Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F201 I0 R1	306	176.9	8831342	93.4%	8248473	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,298,809	A→G	22.8%	V13A (GTG→GCG)	yraR ←	putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,809	0	A	G	22.8%	324.1 / 102.8	180	V13A (GTG→GCG)	yraR	putative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand): ref base A (60/78); new base G (18/23); total (79/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 190 total aligned reads displayed.

TTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAG  >  NC_000913/3298710‑3298907

ttAAACCCCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCcca                                                                                                    <  1:2484855/100‑1 (MQ=255)

   aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCccacca                                                                                                 >  1:2043303/1‑100 (MQ=255)

       ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGccc                                                                                             <  1:445926/100‑1 (MQ=255)

       ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGccc                                                                                             >  1:2990345/1‑100 (MQ=255)

       ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGcc                                                                                              <  2:3429495/99‑1 (MQ=255)

         ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCg                                                                                           <  1:2125328/100‑1 (MQ=255)

           gggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGtt                                                                                         >  2:1246687/1‑100 (MQ=255)

            ggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTg                                                                                        <  1:1042295/100‑1 (MQ=255)

              cATATCGCCCAACTGACGTCGCGTCGGCGCCCCAATGGCGTTAATTTTCGGTTCGTTAATCAACATCCGCAGCAGGTCACCGCCCACCAGCCCCGTTgcg                                                                                      <  1:873588/100‑1 (MQ=255)

               atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcgc                                                                                     <  2:3213046/100‑1 (MQ=255)

               atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTgcgc                                                                                     <  2:3096933/100‑1 (MQ=255)

               atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTgcgc                                                                                     <  2:3096802/100‑1 (MQ=255)

               atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTgcgc                                                                                     >  1:2391190/1‑100 (MQ=255)

                tatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGcc                                                                                    >  1:596201/1‑100 (MQ=255)

                  tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTg                                                                                  <  2:1815453/100‑1 (MQ=255)

                   cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGt                                                                                 >  2:2169998/1‑100 (MQ=255)

                      ccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAAt                                                                              <  2:1704789/100‑1 (MQ=255)

                      ccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAAt                                                                              <  1:3465631/100‑1 (MQ=255)

                       cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATc                                                                             <  1:4070023/100‑1 (MQ=255)

                       cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATc                                                                             <  1:1019272/100‑1 (MQ=255)

                       cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATc                                                                             >  2:370314/1‑100 (MQ=255)

                       cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATc                                                                             <  2:4066878/100‑1 (MQ=255)

                        aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCa                                                                            <  2:2593177/100‑1 (MQ=255)

                        aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCa                                                                            <  2:4398108/100‑1 (MQ=255)

                        aaCGGACGTCGCGTCGGCGCAGCAATGCCGTTAACTTTCGGTCCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCa                                                                            <  1:1799888/100‑1 (MQ=255)

                            gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAg                                                                           <  1:1224296/97‑1 (MQ=255)

                              cgtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCt                                                                      <  1:1652117/100‑1 (MQ=255)

                                tcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGa                                                                    >  2:750915/1‑100 (MQ=255)

                                tcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGa                                                                    >  2:2090393/1‑100 (MQ=255)

                                 cgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                   <  2:2798826/100‑1 (MQ=255)

                                 cgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                   <  1:4259995/100‑1 (MQ=255)

                                 cgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                   <  2:3942287/100‑1 (MQ=255)

                                   cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTc                                                                 >  2:1828451/1‑100 (MQ=255)

                                    gtcgGCGCAGCAATGGCGTTAACTTTCTGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCa                                                                <  2:1634369/100‑1 (MQ=255)

                                    gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCa                                                                <  2:1970617/100‑1 (MQ=255)

                                        gcgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCa                                                                <  1:4010690/96‑1 (MQ=255)

                                         cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCtt                                                            >  1:3213839/1‑99 (MQ=255)

                                          gcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCg                                                          <  2:22500/100‑1 (MQ=255)

                                           cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                         <  2:2906189/100‑1 (MQ=255)

                                             gcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                       >  2:2338258/1‑100 (MQ=255)

                                             gcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                       >  2:3861310/1‑100 (MQ=255)

                                                    cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACa                                                <  2:2624785/100‑1 (MQ=255)

                                                     gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                               <  1:1410181/100‑1 (MQ=255)

                                                      ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGa                                              <  1:1609480/100‑1 (MQ=255)

                                                      ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGa                                              <  2:3003201/100‑1 (MQ=255)

                                                        aaCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAt                                            >  2:1165860/1‑100 (MQ=255)

                                                        aaCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAt                                            >  1:2908290/1‑100 (MQ=255)

                                                          cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                          <  1:750914/100‑1 (MQ=255)

                                                          cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                          <  1:1188839/100‑1 (MQ=255)

                                                            ttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTc                                        >  1:2642472/1‑100 (MQ=255)

                                                            ttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTc                                        <  2:1773771/100‑1 (MQ=255)

                                                               ggTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGc                                     <  2:2777806/100‑1 (MQ=255)

                                                               ggTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGc                                     >  2:1035775/1‑100 (MQ=255)

                                                                gtgcGTTAATCAACATCGGCAGCAGGTGACCGCCCACGAGCCCCGTTGCGCCTGTAAGCAGTACCTGACTCATCATCGCTCCTGTACAGAATTGTCTAcc                                    >  1:4181094/4‑100 (MQ=255)

                                                                gtccGTTAATCAACATCCGCAGCACGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGcc                                    <  1:1778719/97‑1 (MQ=255)

                                                                gTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTg                                      >  1:3771012/1‑98 (MQ=255)

                                                                gTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGcc                                    <  1:2233616/100‑1 (MQ=255)

                                                                gTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGcc                                    <  1:344/100‑1 (MQ=255)

                                                                 ttCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCt                                   >  2:1561764/1‑100 (MQ=255)

                                                                    gTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                >  1:157948/1‑100 (MQ=255)

                                                                    gTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                <  1:3864888/100‑1 (MQ=255)

                                                                      tactCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                              <  1:3899059/97‑1 (MQ=255)

                                                                      tAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                              <  2:3495106/100‑1 (MQ=255)

                                                                      tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                              <  1:1285977/100‑1 (MQ=255)

                                                                       aaTCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                             >  2:2638504/1‑100 (MQ=255)

                                                                       aaTCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                             <  1:343158/100‑1 (MQ=255)

                                                                          cAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                          >  2:3533666/1‑100 (MQ=255)

                                                                            aCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                        >  1:4095411/1‑100 (MQ=255)

                                                                            aCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                        <  2:3476384/100‑1 (MQ=255)

                                                                            aCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                        <  1:2863204/100‑1 (MQ=255)

                                                                              aTCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                      >  2:620837/1‑100 (MQ=255)

                                                                              aTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                      <  1:3654669/100‑1 (MQ=255)

                                                                               tCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCt                     >  2:253755/1‑100 (MQ=255)

                                                                               tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCt                     <  2:2918758/100‑1 (MQ=255)

                                                                                ccGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTc                    <  1:3354565/100‑1 (MQ=255)

                                                                                ccGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                      <  1:1472551/98‑1 (MQ=255)

                                                                                 cGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                   >  2:3545703/1‑100 (MQ=255)

                                                                                 cGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                   <  1:1612453/100‑1 (MQ=255)

                                                                                  gcagcaGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                  >  2:2855795/1‑100 (MQ=255)

                                                                                    agcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc                <  2:1309072/100‑1 (MQ=255)

                                                                                    agcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc                <  2:1036673/100‑1 (MQ=255)

                                                                                     gcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa               >  2:1396203/1‑100 (MQ=255)

                                                                                        ggTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTCCAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa            >  1:850580/1‑100 (MQ=255)

                                                                                        ggTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc             >  2:4134265/1‑99 (MQ=255)

                                                                                        ggTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa            <  1:1531528/100‑1 (MQ=255)

                                                                                         gTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCATTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAg           >  1:2816238/1‑100 (MQ=255)

                                                                                         gTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAg           >  2:813668/1‑100 (MQ=255)

                                                                                           gACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGc         <  1:397893/100‑1 (MQ=255)

                                                                                           gACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGc         >  1:537192/1‑100 (MQ=255)

                                                                                            aCCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCt        <  2:2481008/100‑1 (MQ=255)

                                                                                             ccGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGTCtt       >  1:2099821/1‑100 (MQ=255)

                                                                                             ccGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt       >  1:1008769/1‑100 (MQ=255)

                                                                                              cgcccgccAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa      >  2:1690651/1‑100 (MQ=255)

                                                                                              cGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa      <  2:3934915/100‑1 (MQ=255)

                                                                                               gCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTaa     >  1:3518919/1‑100 (MQ=255)

                                                                                                cccACCAGCCCCGTTGCGCCTGTAATCAGTACCTGTCTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt    >  2:500553/1‑100 (MQ=255)

                                                                                                cccACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt    >  2:2222305/1‑100 (MQ=255)

                                                                                                cccACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt    <  1:2308/100‑1 (MQ=255)

                                                                                                  caccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAg  <  1:2338248/100‑1 (MQ=255)

                                                                                                  caccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAg  >  2:609966/1‑100 (MQ=255)

TTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAG  >  NC_000913/3298710‑3298907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A