Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,007,187 A→T 32.5% D309V (GAC→GTC)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,1870AT32.5% 212.5 / 149.9 166D309V (GAC→GTC) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (59/53);  new base T (24/30);  total (83/83)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 167 total aligned reads displayed.

AAGTGATGGAAGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAG  >  NC_000913/3007088‑3007285
                                                                                                    |                                                                                                  
   tGATGGAAGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTg                                                                                                 <  1:4298268/100‑1 (MQ=255)
    gATGGAAGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTgg                                                                                                <  1:1407825/100‑1 (MQ=255)
    gATGGAAGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTgg                                                                                                <  2:238921/100‑1 (MQ=255)
         aaGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTg                                                                                           <  2:1587633/100‑1 (MQ=255)
         aaGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTg                                                                                           <  2:1910762/100‑1 (MQ=255)
         aaGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTg                                                                                           <  1:2610904/100‑1 (MQ=255)
         aaGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTg                                                                                           <  1:4011277/100‑1 (MQ=255)
          agagCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGt                                                                                          >  1:4096500/1‑100 (MQ=255)
          agagCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGt                                                                                          >  1:3005847/1‑100 (MQ=255)
            agCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTAc                                                                                        <  1:2149074/100‑1 (MQ=255)
            agCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTAc                                                                                        <  2:3635892/100‑1 (MQ=255)
            agCGTAATGAATTTCTGCGTCCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTAc                                                                                        <  1:926907/100‑1 (MQ=255)
               gTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGa                                                                                     <  1:3521086/100‑1 (MQ=255)
               gTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGa                                                                                     >  1:23848/1‑100 (MQ=255)
               gTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGa                                                                                     <  1:3221467/100‑1 (MQ=255)
                 aCTGAATTGCTGGGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAag                                                                                   <  1:991335/100‑1 (MQ=255)
                 aCTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGCACGCGCAACAC‑AAAGTCTGGCATTGTACTGAag                                                                                   >  1:450317/1‑100 (MQ=255)
                 aCTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAag                                                                                   >  1:3088197/1‑100 (MQ=255)
                 aCTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAag                                                                                   >  2:608663/1‑100 (MQ=255)
                  cTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACCGAACGCGCAACAC‑AAAGACTGACATTGTACTGAaga                                                                                  >  1:68488/1‑100 (MQ=255)
                     aaTTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAgat                                                                               <  2:2213487/100‑1 (MQ=255)
                         gctgcGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGatgatg                                                                           <  2:2140940/100‑1 (MQ=255)
                          ctgcGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATgg                                                                          <  1:1301003/100‑1 (MQ=255)
                           tgcgtgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGa                                                                         <  2:3685419/100‑1 (MQ=255)
                            gcgtgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGaa                                                                        <  2:4297704/100‑1 (MQ=255)
                             cgtgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAAc                                                                       <  2:1975063/100‑1 (MQ=255)
                              gtgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACt                                                                      <  1:1286441/100‑1 (MQ=255)
                              gtgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACt                                                                      >  2:1186450/1‑100 (MQ=255)
                               tgcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTg                                                                     >  2:3370113/1‑100 (MQ=255)
                                gcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGa                                                                    <  2:3973114/100‑1 (MQ=255)
                                gcgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGGACTGAAGAGATGATGGAACTGa                                                                    <  2:3563442/100‑1 (MQ=255)
                                 cgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGAc                                                                   >  1:2385399/1‑100 (MQ=255)
                                 cgAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGa                                                                    >  1:753339/1‑99 (MQ=255)
                                  gAACGATCACGAAGGCTTAAAAGCACTGGAGAATCAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGAcc                                                                  >  1:2895105/1‑100 (MQ=255)
                                    aCGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCg                                                                <  2:735325/100‑1 (MQ=255)
                                    aCGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCg                                                                >  2:530161/1‑100 (MQ=255)
                                    aCGATCACGAAGGCTTAAAAGCACAGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCg                                                                <  1:2754367/100‑1 (MQ=255)
                                         cACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATg                                                           >  1:2290267/1‑100 (MQ=255)
                                           cGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGc                                                         <  2:1227470/100‑1 (MQ=255)
                                            gAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCg                                                        <  2:3721509/100‑1 (MQ=255)
                                             aaGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGa                                                       <  2:1797833/100‑1 (MQ=255)
                                             aaGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGa                                                       >  2:4283260/1‑100 (MQ=255)
                                               ggCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGaa                                                      >  1:4325643/1‑99 (MQ=255)
                                               ggCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAg                                                     <  1:388683/100‑1 (MQ=255)
                                                gCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGc                                                    >  2:890983/1‑100 (MQ=255)
                                                 cTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGcc                                                   >  1:2639677/1‑100 (MQ=255)
                                                 cTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGcc                                                   >  2:2675740/1‑100 (MQ=255)
                                                  ttAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGccc                                                  <  2:2834071/100‑1 (MQ=255)
                                                   tAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCt                                                 >  2:306386/1‑100 (MQ=255)
                                                   tAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCt                                                 >  1:2166844/1‑100 (MQ=255)
                                                     aaaGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGt                                               >  1:1691973/1‑100 (MQ=255)
                                                     aaaGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGt                                               <  1:1507628/100‑1 (MQ=255)
                                                     aaaGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGt                                               >  1:2837945/1‑100 (MQ=255)
                                                      aaGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTa                                              <  2:397961/100‑1 (MQ=255)
                                                      aaGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTa                                              >  2:1125169/1‑100 (MQ=255)
                                                       aGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTAc                                             <  2:697220/100‑1 (MQ=255)
                                                        gCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACa                                            <  2:2550011/100‑1 (MQ=255)
                                                        gCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACa                                            <  2:1025542/100‑1 (MQ=255)
                                                        gCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGAGAAGCCCTGTACa                                            >  2:3943277/1‑100 (MQ=255)
                                                         cACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACAt                                           >  1:2979076/1‑100 (MQ=255)
                                                         cACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACAt                                           >  2:2876984/1‑100 (MQ=255)
                                                          aCTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATg                                          >  1:929968/1‑100 (MQ=255)
                                                           cTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGc                                         >  1:3599355/1‑100 (MQ=255)
                                                           cTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGc                                         >  2:679372/1‑100 (MQ=255)
                                                           cTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGc                                         >  2:3226798/1‑100 (MQ=255)
                                                           cTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGc                                         <  2:3776765/100‑1 (MQ=255)
                                                            tGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCa                                        >  2:3410372/1‑100 (MQ=255)
                                                             ggAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCAc                                       >  2:2625291/1‑100 (MQ=255)
                                                             ggAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACCGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCAc                                       >  1:420044/1‑100 (MQ=255)
                                                              gAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACCGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCAct                                      >  1:1039222/1‑100 (MQ=255)
                                                              gAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCAct                                      <  2:3520036/100‑1 (MQ=255)
                                                                      gtgtCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCa                              >  2:1281836/1‑100 (MQ=255)
                                                                      gtgtCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGAGGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCa                              <  1:1959508/100‑1 (MQ=255)
                                                                        gtCTGGCACATAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGCTGCCGAAGCCCTGTACATGCACTGCCTGCCAGc                            <  1:2895486/100‑1 (MQ=255)
                                                                        gtCTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGc                            <  2:3648366/100‑1 (MQ=255)
                                                                          cTGGCACAGAACGCGCAACACAAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCt                           >  2:3983561/1‑100 (MQ=255)
                                                                          cTGGCACAGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTg                          <  2:1324140/100‑1 (MQ=255)
                                                                          cTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTg                          <  1:1108070/100‑1 (MQ=255)
                                                                            ggCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGat                        <  2:4395955/100‑1 (MQ=255)
                                                                            ggCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGat                        >  2:1890518/1‑100 (MQ=255)
                                                                             gCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGata                       <  2:3122584/100‑1 (MQ=255)
                                                                               acaGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATc                     <  1:576610/100‑1 (MQ=255)
                                                                               acaGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATc                     <  2:2217650/100‑1 (MQ=255)
                                                                                caGAACGCGCAACAC‑AAAGTCTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCa                    <  1:4107722/100‑1 (MQ=255)
                                                                                 aGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAg                   <  1:147682/100‑1 (MQ=255)
                                                                                  gAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgc                  >  1:3129769/1‑100 (MQ=255)
                                                                                  gAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgc                  <  2:4235895/100‑1 (MQ=255)
                                                                                   aaCGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgcg                 <  1:3703197/100‑1 (MQ=255)
                                                                                   aaCGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgc                  <  1:1605364/99‑1 (MQ=255)
                                                                                    atgcgcAACAC‑AAAGTGTGGCATTGTACTGAAGAGATGATGAAACTTACCCATTCTGACGCATCCCGGTAGCTTCACTGCCTGCCAGCTGATATCAgcgg                >  1:1111993/3‑100 (MQ=255)
                                                                                     cgcgCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgcggc               >  1:3860020/1‑100 (MQ=255)
                                                                                      gcgcAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAgcggcg              <  2:2907161/100‑1 (MQ=255)
                                                                                        gcAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTa            >  2:1499060/1‑100 (MQ=255)
                                                                                         cAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCC‑GCTGATATCAgctggcaggc          <  2:54919/100‑9 (MQ=255)
                                                                                          aaCAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATc          <  2:3268291/100‑1 (MQ=255)
                                                                                           acac‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATcc         <  1:3272216/100‑1 (MQ=255)
                                                                                            cac‑aAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCt        >  1:969673/1‑100 (MQ=255)
                                                                                              c‑aAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGt      >  1:2955822/1‑100 (MQ=255)
                                                                                                   gACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAaag  >  1:1849469/1‑100 (MQ=255)
                                                                                                   gACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAaag  <  2:1954073/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
AAGTGATGGAAGAGCGTACTGAATTGCTGCGTGCGAACGATCACGAAGGCTTAAAAGCACTGGAAAAACAGTGTCTGGCACAGAACGCGCAACAC‑AAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAG  >  NC_000913/3007088‑3007285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A