Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,856 G→A 23.3% intergenic (+67/‑277) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8560GA23.3% 312.4 / 88.6 163intergenic (+67/‑277)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (59/65);  new base A (23/15);  total (82/81)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 170 total aligned reads displayed.

CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG  >  NC_000913/454757‑454955
                                                                                                   |                                                                                                   
   cccTGTCGTGGAGCAGGAAGCATCGTGTATAAGCGCATTTGCAACTGCGGGCTCTTTTCCAGAAGGTTGGTAAAGATTTTATGAAAAACGGCCTGTgg                                                                                                    >  1:366835/1‑98 (MQ=255)
   cccTGTCGTGGAGCAGGAAGCATCGCGTCAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTGGCTAAAGATTTTATGAAAAACGGCCTGCgg                                                                                                    >  1:4012134/1‑98 (MQ=255)
   cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGc                                                                                                  <  1:2545663/100‑1 (MQ=255)
     cggTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAAAAAAAAAAAAACGGCCTGCGGGcc                                                                                                 <  1:3127174/97‑1 (MQ=255)
         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                            >  2:1151322/1‑100 (MQ=255)
          gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgttttt                                                                                           <  1:1965699/100‑2 (MQ=255)
           tGGAGCAGGAGGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAACGGCCCGCAGGCCgtttttc                                                                                          <  1:2888745/100‑3 (MQ=255)
           tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttc                                                                                          <  1:3216381/100‑3 (MQ=255)
           tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttc                                                                                          <  1:3841004/100‑3 (MQ=255)
            ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                         <  2:432199/100‑4 (MQ=255)
            ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                         <  1:3245507/100‑4 (MQ=255)
            ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                         <  1:2492279/100‑4 (MQ=255)
            ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                         <  2:3378679/100‑4 (MQ=255)
             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgttt                                                                                        <  2:2798765/100‑1 (MQ=255)
             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgttt                                                                                        >  1:830913/1‑100 (MQ=255)
             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgttt                                                                                        <  2:1301537/100‑1 (MQ=255)
              aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttcat                                                                                        <  1:2056789/99‑5 (MQ=39)
               gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                      >  1:4269669/1‑100 (MQ=255)
               gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                      >  1:4058070/1‑100 (MQ=255)
               gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttcataa                                                                                      <  2:1087720/100‑7 (MQ=38)
                 aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                    <  2:3687534/100‑1 (MQ=255)
                 aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                    >  2:1816923/1‑100 (MQ=255)
                 aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttcataaaa                                                                                    <  2:2945220/100‑9 (MQ=38)
                   gaggCATCGCGTAAAAGGGCATTTGCAACTGTCGTCGCTTTTCCAGTATGTTGGTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                  <  1:1929448/97‑1 (MQ=255)
                   gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                  <  2:2151162/100‑1 (MQ=255)
                   gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                  >  2:3574389/1‑100 (MQ=255)
                   gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                  <  2:3804978/100‑1 (MQ=255)
                       cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtt                                                                              >  2:3836218/1‑100 (MQ=255)
                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAttt                                                                             <  2:3767110/100‑1 (MQ=255)
                          cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                           <  1:2301200/100‑1 (MQ=255)
                          cgcgTAAAAACGCATTTGCAACTGACGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGGGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                           >  1:2323117/1‑100 (MQ=255)
                           gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                          <  1:1027321/100‑1 (MQ=255)
                           gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                          <  2:242156/100‑1 (MQ=255)
                              tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCt                                                                       <  1:1816916/100‑1 (MQ=255)
                                 aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                    >  1:2440189/1‑100 (MQ=255)
                                    cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttttt                                                                  >  1:2872696/1‑98 (MQ=255)
                                    cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGc                                                                 >  2:1326056/1‑100 (MQ=255)
                                     gCATTTGCAACTGTCGGCGCTTTTCAAGTATGTTGCTAAAGATTTTATGAAAATCGGCCTGCTGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                  <  1:792242/98‑1 (MQ=255)
                                      cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGccc                                                               <  2:1579085/100‑1 (MQ=255)
                                      cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGccc                                                               <  2:223522/100‑1 (MQ=255)
                                      cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGccc                                                               <  2:3265852/100‑1 (MQ=255)
                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCa                                                              <  1:3761895/100‑1 (MQ=255)
                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCa                                                              <  1:1079812/100‑1 (MQ=255)
                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCCTGATCTTGCGCTTTTTGccc                                                               >  1:978387/1‑99 (MQ=255)
                                           gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                          >  2:1799783/1‑100 (MQ=255)
                                           gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                          <  2:1740563/100‑1 (MQ=255)
                                            cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                         >  2:1267770/1‑100 (MQ=255)
                                             aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                        >  1:1448841/1‑100 (MQ=255)
                                              aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                       <  1:1059468/100‑1 (MQ=255)
                                              aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAGTTTGCGCTGTTTGCCCAGCATTCa                                                       >  2:3513422/1‑100 (MQ=255)
                                               cTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                      <  2:3659881/100‑1 (MQ=255)
                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                     <  2:866331/100‑1 (MQ=255)
                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                     <  1:175650/100‑1 (MQ=255)
                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                     <  2:1635810/100‑1 (MQ=255)
                                                  tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                    <  1:1701235/99‑1 (MQ=255)
                                                    ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                 <  2:1474152/100‑1 (MQ=255)
                                                     gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                <  2:1492811/100‑1 (MQ=255)
                                                         ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTg                                            >  1:1283693/1‑100 (MQ=255)
                                                         ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTg                                            >  2:4037823/1‑100 (MQ=255)
                                                          tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGc                                           >  2:2506441/1‑100 (MQ=255)
                                                           ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGcc                                          >  1:1916952/1‑100 (MQ=255)
                                                            tCCAGTATGTTGCTAAAGATTTTATGAAAATCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGccc                                         <  1:3182128/100‑1 (MQ=255)
                                                            tCCAGTATGTTGCT‑AAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTCTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCg                                        >  1:3137322/1‑100 (MQ=255)
                                                              cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                       <  1:2109761/100‑1 (MQ=255)
                                                               aGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCggg                                      <  1:2793184/100‑1 (MQ=255)
                                                                   tGTTGCTAAAGATTTTATGAAAAACGGCCTGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAtt                                  >  2:4183267/1‑100 (MQ=255)
                                                                       gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGa                              <  2:558142/100‑1 (MQ=255)
                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaa                             >  2:2588812/1‑100 (MQ=255)
                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaa                             <  1:1590777/100‑1 (MQ=255)
                                                                          aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaa                           <  2:506398/100‑1 (MQ=255)
                                                                            aGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                         >  2:1430618/1‑100 (MQ=255)
                                                                            aGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                         <  2:3118994/100‑1 (MQ=255)
                                                                              agacaaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                       >  2:3643975/10‑100 (MQ=38)
                                                                               ttttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAc                      <  2:1975492/100‑1 (MQ=255)
                                                                                tttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcg                     <  2:2440200/100‑1 (MQ=255)
                                                                                  tATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                   >  2:4359986/1‑100 (MQ=255)
                                                                                  aaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                   >  1:430219/6‑100 (MQ=39)
                                                                                   aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                  >  2:4136885/5‑100 (MQ=39)
                                                                                   aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                  >  2:4008629/5‑100 (MQ=39)
                                                                                   aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                    >  1:3465275/5‑98 (MQ=39)
                                                                                   aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGCCTGGATTCTGCGCTTTTTGCCCAGCATTCAAACAAAAATTGCCCGGAAATTGTGAAAACATAcgc                    >  1:4059874/5‑98 (MQ=37)
                                                                                   aTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                  >  2:544543/1‑100 (MQ=255)
                                                                                    tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                 >  2:2801833/1‑100 (MQ=255)
                                                                                    tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGAGAATTGTGAAAAAATACGCGAc                 >  1:2968527/1‑100 (MQ=255)
                                                                                     gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                >  1:1151917/1‑100 (MQ=255)
                                                                                     acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                >  1:632640/3‑100 (MQ=255)
                                                                                     acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                >  1:4406131/3‑100 (MQ=255)
                                                                                     acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                >  2:2363711/3‑100 (MQ=255)
                                                                                     acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGAGCTTTTTGCTCATCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                >  1:2415652/3‑100 (MQ=255)
                                                                                      caaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAg               >  2:1473264/2‑100 (MQ=255)
                                                                                      caaaaCGGCCCGCAGGCCGTTTTGTATTGTCTGGATTTTGCGCTTTTTGAGCAGCATTCAGACGCACACGGCCCGGGCATTCTGAAAAAATCAGCGAc                 >  1:1997953/2‑98 (MQ=255)
                                                                                      aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAg               >  1:1235372/1‑100 (MQ=255)
                                                                                       aaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgc              >  2:2848288/1‑100 (MQ=255)
                                                                                       aaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgc              >  1:2113443/1‑100 (MQ=255)
                                                                                             gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa        >  2:1921832/1‑100 (MQ=255)
                                                                                              ccTGCGGGTCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt       <  1:924441/100‑1 (MQ=255)
                                                                                               cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa      <  2:4265723/100‑1 (MQ=255)
                                                                                               cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa      <  1:1605864/100‑1 (MQ=255)
                                                                                                   gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCg  >  1:1353112/1‑100 (MQ=255)
                                                                                                   gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCg  <  2:2954250/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG  >  NC_000913/454757‑454955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A