa                                                   <  2:2699647/100‑1 (MQ=255)
                                              tgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGaa                                                  >  1:1250934/1‑100 (MQ=255)
                                               gatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAc                                                 <  2:32350/100‑1 (MQ=255)
                                               gatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAc                                                 <  1:2871552/100‑1 (MQ=255)
                                               gatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAc                                                 <  1:3098346/100‑1 (MQ=255)
                                                atCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAct                                                <  2:3577323/100‑1 (MQ=255)
                                                atCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAct                                                >  1:2031044/1‑100 (MQ=255)
                                                  cGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAActct                                              >  2:2695032/1‑100 (MQ=255)
                                                   gCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCtt                                             <  2:2163236/100‑1 (MQ=255)
                                                    cTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGGTGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCttt                                            >  2:2322251/1‑100 (MQ=255)
                                                    cTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCttt                                            <  1:3014268/100‑1 (MQ=255)
                                                     ttGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTc                                           <  2:2847657/100‑1 (MQ=255)
                                                      tGTTCGCTATCGATTTTATCGCCACGTCGCGGTGGTCGGACGAATGGCTTAATGATGCTTACCAGTCCGCGTGAGTGGCACAGTTCGGTGAACGCTTTCa                                          <  2:739945/100‑1 (MQ=255)
                                                      tGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTc                                           >  1:1289484/1‑99 (MQ=255)
                                                      tGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCa                                          <  1:1347177/100‑1 (MQ=255)
                                                       gTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCAc                                         <  2:1275045/100‑1 (MQ=255)
                                                       gTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCAc                                         >  2:588406/1‑100 (MQ=255)
                                                       gTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCAc                                         <  1:2009061/100‑1 (MQ=255)
                                                         tCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCa                                       <  1:1550560/100‑1 (MQ=255)
                                                          cgcgATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCat                                      >  2:2958491/1‑100 (MQ=255)
                                                           ggaagggAATTGAGCGCCGCGAGGCGGTGTGCGGAGGAATGGGTGAATTATTCTGACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCat                                      <  2:3578232/93‑1 (MQ=255)
                                                           gcgATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCata                                     >  2:3549345/1‑100 (MQ=255)
                                                            cgATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCat                                      >  2:2358114/1‑98 (MQ=255)
                                                             gATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATc                                   >  1:2049876/1‑100 (MQ=255)
                                                              aTCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATcc                                  >  2:1456814/1‑100 (MQ=255)
                                                              aTCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATcc                                  <  2:3278077/100‑1 (MQ=255)
                                                                cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAg                                <  2:719996/100‑1 (MQ=255)
                                                                  aaTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGAc                              <  1:2408660/100‑1 (MQ=255)
                                                                   aTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACg                             >  2:1444493/1‑100 (MQ=255)
                                                                      tATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtg                          >  1:60522/1‑100 (MQ=255)
                                                                       aTCGCCACGACGCGGTGGGCGGACGAATGGCTTAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgc                         <  2:2558680/100‑1 (MQ=255)
                                                                        tCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACAATATCCAGACGTtgc                         >  1:1231379/1‑99 (MQ=255)
                                                                         cGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctg                       >  1:2316112/1‑100 (MQ=255)
                                                                          gCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgctgc                      >  1:792845/1‑100 (MQ=255)
                                                                             acgacgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCa                   >  1:829457/1‑100 (MQ=255)
                                                                              cgacgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCAt                  <  1:2695050/100‑1 (MQ=255)
                                                                               nacgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATc                 >  2:1306263/2‑100 (MQ=255)
                                                                                acgCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCt                <  1:1283842/100‑1 (MQ=255)
                                                                                   cgGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtca             <  1:1842329/100‑1 (MQ=255)
                                                                                   cgGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtca             <  1:3131550/100‑1 (MQ=255)
                                                                                      tGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTTCGCATCTtcatca          <  1:2474525/100‑1 (MQ=255)
                                                                                       gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCAc         >  1:666381/1‑100 (MQ=255)
                                                                                        ggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACt        >  2:3286111/1‑100 (MQ=255)
                                                                                         gCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTa       <  1:311515/100‑1 (MQ=255)
                                                                                          cGGACTAATGGCTCAATAATGCTTACCAGACCGTGTGAGTCGCACAGTTCTTAGAACTCTTTCACCATATCCAGACGCTGCTGCGCATCtt               <  2:2248815/91‑1 (MQ=255)
                                                                                           ggACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCAGCACTACg     <  2:835593/100‑1 (MQ=255)
                                                                                             acgaaTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGcc   <  1:2486537/100‑1 (MQ=255)
                                                                                              cgaaTGGCTCAATGATGCTTACAAAACCGTGTGAGTGGCACAATTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCca  >  2:3269712/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCA  >  NC_000913/4072074‑4072267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A