Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,214,964 A→G 65.7% I229V (ATT→GTT)  metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,9640AG65.7% 217.7 / 163.2 186I229V (ATT→GTT) metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (31/33);  new base G (63/59);  total (94/92)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 186 total aligned reads displayed.

TTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTA  >  NC_000913/4214868‑4215061
                                                                                                |                                                                                                 
tttCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTg                                                                                                <  1:508482/100‑1 (MQ=255)
      gCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGACGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtg                                                                                          >  1:395898/1‑100 (MQ=255)
      gCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtg                                                                                          >  2:3511892/1‑100 (MQ=255)
       cAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgt                                                                                         <  2:1276548/100‑1 (MQ=255)
        aGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgtg                                                                                        <  2:4029905/100‑1 (MQ=255)
         gCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGa                                                                                       >  1:2748322/1‑100 (MQ=255)
          cGTTGATTCGTGATTACACCGATCTGGAAATTCTGTCTGAGACGGGAGAAGGGGATGCATATCTGTTTGCCAGTCAAGATAGGCGCGTTGCCTTtgtgtc                                                                                      >  2:4352502/1‑98 (MQ=255)
          cGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGAc                                                                                      >  1:2839653/1‑100 (MQ=255)
             tGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg                                                                                   <  2:1906243/100‑1 (MQ=255)
              gATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGc                                                                                  <  2:2611125/100‑1 (MQ=255)
               aTTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGcc                                                                                 <  2:3846767/100‑1 (MQ=255)
                ttCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCa                                                                                >  2:1254098/1‑100 (MQ=255)
                ttCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCa                                                                                >  1:4109508/1‑100 (MQ=255)
                ttCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCa                                                                                >  2:1567962/1‑100 (MQ=255)
                  cGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATc                                                                              >  1:3022564/1‑100 (MQ=255)
                   gggATTACACCGATCTGTAAATTCTTGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATcc                                                                             <  1:3892156/98‑1 (MQ=255)
                    tGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATccc                                                                            <  2:3375483/100‑1 (MQ=255)
                      aTTACACCGATCTGGAAATTCTAGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGa                                                                          >  1:2560336/1‑100 (MQ=255)
                         acacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAata                                                                       <  1:442014/100‑1 (MQ=255)
                          cacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAatat                                                                      >  2:2106850/1‑100 (MQ=255)
                          cacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAatat                                                                      >  2:450736/1‑100 (MQ=255)
                          cacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat                                                                      >  2:3566764/1‑100 (MQ=255)
                           acCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatg                                                                     <  2:282943/100‑1 (MQ=255)
                           acCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAACatg                                                                     >  1:2170098/1‑100 (MQ=255)
                            ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                    >  1:2921504/1‑100 (MQ=255)
                            ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga                                                                    >  2:838476/1‑100 (MQ=255)
                            ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga                                                                    >  2:1850349/1‑100 (MQ=255)
                             cGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatgat                                                                   >  1:3049041/1‑100 (MQ=255)
                              gATCTGGAAATTCTGGCGGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatgatg                                                                  <  2:2054620/100‑1 (MQ=255)
                               aTCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATgc                                                                 <  1:1974081/100‑1 (MQ=255)
                               aTCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATgc                                                                 >  1:2126225/1‑100 (MQ=255)
                                 cTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATgcgc                                                               >  2:555742/1‑100 (MQ=255)
                                  tGGAAATTCTGGCAGAGACGGAAGAATGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCTGAATATGTTGCGCa                                                              >  1:3673126/1‑100 (MQ=255)
                                  tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa                                                              >  1:13486/1‑100 (MQ=255)
                                  tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa                                                              <  2:3239662/100‑1 (MQ=255)
                                  tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCAGATCTGTTTGCGAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa                                                              >  1:420420/1‑100 (MQ=255)
                                   ggAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCaa                                                             >  2:688470/1‑100 (MQ=255)
                                    gAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCaaa                                                            >  1:117538/1‑100 (MQ=255)
                                      aaTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACg                                                          <  2:374649/100‑1 (MQ=255)
                                       aTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGc                                                         >  1:3200692/1‑100 (MQ=255)
                                          cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTgg                                                      >  1:1466617/1‑100 (MQ=255)
                                          cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTgg                                                      <  2:2860010/100‑1 (MQ=255)
                                          cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg                                                       >  1:2325548/1‑99 (MQ=255)
                                           tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgc                                                     <  1:3348413/100‑1 (MQ=255)
                                           tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgc                                                     <  2:2813176/100‑1 (MQ=255)
                                             gCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGtcgc                                                   >  2:667150/1‑100 (MQ=255)
                                              cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCa                                                  >  2:3172826/1‑100 (MQ=255)
                                                gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAGGATAAGCTCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAgg                                                <  1:432791/100‑1 (MQ=255)
                                                gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAgg                                                <  2:3868409/100‑1 (MQ=255)
                                                 agaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGa                                               >  1:242773/1‑100 (MQ=255)
                                                 agaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGa                                               >  1:252503/1‑100 (MQ=255)
                                                  gaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGaa                                              <  2:2270623/100‑1 (MQ=255)
                                                   aCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAt                                             >  1:1758970/1‑100 (MQ=255)
                                                    cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtt                                            <  1:271513/100‑1 (MQ=255)
                                                      gaagaaGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttt                                          >  2:155840/1‑100 (MQ=255)
                                                      gaagaaGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCCAGCGTTTGCGCatcatatt                                           <  1:1936809/99‑8 (MQ=38)
                                                       aagaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAttttt                                         <  1:562477/100‑1 (MQ=255)
                                                        agaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAttttt                                         >  1:2999707/1‑99 (MQ=255)
                                                          aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg                                      <  2:4133744/100‑1 (MQ=255)
                                                             gggATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGa                                   <  2:28293/100‑1 (MQ=255)
                                                                 tGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtg                                 >  1:3946195/1‑98 (MQ=255)
                                                                 tGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCGTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg                               <  1:4391465/100‑1 (MQ=255)
                                                                  gCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTgg                              <  2:4217333/100‑1 (MQ=255)
                                                                  gCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTgg                              <  2:1298742/100‑1 (MQ=255)
                                                                   cATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                             >  2:3666007/1‑100 (MQ=255)
                                                                   cATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtgag                             >  1:103249/1‑98 (MQ=255)
                                                                   cATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                             <  2:1510123/100‑1 (MQ=255)
                                                                     tatCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg                           >  1:1440146/1‑100 (MQ=255)
                                                                       tCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGcc                         <  2:1107228/100‑1 (MQ=255)
                                                                        cTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg                        <  2:2086556/100‑1 (MQ=255)
                                                                        cTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg                        <  2:1449616/100‑1 (MQ=255)
                                                                          gTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGCCGGGCCATCCCGAATATGATGTGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGATGCCgg                       >  2:1342581/1‑99 (MQ=255)
                                                                          gTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGa                      <  1:2263188/100‑1 (MQ=255)
                                                                           tttGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAc                     <  1:3246644/100‑1 (MQ=255)
                                                                           tttGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAc                     <  1:889803/100‑1 (MQ=255)
                                                                            ttGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt                    <  2:2573522/100‑1 (MQ=255)
                                                                            ttGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt                    <  2:2651828/100‑1 (MQ=255)
                                                                            ttGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt                    <  1:2284951/100‑1 (MQ=255)
                                                                            ttGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt                    <  2:1181618/100‑1 (MQ=255)
                                                                             tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGa                      <  1:2554468/97‑1 (MQ=255)
                                                                             tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt                    <  2:850622/99‑1 (MQ=255)
                                                                             tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTa                   >  2:3034165/1‑100 (MQ=255)
                                                                              gCCAGTAAAGATAAGCGCGTTGCCTTTGTGTCGGGCCATCCCGAATATGATGCGCAAACGCAGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg                  >  1:3589681/1‑100 (MQ=255)
                                                                              gCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg                  >  2:540309/1‑100 (MQ=255)
                                                                              gCCAGTAAAAATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATCATGCGCAAACGCTGTCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg                  >  1:753461/1‑100 (MQ=255)
                                                                               ccAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa                 >  2:742447/1‑100 (MQ=255)
                                                                               ccAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa                 <  1:2069402/100‑1 (MQ=255)
                                                                                cAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAc                <  2:3361912/100‑1 (MQ=255)
                                                                                  gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAccc              <  2:2483647/100‑1 (MQ=255)
                                                                                     aaGATAAGCGCGTTGCCTTTGTGACGTGCCATCGCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGGCGGACTAGACCCgta           <  1:860609/100‑3 (MQ=255)
                                                                                     aaGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGa           <  2:2389673/100‑1 (MQ=255)
                                                                                     aaGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGa           <  2:1359961/100‑1 (MQ=255)
                                                                                       gATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATg         <  2:3620859/100‑1 (MQ=255)
                                                                                       gATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATg         >  2:599267/1‑100 (MQ=255)
                                                                                         tAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTa       >  2:1717229/1‑100 (MQ=255)
                                                                                          aaGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACGCGGATGTAc      >  1:322561/1‑100 (MQ=255)
                                                                                          aaGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTAc      <  1:1143231/100‑1 (MQ=255)
                                                                                           aGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTAcc     >  1:162333/1‑100 (MQ=255)
                                                                                           aGCGCGTTGCCTATGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTAcc     >  2:2904225/1‑100 (MQ=255)
                                                                                              gcATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGta  <  1:3782476/100‑1 (MQ=255)
                                                                                                |                                                                                                 
TTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTA  >  NC_000913/4214868‑4215061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A